Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6

Enzymatic hydrolysis becomes a prominent technology for conversion of cellulosic biomass to its glucose monomers that requires an action of cellulolytic enzymes in a sequential and synergistic manner. In this study, the effect of agitation speed, glucan loading, enzyme loading, temperature and react...

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Main Authors:	Fatin Syazwana, Hashim, Wan Mohd Hafizuddin, Wan Yussof, Mior Ahmad Khushairi, Mohd Zahari, Roshanida, A. Rahman, Rosli, Md. Illias
Format:	Conference or Workshop Item
Language:	English
Published:	IOP Publishing 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/19935/ http://umpir.ump.edu.my/id/eprint/19935/ http://umpir.ump.edu.my/id/eprint/19935/1/fkksa-2017-Fatin-Enzymatic%20Hydrolysis%20of%20Pretreated%20Fibre%20Pressed.pdf

id	ump-19935
recordtype	eprints
spelling	ump-199352018-01-09T04:18:34Z http://umpir.ump.edu.my/id/eprint/19935/ Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6 Fatin Syazwana, Hashim Wan Mohd Hafizuddin, Wan Yussof Mior Ahmad Khushairi, Mohd Zahari Roshanida, A. Rahman Rosli, Md. Illias TP Chemical technology Enzymatic hydrolysis becomes a prominent technology for conversion of cellulosic biomass to its glucose monomers that requires an action of cellulolytic enzymes in a sequential and synergistic manner. In this study, the effect of agitation speed, glucan loading, enzyme loading, temperature and reaction time on the production of glucose from fibre pressed oil palm frond (FPOPF) during enzymatic hydrolysis was screened by a half factorial design 25-1 using Response Surface Methodology (RSM). The FPOPF sample was first delignified by alkaline pretreatment at 4.42 (w/v) sodium hydroxide for an hour prior to enzymatic hydrolysis using commercial cellulase enzyme, Sacchariseb C6. The effect of enzymatic hydrolysis on the structural of FPOPF has been evaluated by Scanning Electron Microscopy (SEM) analysis. Characterization of raw FPOPF comprised of 4.5 extractives, 40.7 glucan, 26.1 xylan, 26.2 lignin and 1.8 ash, whereas for pretreated FPOPF gave 0.3 extractives, 61.4 glucan, 20.4 xylan, 13.3 lignin and 1.3 ash. From this study, it was found that the best enzymatic hydrolysis condition yielded 33.01 ± 0.73 g/L of glucose when performed at 200 rpm of agitation speed, 60 FPU/mL of enzyme loading, 4 (w/w) of glucan loading, temperature at 55 squ and 72 hours of reaction time. The model obtained was significant with p-value <0.0001 as verified by the analysis of variance (ANOVA). The coefficient of determination (R2) from ANOVA study was 0.9959. Overall, it can be concluded that addition of Sacchariseb C6 during enzymatic hydrolysis from pretreated FPOPF produce high amount of glucose that enhances it potential for industrial application. This glucose can be further used to produce high-value products. IOP Publishing 2017 Conference or Workshop Item PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/19935/1/fkksa-2017-Fatin-Enzymatic%20Hydrolysis%20of%20Pretreated%20Fibre%20Pressed.pdf Fatin Syazwana, Hashim and Wan Mohd Hafizuddin, Wan Yussof and Mior Ahmad Khushairi, Mohd Zahari and Roshanida, A. Rahman and Rosli, Md. Illias (2017) Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6. In: IOP Conference Series: Materials Science and Engineering, 29th Symposium of Malaysian Chemical Engineers (SOMChE) 2016, 1-3 December 2016 , Miri, Sarawak, Malaysia. pp. 1-8., 206 (012008). ISSN 1757-899X https://doi.org/10.1088/1757-899X/206/1/012008
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Fatin Syazwana, Hashim Wan Mohd Hafizuddin, Wan Yussof Mior Ahmad Khushairi, Mohd Zahari Roshanida, A. Rahman Rosli, Md. Illias Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6
description	Enzymatic hydrolysis becomes a prominent technology for conversion of cellulosic biomass to its glucose monomers that requires an action of cellulolytic enzymes in a sequential and synergistic manner. In this study, the effect of agitation speed, glucan loading, enzyme loading, temperature and reaction time on the production of glucose from fibre pressed oil palm frond (FPOPF) during enzymatic hydrolysis was screened by a half factorial design 25-1 using Response Surface Methodology (RSM). The FPOPF sample was first delignified by alkaline pretreatment at 4.42 (w/v) sodium hydroxide for an hour prior to enzymatic hydrolysis using commercial cellulase enzyme, Sacchariseb C6. The effect of enzymatic hydrolysis on the structural of FPOPF has been evaluated by Scanning Electron Microscopy (SEM) analysis. Characterization of raw FPOPF comprised of 4.5 extractives, 40.7 glucan, 26.1 xylan, 26.2 lignin and 1.8 ash, whereas for pretreated FPOPF gave 0.3 extractives, 61.4 glucan, 20.4 xylan, 13.3 lignin and 1.3 ash. From this study, it was found that the best enzymatic hydrolysis condition yielded 33.01 ± 0.73 g/L of glucose when performed at 200 rpm of agitation speed, 60 FPU/mL of enzyme loading, 4 (w/w) of glucan loading, temperature at 55 squ and 72 hours of reaction time. The model obtained was significant with p-value <0.0001 as verified by the analysis of variance (ANOVA). The coefficient of determination (R2) from ANOVA study was 0.9959. Overall, it can be concluded that addition of Sacchariseb C6 during enzymatic hydrolysis from pretreated FPOPF produce high amount of glucose that enhances it potential for industrial application. This glucose can be further used to produce high-value products.
format	Conference or Workshop Item
author	Fatin Syazwana, Hashim Wan Mohd Hafizuddin, Wan Yussof Mior Ahmad Khushairi, Mohd Zahari Roshanida, A. Rahman Rosli, Md. Illias
author_facet	Fatin Syazwana, Hashim Wan Mohd Hafizuddin, Wan Yussof Mior Ahmad Khushairi, Mohd Zahari Roshanida, A. Rahman Rosli, Md. Illias
author_sort	Fatin Syazwana, Hashim
title	Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6
title_short	Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6
title_full	Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6
title_fullStr	Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6
title_full_unstemmed	Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6
title_sort	enzymatic hydrolysis of pretreated fibre pressed oil palm frond by using sacchariseb c6
publisher	IOP Publishing
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/19935/ http://umpir.ump.edu.my/id/eprint/19935/ http://umpir.ump.edu.my/id/eprint/19935/1/fkksa-2017-Fatin-Enzymatic%20Hydrolysis%20of%20Pretreated%20Fibre%20Pressed.pdf
first_indexed	2023-09-18T22:28:34Z
last_indexed	2023-09-18T22:28:34Z
_version_	1777416131819077632

Enzymatic Hydrolysis of Pretreated Fibre Pressed Oil Palm Frond by using Sacchariseb C6

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