In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H.

The neuroprotective properties and antioxidant potential of polyphenolic extracts from a brown seaweed Padina australis, were evaluated by the inhibition assay against acetylcholine esterase using indoxyl acetate as the substrate, and by the radical scavenging activity assay against 1,1-diphenyl-2-p...

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Main Authors:	Murugan, Amarchand Chordia, Vallal, Dahineeswari, Karim, Md. Rezaul, Govindan, Natanamurugaraj, M. M., Yusoff, Rahman, Md. Mokhlesur
Format:	Article
Language:	English
Published:	JOCPR 2015
Subjects:	QK Botany
Online Access:	http://umpir.ump.edu.my/id/eprint/20543/ http://umpir.ump.edu.my/id/eprint/20543/ http://umpir.ump.edu.my/id/eprint/20543/1/Ama-paper-2.pdf

id	ump-20543
recordtype	eprints
spelling	ump-205432018-07-27T03:15:40Z http://umpir.ump.edu.my/id/eprint/20543/ In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H. Murugan, Amarchand Chordia Vallal, Dahineeswari Karim, Md. Rezaul Govindan, Natanamurugaraj M. M., Yusoff Rahman, Md. Mokhlesur QK Botany The neuroprotective properties and antioxidant potential of polyphenolic extracts from a brown seaweed Padina australis, were evaluated by the inhibition assay against acetylcholine esterase using indoxyl acetate as the substrate, and by the radical scavenging activity assay against 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and ferrous ion chelating (FIC) activity assay. Methanolic (80%) crude extracts was fractionated by different solvent partitioning, and the assays of each fraction were performed using the microplate reader. The highest total phenolic content (TPC) was observed in n-butanol soluble fraction with a value of 188.75±0.21 mg GAE/g. In addition, the n-butanol soluble fraction showed the highest ferric reducing power (188.34±0.15 mg GAE/g) and the highest ferrous ion chelating ability (76.52±0.12%). The highest free radical scavenging activity against DPPH was found in water soluble fraction with 77.5±1.82% inhibition. However, the βcarotene bleaching activity was found higher with the non-polar solvent, n-hexane extract (58.12±0.58%). A strong positive correlation was found between the total phenolics and antioxidant activities of the fractions. Acetylcholinesterase (AChE) inhibitory properties of all the fractions were assayed where n-butanol soluble fraction was found to have better AChE inhibitory activity (57.94±0.17%) with an IC50 value of 1.54±0.045 mg/ml. The results suggested that the polar fractions possess higher AChE inhibitory and antioxidant activity. It can be concluded that Padina australis possesses an appreciable amount of polyphenols with notable antioxidant and antiAChE properties that could be promisingly used in the treatment of neurodegenerative disorders. JOCPR 2015-09-14 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/20543/1/Ama-paper-2.pdf Murugan, Amarchand Chordia and Vallal, Dahineeswari and Karim, Md. Rezaul and Govindan, Natanamurugaraj and M. M., Yusoff and Rahman, Md. Mokhlesur (2015) In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H. Journal of Chemical and Pharmaceutical Research, 7 (8). pp. 355-362. ISSN 0975-7384 http://www.jocpr.com/abstract/in-vitro-antiradical-and-neuroprotective-activity-of-polyphenolic-extract-from-marine-algae-padina-australis-h-5275.html
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QK Botany
spellingShingle	QK Botany Murugan, Amarchand Chordia Vallal, Dahineeswari Karim, Md. Rezaul Govindan, Natanamurugaraj M. M., Yusoff Rahman, Md. Mokhlesur In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H.
description	The neuroprotective properties and antioxidant potential of polyphenolic extracts from a brown seaweed Padina australis, were evaluated by the inhibition assay against acetylcholine esterase using indoxyl acetate as the substrate, and by the radical scavenging activity assay against 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), and ferrous ion chelating (FIC) activity assay. Methanolic (80%) crude extracts was fractionated by different solvent partitioning, and the assays of each fraction were performed using the microplate reader. The highest total phenolic content (TPC) was observed in n-butanol soluble fraction with a value of 188.75±0.21 mg GAE/g. In addition, the n-butanol soluble fraction showed the highest ferric reducing power (188.34±0.15 mg GAE/g) and the highest ferrous ion chelating ability (76.52±0.12%). The highest free radical scavenging activity against DPPH was found in water soluble fraction with 77.5±1.82% inhibition. However, the βcarotene bleaching activity was found higher with the non-polar solvent, n-hexane extract (58.12±0.58%). A strong positive correlation was found between the total phenolics and antioxidant activities of the fractions. Acetylcholinesterase (AChE) inhibitory properties of all the fractions were assayed where n-butanol soluble fraction was found to have better AChE inhibitory activity (57.94±0.17%) with an IC50 value of 1.54±0.045 mg/ml. The results suggested that the polar fractions possess higher AChE inhibitory and antioxidant activity. It can be concluded that Padina australis possesses an appreciable amount of polyphenols with notable antioxidant and antiAChE properties that could be promisingly used in the treatment of neurodegenerative disorders.
format	Article
author	Murugan, Amarchand Chordia Vallal, Dahineeswari Karim, Md. Rezaul Govindan, Natanamurugaraj M. M., Yusoff Rahman, Md. Mokhlesur
author_facet	Murugan, Amarchand Chordia Vallal, Dahineeswari Karim, Md. Rezaul Govindan, Natanamurugaraj M. M., Yusoff Rahman, Md. Mokhlesur
author_sort	Murugan, Amarchand Chordia
title	In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H.
title_short	In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H.
title_full	In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H.
title_fullStr	In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H.
title_full_unstemmed	In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H.
title_sort	in vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae padina australis h.
publisher	JOCPR
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/20543/ http://umpir.ump.edu.my/id/eprint/20543/ http://umpir.ump.edu.my/id/eprint/20543/1/Ama-paper-2.pdf
first_indexed	2023-09-18T22:29:41Z
last_indexed	2023-09-18T22:29:41Z
_version_	1777416202385096704

In vitro antiradical and neuroprotective activity of polyphenolic extract from marine algae Padina australis H.

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