Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria

Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria

Objectives: To make preliminary screening study for the presence of ESBL producing bacteria, in food and drinks sold in Gombe metropolis. 30 samples of food and drinks were collected and analyzed. Method: Bacterial isolation was performed on nutrient agar and identification started from gram stainin...

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Main Authors: Shu’aibu, I., Hadiza, J. A., Yusha’u, M., Kabiru, M. Y., Ahmad, Mani Malam, Lawal, G., Adamu, M. T., Khairiyya, M.
Format: Article
Language:English
Published: Informatics Publishing Limited 2016
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/21408/
http://umpir.ump.edu.my/id/eprint/21408/
http://umpir.ump.edu.my/id/eprint/21408/
http://umpir.ump.edu.my/id/eprint/21408/1/Assessment%20of%20Foods%20and%20Drinks%20for%20the%20Presence.pdf
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spelling ump-214082018-06-27T08:12:51Z http://umpir.ump.edu.my/id/eprint/21408/ Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria Shu’aibu, I. Hadiza, J. A. Yusha’u, M. Kabiru, M. Y. Ahmad, Mani Malam Lawal, G. Adamu, M. T. Khairiyya, M. TP Chemical technology Objectives: To make preliminary screening study for the presence of ESBL producing bacteria, in food and drinks sold in Gombe metropolis. 30 samples of food and drinks were collected and analyzed. Method: Bacterial isolation was performed on nutrient agar and identification started from gram staining; the gram negative isolates obtained, among which ESBL producers were found and further subjected to standard biochemical tests and confirmed to be enterobacteriaceae. They were then screened for ESBLs in accordance with Clinical and Laboratory Standard Institute (CLSI) breakpoint, and the suspected ESBLs bacteria were confirmed using double disc synergy testing (DDST) with standard Augmentin (Amoxycillin/ Clavulanic Acid, 30μg; Oxoid England, CT0223B), Ceftriaxone (CRO 30μg; Oxoid England, CT0417B) and Ceftazidime (CAZ 30μg; Oxoid England, CT0412B). Findings: The fourteen 14(100%) Gram negative bacteria isolated were found to include Pseudomonasaeruginosa 2(14.29%) and members of the family Enterobacteriaceae (enteric gram negative rods): Citrobacterfreundii 2(14.29%), Enterobacterspp. 1(7.14%), Escherichiacoli 4(28.57%), Klebsiellapneumoniae 3(21.43%), Proteusvulgaris 1(7.14%) and Salmonellatyphi 1(7.14%). When screened for ESBL production based on CLSI breakpoint, only 6 (49.99%) were positive and included C.freundii 1(7.14%), E.coli 2(14.29%), K.pneumoniae 1(7.14%), S. typhi 1(7.14%) and 1(7.14%) for Ps. aeruginosa. On subjecting them to DDST, only 2(33.34%) out of them were found to be ESBLs positive by an increase in inhibition zone of 5mm toward the centre disc, Augmentin. They include E. coli 1(16.67%) and S. typhi 1(16.67%). Application: In a nutshell, foods and drinks sold therein could serve as reservoirs for ESBLs producing bacteria. Informatics Publishing Limited 2016 Article PeerReviewed text en cc_by http://umpir.ump.edu.my/id/eprint/21408/1/Assessment%20of%20Foods%20and%20Drinks%20for%20the%20Presence.pdf Shu’aibu, I. and Hadiza, J. A. and Yusha’u, M. and Kabiru, M. Y. and Ahmad, Mani Malam and Lawal, G. and Adamu, M. T. and Khairiyya, M. (2016) Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria. Indian Journal of Science and Technology, 9 (48). pp. 1-9. ISSN 0974-6846 (Print); 0974-5645 (Online) http://dx.doi.org/10.17485/ijst%2F2016%2Fv9i48%2F109624 DOI: 10.17485/ijst/2016/v9i48/109624
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Shu’aibu, I.
Hadiza, J. A.
Yusha’u, M.
Kabiru, M. Y.
Ahmad, Mani Malam
Lawal, G.
Adamu, M. T.
Khairiyya, M.
Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria
description Objectives: To make preliminary screening study for the presence of ESBL producing bacteria, in food and drinks sold in Gombe metropolis. 30 samples of food and drinks were collected and analyzed. Method: Bacterial isolation was performed on nutrient agar and identification started from gram staining; the gram negative isolates obtained, among which ESBL producers were found and further subjected to standard biochemical tests and confirmed to be enterobacteriaceae. They were then screened for ESBLs in accordance with Clinical and Laboratory Standard Institute (CLSI) breakpoint, and the suspected ESBLs bacteria were confirmed using double disc synergy testing (DDST) with standard Augmentin (Amoxycillin/ Clavulanic Acid, 30μg; Oxoid England, CT0223B), Ceftriaxone (CRO 30μg; Oxoid England, CT0417B) and Ceftazidime (CAZ 30μg; Oxoid England, CT0412B). Findings: The fourteen 14(100%) Gram negative bacteria isolated were found to include Pseudomonasaeruginosa 2(14.29%) and members of the family Enterobacteriaceae (enteric gram negative rods): Citrobacterfreundii 2(14.29%), Enterobacterspp. 1(7.14%), Escherichiacoli 4(28.57%), Klebsiellapneumoniae 3(21.43%), Proteusvulgaris 1(7.14%) and Salmonellatyphi 1(7.14%). When screened for ESBL production based on CLSI breakpoint, only 6 (49.99%) were positive and included C.freundii 1(7.14%), E.coli 2(14.29%), K.pneumoniae 1(7.14%), S. typhi 1(7.14%) and 1(7.14%) for Ps. aeruginosa. On subjecting them to DDST, only 2(33.34%) out of them were found to be ESBLs positive by an increase in inhibition zone of 5mm toward the centre disc, Augmentin. They include E. coli 1(16.67%) and S. typhi 1(16.67%). Application: In a nutshell, foods and drinks sold therein could serve as reservoirs for ESBLs producing bacteria.
format Article
author Shu’aibu, I.
Hadiza, J. A.
Yusha’u, M.
Kabiru, M. Y.
Ahmad, Mani Malam
Lawal, G.
Adamu, M. T.
Khairiyya, M.
author_facet Shu’aibu, I.
Hadiza, J. A.
Yusha’u, M.
Kabiru, M. Y.
Ahmad, Mani Malam
Lawal, G.
Adamu, M. T.
Khairiyya, M.
author_sort Shu’aibu, I.
title Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria
title_short Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria
title_full Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria
title_fullStr Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria
title_full_unstemmed Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria
title_sort assessment of foods and drinks for the presence of extended spectrum beta lactamase (esbl) producing bacteria in gombe metropolis, nigeria
publisher Informatics Publishing Limited
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/21408/
http://umpir.ump.edu.my/id/eprint/21408/
http://umpir.ump.edu.my/id/eprint/21408/
http://umpir.ump.edu.my/id/eprint/21408/1/Assessment%20of%20Foods%20and%20Drinks%20for%20the%20Presence.pdf
first_indexed 2023-09-18T22:31:23Z
last_indexed 2023-09-18T22:31:23Z
_version_ 1777416309471969280

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