Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1

Recombinant bromelain is a cysteine protease that can be exploited for its protease activity in food and pharmaceutical applications. The aim of this study was to compare different purification methods aqueous two-phase system (ATPS), ammonium sulphate precipitation,...

Full description

Bibliographic Details
Main Authors:	Zatul Iffah, Mohd Arshad, Azura, Amid, Faridah, Yusof, Siti Zubaidah, Sulaiman, Siti Kholijah, Abdul Mudalip, Rohaida, Che Man, Shalyda, Md Shaarani
Format:	Article
Language:	English
Published:	The Malaysian Analytical Sciences Society 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/19928/ http://umpir.ump.edu.my/id/eprint/19928/ http://umpir.ump.edu.my/id/eprint/19928/ http://umpir.ump.edu.my/id/eprint/19928/1/fkksa-2017-zatul-comparison%20of%20purification%20method.pdf

id	ump-19928
recordtype	eprints
spelling	ump-199282018-01-09T02:26:52Z http://umpir.ump.edu.my/id/eprint/19928/ Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1 Zatul Iffah, Mohd Arshad Azura, Amid Faridah, Yusof Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Rohaida, Che Man Shalyda, Md Shaarani TP Chemical technology Recombinant bromelain is a cysteine protease that can be exploited for its protease activity in food and pharmaceutical applications. The aim of this study was to compare different purification methods aqueous two-phase system (ATPS), ammonium sulphate precipitation, ion exchange, affinity, and gel filtration chromatography) for the recombinant bromelain purification from Escherichia coli BL21-A1. From the SDS-PAGE analysis, all methods produced band with molecular weight between 50 to 55 kDa. Among the methods used, the ATPS purification consisting of 13% (w/w) of PEG6000 and 11% (w/w) potassium phosphate at pH 7.0 was chosen as the best purification method. The method produced 16.39 ± 0.03% of yield, purification fold of 5.35 ± 0.11, and specific activity of 3.47 ± 0.11 unit/mg of recombinant bromelain. This proposed study can be used as a platform for large-scale downstream processing of recombinant bromelain in industry. The Malaysian Analytical Sciences Society 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19928/1/fkksa-2017-zatul-comparison%20of%20purification%20method.pdf Zatul Iffah, Mohd Arshad and Azura, Amid and Faridah, Yusof and Siti Zubaidah, Sulaiman and Siti Kholijah, Abdul Mudalip and Rohaida, Che Man and Shalyda, Md Shaarani (2017) Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1. Malaysian Journal of Analytical Sciences, 21 (4). pp. 958-971. ISSN 1394-2506 http://www.ukm.my/mjas/v21_n4/pdf/ZatulIffah_21_4_23.pdf DOI: https://do i.org/10.17576/mjas-2017-2104-23
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Zatul Iffah, Mohd Arshad Azura, Amid Faridah, Yusof Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Rohaida, Che Man Shalyda, Md Shaarani Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1
description	Recombinant bromelain is a cysteine protease that can be exploited for its protease activity in food and pharmaceutical applications. The aim of this study was to compare different purification methods aqueous two-phase system (ATPS), ammonium sulphate precipitation, ion exchange, affinity, and gel filtration chromatography) for the recombinant bromelain purification from Escherichia coli BL21-A1. From the SDS-PAGE analysis, all methods produced band with molecular weight between 50 to 55 kDa. Among the methods used, the ATPS purification consisting of 13% (w/w) of PEG6000 and 11% (w/w) potassium phosphate at pH 7.0 was chosen as the best purification method. The method produced 16.39 ± 0.03% of yield, purification fold of 5.35 ± 0.11, and specific activity of 3.47 ± 0.11 unit/mg of recombinant bromelain. This proposed study can be used as a platform for large-scale downstream processing of recombinant bromelain in industry.
format	Article
author	Zatul Iffah, Mohd Arshad Azura, Amid Faridah, Yusof Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Rohaida, Che Man Shalyda, Md Shaarani
author_facet	Zatul Iffah, Mohd Arshad Azura, Amid Faridah, Yusof Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Rohaida, Che Man Shalyda, Md Shaarani
author_sort	Zatul Iffah, Mohd Arshad
title	Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1
title_short	Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1
title_full	Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1
title_fullStr	Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1
title_full_unstemmed	Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1
title_sort	comparison of purification methods to purify recombinant bromelain from escherichia coli bl21-a1
publisher	The Malaysian Analytical Sciences Society
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/19928/ http://umpir.ump.edu.my/id/eprint/19928/ http://umpir.ump.edu.my/id/eprint/19928/ http://umpir.ump.edu.my/id/eprint/19928/1/fkksa-2017-zatul-comparison%20of%20purification%20method.pdf
first_indexed	2023-09-18T22:28:33Z
last_indexed	2023-09-18T22:28:33Z
_version_	1777416131241312256

Comparison of Purification Methods to Purify Recombinant Bromelain from Escherichia coli BL21-A1

Similar Items