Lipase Production and Optimization from Bioremediation of Disposed Engine Oil

Engine oil is one of the several refined products of crude oil made up of long- chain saturated hydrocarbon (base oil) additives. There are several ways engine oil can enter the environment, such as leaking oil tanks, cleaning of tanks by merchants, warships carrying engine oil, as well as the activ...

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Main Authors:	Mahmood, Mahmood H., Yang, Zhi, Thanoon, Raid D., Makky, Essam A., Mohd Hasbi, Ab. Rahim
Format:	Article
Language:	English
Published:	JOCPR 2017
Subjects:	Q Science (General) QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/19674/ http://umpir.ump.edu.my/id/eprint/19674/ http://umpir.ump.edu.my/id/eprint/19674/1/JOCPR_2017.pdf

id	ump-19674
recordtype	eprints
spelling	ump-196742020-02-28T01:26:40Z http://umpir.ump.edu.my/id/eprint/19674/ Lipase Production and Optimization from Bioremediation of Disposed Engine Oil Mahmood, Mahmood H. Yang, Zhi Thanoon, Raid D. Makky, Essam A. Mohd Hasbi, Ab. Rahim Q Science (General) QR Microbiology Engine oil is one of the several refined products of crude oil made up of long- chain saturated hydrocarbon (base oil) additives. There are several ways engine oil can enter the environment, such as leaking oil tanks, cleaning of tanks by merchants, warships carrying engine oil, as well as the activities of auto mechanics. Engine oil is a common industrial waste that has harmful environmental and health effects. This study aims to produce lipase enzyme from disposed engine oil (DEO) using a microbial degradation approach. The hydrocarbon-degrading bacterium, GS-3 was isolated from an oil-contaminated area in Kuantan, the capital city of Pahang, a state in Malaysia. Upon GC-MS analysis, it was revealed that GS-3 was able to produce methyl-3, 4, 5-trimethoxy-2, 6-dinitrobenzoate, an organic acid from the DEO. Besides, GS-3 recorded the highest lipase activity of 0.097 ± 0.007 U/ml/min within the first 24 h of activity, using DEO as the only carbon source. The FTIR data revealed the presence of a new broadband at 3421 cm-1 wavelength attributed to the stretching of the O-H group, though a new band occurred at 3424 cm-1 and 1645 cm-1 wavelength after the bioremediation process. The subsequent optimization of the process parameters for the production of lipase revealed that the bacteria perform optimally when the DEO was used as the sole carbon source at the concentration of 4% (v/v), with pH and temperature values of 7.0 and 30°C, respectively. Urea was the best source of nitrogen within the first 24 h of incubation; the addition of Tween 80, a surfactant, enhanced the production of lipase. In conclusion, lipase enzyme production through a microbial process could be a better way to manage DEO and other oil-related environmental contaminants. JOCPR 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19674/1/JOCPR_2017.pdf Mahmood, Mahmood H. and Yang, Zhi and Thanoon, Raid D. and Makky, Essam A. and Mohd Hasbi, Ab. Rahim (2017) Lipase Production and Optimization from Bioremediation of Disposed Engine Oil. Journal of Chemical and Pharmaceutical Research (JOCPR), 9 (6). pp. 26-36. ISSN 0975-7384 http://www.jocpr.com/abstract/lipase-production-and-optimization-from-bioremediation-of-disposed-engine-oil-8883.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) QR Microbiology
spellingShingle	Q Science (General) QR Microbiology Mahmood, Mahmood H. Yang, Zhi Thanoon, Raid D. Makky, Essam A. Mohd Hasbi, Ab. Rahim Lipase Production and Optimization from Bioremediation of Disposed Engine Oil
description	Engine oil is one of the several refined products of crude oil made up of long- chain saturated hydrocarbon (base oil) additives. There are several ways engine oil can enter the environment, such as leaking oil tanks, cleaning of tanks by merchants, warships carrying engine oil, as well as the activities of auto mechanics. Engine oil is a common industrial waste that has harmful environmental and health effects. This study aims to produce lipase enzyme from disposed engine oil (DEO) using a microbial degradation approach. The hydrocarbon-degrading bacterium, GS-3 was isolated from an oil-contaminated area in Kuantan, the capital city of Pahang, a state in Malaysia. Upon GC-MS analysis, it was revealed that GS-3 was able to produce methyl-3, 4, 5-trimethoxy-2, 6-dinitrobenzoate, an organic acid from the DEO. Besides, GS-3 recorded the highest lipase activity of 0.097 ± 0.007 U/ml/min within the first 24 h of activity, using DEO as the only carbon source. The FTIR data revealed the presence of a new broadband at 3421 cm-1 wavelength attributed to the stretching of the O-H group, though a new band occurred at 3424 cm-1 and 1645 cm-1 wavelength after the bioremediation process. The subsequent optimization of the process parameters for the production of lipase revealed that the bacteria perform optimally when the DEO was used as the sole carbon source at the concentration of 4% (v/v), with pH and temperature values of 7.0 and 30°C, respectively. Urea was the best source of nitrogen within the first 24 h of incubation; the addition of Tween 80, a surfactant, enhanced the production of lipase. In conclusion, lipase enzyme production through a microbial process could be a better way to manage DEO and other oil-related environmental contaminants.
format	Article
author	Mahmood, Mahmood H. Yang, Zhi Thanoon, Raid D. Makky, Essam A. Mohd Hasbi, Ab. Rahim
author_facet	Mahmood, Mahmood H. Yang, Zhi Thanoon, Raid D. Makky, Essam A. Mohd Hasbi, Ab. Rahim
author_sort	Mahmood, Mahmood H.
title	Lipase Production and Optimization from Bioremediation of Disposed Engine Oil
title_short	Lipase Production and Optimization from Bioremediation of Disposed Engine Oil
title_full	Lipase Production and Optimization from Bioremediation of Disposed Engine Oil
title_fullStr	Lipase Production and Optimization from Bioremediation of Disposed Engine Oil
title_full_unstemmed	Lipase Production and Optimization from Bioremediation of Disposed Engine Oil
title_sort	lipase production and optimization from bioremediation of disposed engine oil
publisher	JOCPR
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/19674/ http://umpir.ump.edu.my/id/eprint/19674/ http://umpir.ump.edu.my/id/eprint/19674/1/JOCPR_2017.pdf
first_indexed	2023-09-18T22:28:10Z
last_indexed	2023-09-18T22:28:10Z
_version_	1777416106374332416

Lipase Production and Optimization from Bioremediation of Disposed Engine Oil

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