Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks

Growth pattern of Pseudomonas putida (ATCC 49128), was found to predominantly rely on the age of the inoculums, prior to its contact with physical and chemical agents and nutrient availability. Under suitable inoculums, bacteria tend to grow faster in a batch type of growth pattern which is usually...

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Main Authors:	Abdul Aziz, Mohd Azoddein, Mani Malam, Ahmad, R. M., Yunus, Nik Meriam, Nik Sulaiman
Format:	Conference or Workshop Item
Language:	English English
Published:	EDP Sciences 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/18864/ http://umpir.ump.edu.my/id/eprint/18864/ http://umpir.ump.edu.my/id/eprint/18864/1/Effect%20of%20acclimatization%20time%20to%20microbial%20cell.pdf http://umpir.ump.edu.my/id/eprint/18864/2/Effect%20of%20acclimatization%20time%20to%20microbial%20cell%20growth%20and%20biosynthesis%20of%20mesophilic%20gammaproteobacterium%2C%20in%20orbital%20shake%20flasks%201.pdf

id	ump-18864
recordtype	eprints
spelling	ump-188642018-07-17T06:19:04Z http://umpir.ump.edu.my/id/eprint/18864/ Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks Abdul Aziz, Mohd Azoddein Mani Malam, Ahmad R. M., Yunus Nik Meriam, Nik Sulaiman TP Chemical technology Growth pattern of Pseudomonas putida (ATCC 49128), was found to predominantly rely on the age of the inoculums, prior to its contact with physical and chemical agents and nutrient availability. Under suitable inoculums, bacteria tend to grow faster in a batch type of growth pattern which is usually sustained until after nutrient depletion. In this research, the bacterial growth pattern was studied in an incubator shake flask using 8 g nutrient media and physical operational parameters temperature of 37oC and agitation of 180 rpm over a period of 24, 48 and 72 hours. Prior to this, P. putida was added into 20.0 ml nutrient broth and incubated in an incubator for 24 hours at 37oC, before adding it to 180 ml nutrient broth 30% (v/v1-). Growth, via acclimatization was initially observed after 1hr of inoculation with an overwhelming exponential growth of 2.69-2.57 within first 24 hr, exceeding the 48 and 72 hrs ranges. This additionally relates to particular cell biomass growth rate (μ) of 0.58 hr1-, 3.87 number of generation (n), generation time (g) 1.09 and growth rate constant (k) of 0.01 hr1-, achievable within 24 hrs. It was therefore concluded that the sensitivity of this strain to time is significant, as optimal growth was achieved within 24 hrs of acclimatization, thereby showing a drastic reduction in the time of growth. EDP Sciences 2017-05 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/18864/1/Effect%20of%20acclimatization%20time%20to%20microbial%20cell.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/18864/2/Effect%20of%20acclimatization%20time%20to%20microbial%20cell%20growth%20and%20biosynthesis%20of%20mesophilic%20gammaproteobacterium%2C%20in%20orbital%20shake%20flasks%201.pdf Abdul Aziz, Mohd Azoddein and Mani Malam, Ahmad and R. M., Yunus and Nik Meriam, Nik Sulaiman (2017) Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks. In: 2nd International Conference on Materials Science and Nanotechnology, ICMSNT 2017 and 2017 2nd International Symposium on Material Science and Technology, ISMST 2017, 19-22 April 2017 , Auckland University of Technology(AUT), Auckland, New Zealand. pp. 1-6., 109 (04003). ISSN 2261-236X https://doi.org/10.1051/matecconf/201710904003
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Abdul Aziz, Mohd Azoddein Mani Malam, Ahmad R. M., Yunus Nik Meriam, Nik Sulaiman Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks
description	Growth pattern of Pseudomonas putida (ATCC 49128), was found to predominantly rely on the age of the inoculums, prior to its contact with physical and chemical agents and nutrient availability. Under suitable inoculums, bacteria tend to grow faster in a batch type of growth pattern which is usually sustained until after nutrient depletion. In this research, the bacterial growth pattern was studied in an incubator shake flask using 8 g nutrient media and physical operational parameters temperature of 37oC and agitation of 180 rpm over a period of 24, 48 and 72 hours. Prior to this, P. putida was added into 20.0 ml nutrient broth and incubated in an incubator for 24 hours at 37oC, before adding it to 180 ml nutrient broth 30% (v/v1-). Growth, via acclimatization was initially observed after 1hr of inoculation with an overwhelming exponential growth of 2.69-2.57 within first 24 hr, exceeding the 48 and 72 hrs ranges. This additionally relates to particular cell biomass growth rate (μ) of 0.58 hr1-, 3.87 number of generation (n), generation time (g) 1.09 and growth rate constant (k) of 0.01 hr1-, achievable within 24 hrs. It was therefore concluded that the sensitivity of this strain to time is significant, as optimal growth was achieved within 24 hrs of acclimatization, thereby showing a drastic reduction in the time of growth.
format	Conference or Workshop Item
author	Abdul Aziz, Mohd Azoddein Mani Malam, Ahmad R. M., Yunus Nik Meriam, Nik Sulaiman
author_facet	Abdul Aziz, Mohd Azoddein Mani Malam, Ahmad R. M., Yunus Nik Meriam, Nik Sulaiman
author_sort	Abdul Aziz, Mohd Azoddein
title	Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks
title_short	Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks
title_full	Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks
title_fullStr	Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks
title_full_unstemmed	Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks
title_sort	effect of acclimatization time to microbial cell growth and biosynthesis of mesophilic gammaproteobacterium, in orbital shake flasks
publisher	EDP Sciences
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/18864/ http://umpir.ump.edu.my/id/eprint/18864/ http://umpir.ump.edu.my/id/eprint/18864/1/Effect%20of%20acclimatization%20time%20to%20microbial%20cell.pdf http://umpir.ump.edu.my/id/eprint/18864/2/Effect%20of%20acclimatization%20time%20to%20microbial%20cell%20growth%20and%20biosynthesis%20of%20mesophilic%20gammaproteobacterium%2C%20in%20orbital%20shake%20flasks%201.pdf
first_indexed	2023-09-18T22:26:55Z
last_indexed	2023-09-18T22:26:55Z
_version_	1777416028482961408

Effect of Acclimatization Time to Microbial Cell Growth and Biosynthesis of Mesophilic Gammaproteobacterium, in Orbital Shake Flasks

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