Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa

Background: Pseudomonas aeruginosa has emerged as a significant opportunistic bacterial pathogen that causes nosocomial infections in healthcare settings resulting in treatment failure throughout the world. This study was carried out to compare the relatedness between virulence characteristics and β...

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Main Authors:	M. K. K., Fazlul, A., Najnin, Farzana, Yasmin, M. A., Rashid, S., Deepthi, C., Srikumar, Rashid, Shah Samiur, M. H. M., Nazmul
Format:	Article
Language:	English
Published:	JIPR 2018
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/22918/ http://umpir.ump.edu.my/id/eprint/22918/ http://umpir.ump.edu.my/id/eprint/22918/1/Detection%20of%20virulence%20factors%20and%20%CE%B2%20lactamase%20encoding%20genes1.pdf

id	ump-22918
recordtype	eprints
spelling	ump-229182018-11-26T08:31:38Z http://umpir.ump.edu.my/id/eprint/22918/ Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa M. K. K., Fazlul A., Najnin Farzana, Yasmin M. A., Rashid S., Deepthi C., Srikumar Rashid, Shah Samiur M. H. M., Nazmul Q Science (General) Background: Pseudomonas aeruginosa has emerged as a significant opportunistic bacterial pathogen that causes nosocomial infections in healthcare settings resulting in treatment failure throughout the world. This study was carried out to compare the relatedness between virulence characteristics and β-lactamase encoding genes producing Pseudomonas aeruginosa. Methods: A total of 120 P. aeruginosa isolates were obtained from both paediatric and adult patients of Selayang Hospital, Kuala Lumpur, Malaysia. Phenotypic methods were used to detect various virulence factors (Phospholipase, Hemolysin, Gelatinase, DNAse, and Biofilm). All the isolates were evaluated for production of extended spectrum beta-lactamase (ESBL) as well as metallo β-lactamase (MBL) by Double-disk synergy test (DDST) and E-test while AmpC β-lactamase production was detected by disk antagonism test. JIPR 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22918/1/Detection%20of%20virulence%20factors%20and%20%CE%B2%20lactamase%20encoding%20genes1.pdf M. K. K., Fazlul and A., Najnin and Farzana, Yasmin and M. A., Rashid and S., Deepthi and C., Srikumar and Rashid, Shah Samiur and M. H. M., Nazmul (2018) Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa. Journal of International Pharmaceutical Research, 45. pp. 190-202. ISSN 1674-0440 https://www.scopus.com/record/display.uri?eid=2-s2.0-85056774631&origin=resultslist&sort=plf-f&src=s&st1=Detection+of+virulence+factors+and+%ce%b2+lactamase+encoding+genes+among+the+clinical+isolates+of+Pseudomonas+aeruginosa&st2=&sid=2b0b8f9ced731e1515299b756108f931&sot=b&sdt=b&sl=130&s=TITLE-ABS-KEY%28Detection+of+virulence+factors+and+%ce%b2+lactamase+encoding+genes+among+the+clinical+isolates+of+Pseudomonas+aeruginosa%29&relpos=1&citeCnt=0&searchTerm=
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) M. K. K., Fazlul A., Najnin Farzana, Yasmin M. A., Rashid S., Deepthi C., Srikumar Rashid, Shah Samiur M. H. M., Nazmul Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa
description	Background: Pseudomonas aeruginosa has emerged as a significant opportunistic bacterial pathogen that causes nosocomial infections in healthcare settings resulting in treatment failure throughout the world. This study was carried out to compare the relatedness between virulence characteristics and β-lactamase encoding genes producing Pseudomonas aeruginosa. Methods: A total of 120 P. aeruginosa isolates were obtained from both paediatric and adult patients of Selayang Hospital, Kuala Lumpur, Malaysia. Phenotypic methods were used to detect various virulence factors (Phospholipase, Hemolysin, Gelatinase, DNAse, and Biofilm). All the isolates were evaluated for production of extended spectrum beta-lactamase (ESBL) as well as metallo β-lactamase (MBL) by Double-disk synergy test (DDST) and E-test while AmpC β-lactamase production was detected by disk antagonism test.
format	Article
author	M. K. K., Fazlul A., Najnin Farzana, Yasmin M. A., Rashid S., Deepthi C., Srikumar Rashid, Shah Samiur M. H. M., Nazmul
author_facet	M. K. K., Fazlul A., Najnin Farzana, Yasmin M. A., Rashid S., Deepthi C., Srikumar Rashid, Shah Samiur M. H. M., Nazmul
author_sort	M. K. K., Fazlul
title	Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa
title_short	Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa
title_full	Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa
title_fullStr	Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa
title_full_unstemmed	Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa
title_sort	detection of virulence factors and β lactamase encoding genes among the clinical isolates of pseudomonas aeruginosa
publisher	JIPR
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/22918/ http://umpir.ump.edu.my/id/eprint/22918/ http://umpir.ump.edu.my/id/eprint/22918/1/Detection%20of%20virulence%20factors%20and%20%CE%B2%20lactamase%20encoding%20genes1.pdf
first_indexed	2023-09-18T22:34:06Z
last_indexed	2023-09-18T22:34:06Z
_version_	1777416479474450432

Detection of virulence factors and β lactamase encoding genes among the clinical isolates of Pseudomonas aeruginosa

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