Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique

Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique

Diabetes mellitus is a metabolic disorder disease emanating from the inability of body’s response to high glucose levels in the blood. However, the side-effects from consuming synthetic drugs have reduced the effectiveness, thus, the potential of purple fleabane (Vernonia cinerea) leaf was investiga...

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Main Authors: Alara, Oluwaseun Ruth, Nour, A. H., Ukaegbu, Chinonso Ishmael, Azhari, H. Nour
Format: Article
Language:English
Published: Elsevier Ltd 2018
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/21930/
http://umpir.ump.edu.my/id/eprint/21930/
http://umpir.ump.edu.my/id/eprint/21930/
http://umpir.ump.edu.my/id/eprint/21930/1/Vernonia%20cinerea%20leaves%20as%20the%20source%20of%20phenolic%20compounds%2C%20antioxidants.pdf
id ump-21930
recordtype eprints
spelling ump-219302020-02-28T07:59:14Z http://umpir.ump.edu.my/id/eprint/21930/ Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique Alara, Oluwaseun Ruth Nour, A. H. Ukaegbu, Chinonso Ishmael Azhari, H. Nour TP Chemical technology Diabetes mellitus is a metabolic disorder disease emanating from the inability of body’s response to high glucose levels in the blood. However, the side-effects from consuming synthetic drugs have reduced the effectiveness, thus, the potential of purple fleabane (Vernonia cinerea) leaf was investigated using microwave-assisted extraction (MAE) in this study. The influences of extraction factors such as irradiation time, microwave power level, feed/solvent, and ethanol concentration on the recovery yields of polyphenol and flavonoids were as well studied. Thirty experimental runs using a face-centered central composite design (FCCCD) with six centre points were employed and optimal yields were obtained by generating quadratic models based on response surface methodology. The obtained optimal MAE conditions were as follows: irradiation time, 2 min; microwave power level, 444 W; feed/solvent, 1:14 g/mL; and ethanol concentration, 47% v/v. There was an insignificant difference between the predicted and experimental data (p > 0.05), showing good predictive models for the extraction of phenolic compounds from V. cinerea leaves using MAE. Also, the results confirmed that MAE can recover higher phenolic compounds, antioxidant, and anti-diabetic activities from V. cinerea leaves in shorter extraction time. In addition, the phenolic compounds in V. cinerea leaf extract were identified using liquid chromatography-mass spectrometry quadrupole time of flight (LC–MS-Q-TOF) and Fourier transform infrared spectroscopy (FTIR). The extracts showed higher anti-diabetic activity using alpha-amylase and alpha-glycosidase assays which suggested its exploration in pharmaceutical and functional food industries Elsevier Ltd 2018-06-08 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/21930/1/Vernonia%20cinerea%20leaves%20as%20the%20source%20of%20phenolic%20compounds%2C%20antioxidants.pdf Alara, Oluwaseun Ruth and Nour, A. H. and Ukaegbu, Chinonso Ishmael and Azhari, H. Nour (2018) Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique. Industrial Crops and Products, 122. pp. 533-544. ISSN 0926-6690 https://doi.org/10.1016/j.indcrop.2018.06.034 https://doi.org/10.1016/j.indcrop.2018.06.034
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Alara, Oluwaseun Ruth
Nour, A. H.
Ukaegbu, Chinonso Ishmael
Azhari, H. Nour
Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique
description Diabetes mellitus is a metabolic disorder disease emanating from the inability of body’s response to high glucose levels in the blood. However, the side-effects from consuming synthetic drugs have reduced the effectiveness, thus, the potential of purple fleabane (Vernonia cinerea) leaf was investigated using microwave-assisted extraction (MAE) in this study. The influences of extraction factors such as irradiation time, microwave power level, feed/solvent, and ethanol concentration on the recovery yields of polyphenol and flavonoids were as well studied. Thirty experimental runs using a face-centered central composite design (FCCCD) with six centre points were employed and optimal yields were obtained by generating quadratic models based on response surface methodology. The obtained optimal MAE conditions were as follows: irradiation time, 2 min; microwave power level, 444 W; feed/solvent, 1:14 g/mL; and ethanol concentration, 47% v/v. There was an insignificant difference between the predicted and experimental data (p > 0.05), showing good predictive models for the extraction of phenolic compounds from V. cinerea leaves using MAE. Also, the results confirmed that MAE can recover higher phenolic compounds, antioxidant, and anti-diabetic activities from V. cinerea leaves in shorter extraction time. In addition, the phenolic compounds in V. cinerea leaf extract were identified using liquid chromatography-mass spectrometry quadrupole time of flight (LC–MS-Q-TOF) and Fourier transform infrared spectroscopy (FTIR). The extracts showed higher anti-diabetic activity using alpha-amylase and alpha-glycosidase assays which suggested its exploration in pharmaceutical and functional food industries
format Article
author Alara, Oluwaseun Ruth
Nour, A. H.
Ukaegbu, Chinonso Ishmael
Azhari, H. Nour
author_facet Alara, Oluwaseun Ruth
Nour, A. H.
Ukaegbu, Chinonso Ishmael
Azhari, H. Nour
author_sort Alara, Oluwaseun Ruth
title Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique
title_short Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique
title_full Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique
title_fullStr Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique
title_full_unstemmed Vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique
title_sort vernonia cinerea leaves as the source of phenolic compounds, antioxidants, and anti-diabetic activity using microwave-assisted extraction technique
publisher Elsevier Ltd
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/21930/
http://umpir.ump.edu.my/id/eprint/21930/
http://umpir.ump.edu.my/id/eprint/21930/
http://umpir.ump.edu.my/id/eprint/21930/1/Vernonia%20cinerea%20leaves%20as%20the%20source%20of%20phenolic%20compounds%2C%20antioxidants.pdf
first_indexed 2023-09-18T22:32:24Z
last_indexed 2023-09-18T22:32:24Z
_version_ 1777416373240070144

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