The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia

Gas vesicles (GVs) are proteinaceous, gas-filled organelles used by some bacteria to enable upward movement into favorable air/liquid interfaces in aquatic environments. Serratia sp. ATCC39006 (S39006) was the first enterobacterium discovered to produce GVs naturally. The regulation of GV assembly...

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Main Authors:	Lee, Chin Mei, Monson, Rita E., Adams, Rachel M., Salmond, George P. C.
Format:	Article
Language:	English
Published:	Frontiers Media 2017
Subjects:	QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/18721/ http://umpir.ump.edu.my/id/eprint/18721/ http://umpir.ump.edu.my/id/eprint/18721/ http://umpir.ump.edu.my/id/eprint/18721/1/fmicb-08-01678.pdf

id	ump-18721
recordtype	eprints
spelling	ump-187212018-01-23T01:56:53Z http://umpir.ump.edu.my/id/eprint/18721/ The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia Lee, Chin Mei Monson, Rita E. Adams, Rachel M. Salmond, George P. C. QR Microbiology Gas vesicles (GVs) are proteinaceous, gas-filled organelles used by some bacteria to enable upward movement into favorable air/liquid interfaces in aquatic environments. Serratia sp. ATCC39006 (S39006) was the first enterobacterium discovered to produce GVs naturally. The regulation of GV assembly in this host is complex and part of a wider regulatory network affecting various phenotypes, including antibiotic biosynthesis. To identify new regulators of GVs, a comprehensive mutant library containing 71,000 insertion mutants was generated by random transposon mutagenesis and 311 putative GV-defectivemutants identified. Three of thesemutants were found to have a transposon inserted in a LacI family transcription regulator gene (rbsR) of the putative ribose operon. Each of these rbsR mutants was GV-defective; no GVs were visible by phase contrast microscopy (PCM) or transmission electron microscopy (TEM). GV deficiency was caused by the reduction of gvpA1 and gvrA transcription (the first genes of the two contiguous operons in the GV gene locus). Our results also showed that a mutation in rbsR was highly pleiotropic; the production of two secondary metabolites (carbapenem and prodigiosin antibiotics) was abolished. Interestingly, the intrinsic resistance to the carbapenem antibiotic was not affected by the rbsR mutation. In addition, the production of a siderophore, cellulase and plant virulence was reduced in the mutant, whereas it exhibited increased swimming and swarming motility. The RbsR protein was predicted to bind to regions upstreamof at least 18 genes in S39006 including rbsD (the first gene of the ribose operon) and gvrA. Electrophoretic mobility shift assays (EMSA) confirmed that RbsR bound to DNA sequences upstream of rbsD, but not gvrA. The results of this study indicate that RbsR is a global regulator that affects the modulation of GV biogenesis, but also with complex pleiotropic physiological impacts in S39006. Frontiers Media 2017 Article PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/18721/1/fmicb-08-01678.pdf Lee, Chin Mei and Monson, Rita E. and Adams, Rachel M. and Salmond, George P. C. (2017) The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia. Frontiers in Microbiology, 8. pp. 1-14. ISSN 1664-302X https://doi.org/10.3389/fmicb.2017.01678 doi: 10.3389/fmicb.2017.01678
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology
spellingShingle	QR Microbiology Lee, Chin Mei Monson, Rita E. Adams, Rachel M. Salmond, George P. C. The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia
description	Gas vesicles (GVs) are proteinaceous, gas-filled organelles used by some bacteria to enable upward movement into favorable air/liquid interfaces in aquatic environments. Serratia sp. ATCC39006 (S39006) was the first enterobacterium discovered to produce GVs naturally. The regulation of GV assembly in this host is complex and part of a wider regulatory network affecting various phenotypes, including antibiotic biosynthesis. To identify new regulators of GVs, a comprehensive mutant library containing 71,000 insertion mutants was generated by random transposon mutagenesis and 311 putative GV-defectivemutants identified. Three of thesemutants were found to have a transposon inserted in a LacI family transcription regulator gene (rbsR) of the putative ribose operon. Each of these rbsR mutants was GV-defective; no GVs were visible by phase contrast microscopy (PCM) or transmission electron microscopy (TEM). GV deficiency was caused by the reduction of gvpA1 and gvrA transcription (the first genes of the two contiguous operons in the GV gene locus). Our results also showed that a mutation in rbsR was highly pleiotropic; the production of two secondary metabolites (carbapenem and prodigiosin antibiotics) was abolished. Interestingly, the intrinsic resistance to the carbapenem antibiotic was not affected by the rbsR mutation. In addition, the production of a siderophore, cellulase and plant virulence was reduced in the mutant, whereas it exhibited increased swimming and swarming motility. The RbsR protein was predicted to bind to regions upstreamof at least 18 genes in S39006 including rbsD (the first gene of the ribose operon) and gvrA. Electrophoretic mobility shift assays (EMSA) confirmed that RbsR bound to DNA sequences upstream of rbsD, but not gvrA. The results of this study indicate that RbsR is a global regulator that affects the modulation of GV biogenesis, but also with complex pleiotropic physiological impacts in S39006.
format	Article
author	Lee, Chin Mei Monson, Rita E. Adams, Rachel M. Salmond, George P. C.
author_facet	Lee, Chin Mei Monson, Rita E. Adams, Rachel M. Salmond, George P. C.
author_sort	Lee, Chin Mei
title	The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia
title_short	The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia
title_full	The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia
title_fullStr	The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia
title_full_unstemmed	The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia
title_sort	laci-family transcription factor, rbsr, is a pleiotropic regulator of motility, virulence, siderophore and antibiotic production, gas vesicle morphogenesis and flotation in serratia
publisher	Frontiers Media
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/18721/ http://umpir.ump.edu.my/id/eprint/18721/ http://umpir.ump.edu.my/id/eprint/18721/ http://umpir.ump.edu.my/id/eprint/18721/1/fmicb-08-01678.pdf
first_indexed	2023-09-18T22:26:40Z
last_indexed	2023-09-18T22:26:40Z
_version_	1777416012361105408

The LacI-Family Transcription Factor, RbsR, is a Pleiotropic Regulator of Motility, Virulence, Siderophore and Antibiotic Production, Gas Vesicle Morphogenesis and Flotation in Serratia

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