Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation

Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation

This study aims to produce succinic acid from glycerol residue through anaerobic fermentation process. The optimum condition of pH, mass substrate, and temperature was determined using response surface methodology (RSM). The concentration of glycerol and succinic acid were determined us...

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Main Authors: Nik Nor Aziati, Abdul Aziz, Mimi Sakinah, A. M.
Format: Article
Language:English
Published: Universiti Malaysia Pahang 2017
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/19138/
http://umpir.ump.edu.my/id/eprint/19138/
http://umpir.ump.edu.my/id/eprint/19138/
http://umpir.ump.edu.my/id/eprint/19138/1/fkksa-2017-mimi-Optimization%20Of%20Glycerol%20Recovery%20From%20Pretreatment.pdf
id ump-19138
recordtype eprints
spelling ump-191382017-11-21T03:41:46Z http://umpir.ump.edu.my/id/eprint/19138/ Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation Nik Nor Aziati, Abdul Aziz Mimi Sakinah, A. M. TP Chemical technology This study aims to produce succinic acid from glycerol residue through anaerobic fermentation process. The optimum condition of pH, mass substrate, and temperature was determined using response surface methodology (RSM). The concentration of glycerol and succinic acid were determined using HPLC analysis. The FTIR spectrometry was applied to examine the adsorption of organic element. Optimization of fermentation condition using RSM yielded glycerol of 159.312 g/L at pH 2, 35 °C, and 110.36 g of substrate. Succinic acid was the product of fermentation by Escherichia coli type k-12 under 19.67 g/L initial glycerol concentration, 200 rpm rotational speed, and 37 °C. The fermentation with treated glycerol generated 0.66983 g/L of succinic acid, compared to commercial glycerol which generated 0.73337 g/L of succinic acid. Overall, this research show that glycerol waste can be used as the carbon source to produce succinic acid by implementing fermentation of Escherichia coli type k-12. From the fermentation process, succinic acid was not the major product. Further study in genetic modification of Escherichia coli is highly suggested to produce succinic acid as the major product, as well as to improve the fermentation process. Universiti Malaysia Pahang 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19138/1/fkksa-2017-mimi-Optimization%20Of%20Glycerol%20Recovery%20From%20Pretreatment.pdf Nik Nor Aziati, Abdul Aziz and Mimi Sakinah, A. M. (2017) Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation. Journal of Chemical Engineering and Industrial Biotechnology (JCEIB), 2. pp. 27-36. ISSN 0126-8139 http://jceib.ump.edu.my/index.php/en/download/volume2-2017/41-optimization-of-glycerol-recovery-from-pretreatment-process-to-produce-succinic-acid-via-anaerobic-fermentation-page-27-36/file DOI: https://doi.org/10.15282/JCEIB-V2-01.29/9/2017/2.2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation
description This study aims to produce succinic acid from glycerol residue through anaerobic fermentation process. The optimum condition of pH, mass substrate, and temperature was determined using response surface methodology (RSM). The concentration of glycerol and succinic acid were determined using HPLC analysis. The FTIR spectrometry was applied to examine the adsorption of organic element. Optimization of fermentation condition using RSM yielded glycerol of 159.312 g/L at pH 2, 35 °C, and 110.36 g of substrate. Succinic acid was the product of fermentation by Escherichia coli type k-12 under 19.67 g/L initial glycerol concentration, 200 rpm rotational speed, and 37 °C. The fermentation with treated glycerol generated 0.66983 g/L of succinic acid, compared to commercial glycerol which generated 0.73337 g/L of succinic acid. Overall, this research show that glycerol waste can be used as the carbon source to produce succinic acid by implementing fermentation of Escherichia coli type k-12. From the fermentation process, succinic acid was not the major product. Further study in genetic modification of Escherichia coli is highly suggested to produce succinic acid as the major product, as well as to improve the fermentation process.
format Article
author Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
author_facet Nik Nor Aziati, Abdul Aziz
Mimi Sakinah, A. M.
author_sort Nik Nor Aziati, Abdul Aziz
title Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation
title_short Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation
title_full Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation
title_fullStr Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation
title_full_unstemmed Optimization Of Glycerol Recovery From Pretreatment Process To Produce Succinic Acid Via Anaerobic Fermentation
title_sort optimization of glycerol recovery from pretreatment process to produce succinic acid via anaerobic fermentation
publisher Universiti Malaysia Pahang
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/19138/
http://umpir.ump.edu.my/id/eprint/19138/
http://umpir.ump.edu.my/id/eprint/19138/
http://umpir.ump.edu.my/id/eprint/19138/1/fkksa-2017-mimi-Optimization%20Of%20Glycerol%20Recovery%20From%20Pretreatment.pdf
first_indexed 2023-09-18T22:27:25Z
last_indexed 2023-09-18T22:27:25Z
_version_ 1777416059725283328

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