A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion

A microspheres-based microbial optosensor for NO2− ion quantitation was constructed by using immobilized Raoutella planticola (R. planticola), the bacterium expressing NAD(P)H nitrite reductase (NiR) enzyme, which was isolated from local edible bird’s nest (EBN) via microbial technique. The whole ce...

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Main Authors:	Siti Nur Syazni, Mohd Zuki, Ling, Tanling, Nina Suhaity, Azmi, Lee, Yook Heng, K. F., Chong, Saiful Nizam, Tajuddin
Format:	Article
Language:	English
Published:	Elsevier Ltd 2018
Subjects:	Q Science (General) QD Chemistry QR Microbiology
Online Access:	http://umpir.ump.edu.my/id/eprint/19852/ http://umpir.ump.edu.my/id/eprint/19852/ http://umpir.ump.edu.my/id/eprint/19852/ http://umpir.ump.edu.my/id/eprint/19852/1/fist-2018-nina-whole%20cell%20bio-optode%20based%20on%20immobilized%20nitrite-degrading.pdf

id	ump-19852
recordtype	eprints
spelling	ump-198522018-07-25T03:11:03Z http://umpir.ump.edu.my/id/eprint/19852/ A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion Siti Nur Syazni, Mohd Zuki Ling, Tanling Nina Suhaity, Azmi Lee, Yook Heng K. F., Chong Saiful Nizam, Tajuddin Q Science (General) QD Chemistry QR Microbiology A microspheres-based microbial optosensor for NO2− ion quantitation was constructed by using immobilized Raoutella planticola (R. planticola), the bacterium expressing NAD(P)H nitrite reductase (NiR) enzyme, which was isolated from local edible bird’s nest (EBN) via microbial technique. The whole cells and the lipophilic Nile Blue chromoionophore (NBC) were physically adsorbed on the self-adhesive photocurable poly(n-butyl acrylate-co-N-acryloxysuccinimide) [poly(nBA-NAS)] microspheres, whilst the reduced co-enzyme NAD(P)H was covalently immobilized on the succinimide-functionalized acrylic microspheres via peptide link to produce a reagentless nitrite biosensing system. As the microbial bio-optode responded to nitrite through colour change from blue to pink, a facile reflectometric approach was adopted to measure reflectance intensity at 639 nm, before and after reaction with nitrite at optimum pH 8. The optosensor could quantify NO2− ion concentration within a dynamic linear response range of 0.5–400 mg L−1 with a limit of detection (LOD) of 0.2 mg L−1. The large surface area to volume ratio of the acrylic microspheres allowed solid-state diffusional mass transfer of the substrate to occur at micro-bio-optode surface, and an equilibrium response was achieved within 5 min. The practical feasibility of using the bio-optode for nitrite assay in food matrix sample showed good agreement with standard ion chromatography method. Elsevier Ltd 2018-09-18 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19852/1/fist-2018-nina-whole%20cell%20bio-optode%20based%20on%20immobilized%20nitrite-degrading.pdf Siti Nur Syazni, Mohd Zuki and Ling, Tanling and Nina Suhaity, Azmi and Lee, Yook Heng and K. F., Chong and Saiful Nizam, Tajuddin (2018) A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion. Sensors and Actuators B: Chemical, 255 (Part 3). pp. 2844-2852. ISSN 0925-4005 https://doi.org/10.1016/j.snb.2017.09.102 10.1016/j.snb.2017.09.102
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) QD Chemistry QR Microbiology
spellingShingle	Q Science (General) QD Chemistry QR Microbiology Siti Nur Syazni, Mohd Zuki Ling, Tanling Nina Suhaity, Azmi Lee, Yook Heng K. F., Chong Saiful Nizam, Tajuddin A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion
description	A microspheres-based microbial optosensor for NO2− ion quantitation was constructed by using immobilized Raoutella planticola (R. planticola), the bacterium expressing NAD(P)H nitrite reductase (NiR) enzyme, which was isolated from local edible bird’s nest (EBN) via microbial technique. The whole cells and the lipophilic Nile Blue chromoionophore (NBC) were physically adsorbed on the self-adhesive photocurable poly(n-butyl acrylate-co-N-acryloxysuccinimide) [poly(nBA-NAS)] microspheres, whilst the reduced co-enzyme NAD(P)H was covalently immobilized on the succinimide-functionalized acrylic microspheres via peptide link to produce a reagentless nitrite biosensing system. As the microbial bio-optode responded to nitrite through colour change from blue to pink, a facile reflectometric approach was adopted to measure reflectance intensity at 639 nm, before and after reaction with nitrite at optimum pH 8. The optosensor could quantify NO2− ion concentration within a dynamic linear response range of 0.5–400 mg L−1 with a limit of detection (LOD) of 0.2 mg L−1. The large surface area to volume ratio of the acrylic microspheres allowed solid-state diffusional mass transfer of the substrate to occur at micro-bio-optode surface, and an equilibrium response was achieved within 5 min. The practical feasibility of using the bio-optode for nitrite assay in food matrix sample showed good agreement with standard ion chromatography method.
format	Article
author	Siti Nur Syazni, Mohd Zuki Ling, Tanling Nina Suhaity, Azmi Lee, Yook Heng K. F., Chong Saiful Nizam, Tajuddin
author_facet	Siti Nur Syazni, Mohd Zuki Ling, Tanling Nina Suhaity, Azmi Lee, Yook Heng K. F., Chong Saiful Nizam, Tajuddin
author_sort	Siti Nur Syazni, Mohd Zuki
title	A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion
title_short	A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion
title_full	A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion
title_fullStr	A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion
title_full_unstemmed	A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion
title_sort	whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion
publisher	Elsevier Ltd
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/19852/ http://umpir.ump.edu.my/id/eprint/19852/ http://umpir.ump.edu.my/id/eprint/19852/ http://umpir.ump.edu.my/id/eprint/19852/1/fist-2018-nina-whole%20cell%20bio-optode%20based%20on%20immobilized%20nitrite-degrading.pdf
first_indexed	2023-09-18T22:28:27Z
last_indexed	2023-09-18T22:28:27Z
_version_	1777416124817735680

A whole cell bio-optode based on immobilized nitrite-degrading microorganism on the acrylic microspheres for visual quantitation of nitrite ion

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