Biomolecular Aspects of Second Order Limit Language

The study on the recombinant behavior of double-stranded DNA molecules has led to the mathematical modelling of DNA splicing system. The interdisciplinary study is founded from the knowledge of informational macromolecules and formal language theory. A splicing language is resulted from a splicing s...

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Main Authors:	Muhammad Azrin, Ahmad, Nor Haniza, Sarmin, Mohd Firdaus, Abdul-Wahab, Fong, Wan Heng, Yuhani, Yusof
Format:	Article
Language:	English
Published:	Penerbit UTM Press 2018
Subjects:	QA Mathematics
Online Access:	http://umpir.ump.edu.my/id/eprint/20041/ http://umpir.ump.edu.my/id/eprint/20041/ http://umpir.ump.edu.my/id/eprint/20041/1/Biomolecular%20Aspects%20of%20Second%20Order%20Limit%20Language-fist-2018.pdf

id	ump-20041
recordtype	eprints
spelling	ump-200412018-07-18T08:33:01Z http://umpir.ump.edu.my/id/eprint/20041/ Biomolecular Aspects of Second Order Limit Language Muhammad Azrin, Ahmad Nor Haniza, Sarmin Mohd Firdaus, Abdul-Wahab Fong, Wan Heng Yuhani, Yusof QA Mathematics The study on the recombinant behavior of double-stranded DNA molecules has led to the mathematical modelling of DNA splicing system. The interdisciplinary study is founded from the knowledge of informational macromolecules and formal language theory. A splicing language is resulted from a splicing system, in which different types of splicing langauges have been researched previous, namely adult/inert, transcient and first order limit language. Recently, second order limit language has been extensively explored from the first order limit language. Therefore, in this paper, a laboratory experiment was conducted to validate the existence of a second order limit language. To accomplish it, an initial strand of double-stranded DNA, amplified from bacteriophage lambda, was generated through polymerase chain reaction to generate thousands of copies of double-stranded DNA molecules. A restriction enzyme and ligase were added to the solution to complete the reaction. The reaction mixture was then subjected to polyacrylamide gel electrophoresis to separate biological macromolecules according to their sizes. A mathematical model derived at the early study was used to predict the approximate length of each string in the splicing language. The results obtained from the experiment are then used to verify the mathematical model of a second order limit language. This study shows that the theory on the second order limit language is biologically proven hence the model has been validated. Penerbit UTM Press 2018 Article PeerReviewed application/pdf en cc_by_nc http://umpir.ump.edu.my/id/eprint/20041/1/Biomolecular%20Aspects%20of%20Second%20Order%20Limit%20Language-fist-2018.pdf Muhammad Azrin, Ahmad and Nor Haniza, Sarmin and Mohd Firdaus, Abdul-Wahab and Fong, Wan Heng and Yuhani, Yusof (2018) Biomolecular Aspects of Second Order Limit Language. Malaysian Journal of Fundamental and Applied Sciences, 14 (1). pp. 15-19. ISSN 2289-599X https://mjfas.utm.my/index.php/mjfas/article/view/727
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QA Mathematics
spellingShingle	QA Mathematics Muhammad Azrin, Ahmad Nor Haniza, Sarmin Mohd Firdaus, Abdul-Wahab Fong, Wan Heng Yuhani, Yusof Biomolecular Aspects of Second Order Limit Language
description	The study on the recombinant behavior of double-stranded DNA molecules has led to the mathematical modelling of DNA splicing system. The interdisciplinary study is founded from the knowledge of informational macromolecules and formal language theory. A splicing language is resulted from a splicing system, in which different types of splicing langauges have been researched previous, namely adult/inert, transcient and first order limit language. Recently, second order limit language has been extensively explored from the first order limit language. Therefore, in this paper, a laboratory experiment was conducted to validate the existence of a second order limit language. To accomplish it, an initial strand of double-stranded DNA, amplified from bacteriophage lambda, was generated through polymerase chain reaction to generate thousands of copies of double-stranded DNA molecules. A restriction enzyme and ligase were added to the solution to complete the reaction. The reaction mixture was then subjected to polyacrylamide gel electrophoresis to separate biological macromolecules according to their sizes. A mathematical model derived at the early study was used to predict the approximate length of each string in the splicing language. The results obtained from the experiment are then used to verify the mathematical model of a second order limit language. This study shows that the theory on the second order limit language is biologically proven hence the model has been validated.
format	Article
author	Muhammad Azrin, Ahmad Nor Haniza, Sarmin Mohd Firdaus, Abdul-Wahab Fong, Wan Heng Yuhani, Yusof
author_facet	Muhammad Azrin, Ahmad Nor Haniza, Sarmin Mohd Firdaus, Abdul-Wahab Fong, Wan Heng Yuhani, Yusof
author_sort	Muhammad Azrin, Ahmad
title	Biomolecular Aspects of Second Order Limit Language
title_short	Biomolecular Aspects of Second Order Limit Language
title_full	Biomolecular Aspects of Second Order Limit Language
title_fullStr	Biomolecular Aspects of Second Order Limit Language
title_full_unstemmed	Biomolecular Aspects of Second Order Limit Language
title_sort	biomolecular aspects of second order limit language
publisher	Penerbit UTM Press
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/20041/ http://umpir.ump.edu.my/id/eprint/20041/ http://umpir.ump.edu.my/id/eprint/20041/1/Biomolecular%20Aspects%20of%20Second%20Order%20Limit%20Language-fist-2018.pdf
first_indexed	2023-09-18T22:28:42Z
last_indexed	2023-09-18T22:28:42Z
_version_	1777416140429983744

Biomolecular Aspects of Second Order Limit Language

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