Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin

A new biosensor for the analysis of nitrite in food was developed based on hemoglobin (Hb) covalently immobilized on the succinimide functionalized poly(n-butyl acrylate)-graphene [poly(nBA)-rGO] composite film deposited on a carbon-paste screen-printed electrode (SPE). The immobilized Hb on the pol...

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Main Authors:	Raja Zaidatul Akhmar, Raja Jamaluddin, Heng, Lee Yook, Tan, Ling Ling, K. F., Chong
Format:	Article
Language:	English English
Published:	MDPI 2018
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/23075/ http://umpir.ump.edu.my/id/eprint/23075/ http://umpir.ump.edu.my/id/eprint/23075/ http://umpir.ump.edu.my/id/eprint/23075/1/Electrochemical%20biosensor%20for%20nitrite%20based%20on%20polyacrylic-graphene%20composite%20film%20with%20covalently%20immobilized%20hemoglobin.pdf http://umpir.ump.edu.my/id/eprint/23075/7/Electrochemical%20Biosensor%20for%20Nitrite%20Based%20on%20Polyacrylic-Graphene%20Composite%20Film%20with%20Covalently%20Immobilized%20Hemoglobin.pdf

id	ump-23075
recordtype	eprints
spelling	ump-230752019-01-15T04:47:40Z http://umpir.ump.edu.my/id/eprint/23075/ Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin Raja Zaidatul Akhmar, Raja Jamaluddin Heng, Lee Yook Tan, Ling Ling K. F., Chong Q Science (General) A new biosensor for the analysis of nitrite in food was developed based on hemoglobin (Hb) covalently immobilized on the succinimide functionalized poly(n-butyl acrylate)-graphene [poly(nBA)-rGO] composite film deposited on a carbon-paste screen-printed electrode (SPE). The immobilized Hb on the poly(nBA)-rGO conducting matrix exhibited electrocatalytic ability for the reduction of nitrite with significant enhancement in the reduction peak at −0.6 V versus Ag/AgCl reference electrode. Thus, direct determination of nitrite can be achieved by monitoring the cathodic peak current signal of the proposed polyacrylic-graphene hybrid film-based voltammetric nitrite biosensor. The nitrite biosensor exhibited a reproducible dynamic linear response range from 0.05–5 mg L−1 nitrite and a detection limit of 0.03 mg L−1. No significant interference was observed by potential interfering ions such as Ca2+, Na+, K+, NH4+, Mg2+, and NO3− ions. Analysis of nitrite in both raw and processed edible bird’s nest (EBN) samples demonstrated recovery of close to 100%. The covalent immobilization of Hb on poly(nBA)-rGO composite film has improved the performance of the electrochemical nitrite biosensor in terms of broader detection range, lower detection limit, and prolonged biosensor stability. MDPI 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23075/1/Electrochemical%20biosensor%20for%20nitrite%20based%20on%20polyacrylic-graphene%20composite%20film%20with%20covalently%20immobilized%20hemoglobin.pdf pdf en http://umpir.ump.edu.my/id/eprint/23075/7/Electrochemical%20Biosensor%20for%20Nitrite%20Based%20on%20Polyacrylic-Graphene%20Composite%20Film%20with%20Covalently%20Immobilized%20Hemoglobin.pdf Raja Zaidatul Akhmar, Raja Jamaluddin and Heng, Lee Yook and Tan, Ling Ling and K. F., Chong (2018) Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin. Sensors, 18 (5). pp. 1-17. ISSN 1424-8220 https://doi.org/10.3390/s18051343 https://doi.org/10.3390/s18051343
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Raja Zaidatul Akhmar, Raja Jamaluddin Heng, Lee Yook Tan, Ling Ling K. F., Chong Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin
description	A new biosensor for the analysis of nitrite in food was developed based on hemoglobin (Hb) covalently immobilized on the succinimide functionalized poly(n-butyl acrylate)-graphene [poly(nBA)-rGO] composite film deposited on a carbon-paste screen-printed electrode (SPE). The immobilized Hb on the poly(nBA)-rGO conducting matrix exhibited electrocatalytic ability for the reduction of nitrite with significant enhancement in the reduction peak at −0.6 V versus Ag/AgCl reference electrode. Thus, direct determination of nitrite can be achieved by monitoring the cathodic peak current signal of the proposed polyacrylic-graphene hybrid film-based voltammetric nitrite biosensor. The nitrite biosensor exhibited a reproducible dynamic linear response range from 0.05–5 mg L−1 nitrite and a detection limit of 0.03 mg L−1. No significant interference was observed by potential interfering ions such as Ca2+, Na+, K+, NH4+, Mg2+, and NO3− ions. Analysis of nitrite in both raw and processed edible bird’s nest (EBN) samples demonstrated recovery of close to 100%. The covalent immobilization of Hb on poly(nBA)-rGO composite film has improved the performance of the electrochemical nitrite biosensor in terms of broader detection range, lower detection limit, and prolonged biosensor stability.
format	Article
author	Raja Zaidatul Akhmar, Raja Jamaluddin Heng, Lee Yook Tan, Ling Ling K. F., Chong
author_facet	Raja Zaidatul Akhmar, Raja Jamaluddin Heng, Lee Yook Tan, Ling Ling K. F., Chong
author_sort	Raja Zaidatul Akhmar, Raja Jamaluddin
title	Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin
title_short	Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin
title_full	Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin
title_fullStr	Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin
title_full_unstemmed	Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin
title_sort	electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin
publisher	MDPI
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/23075/ http://umpir.ump.edu.my/id/eprint/23075/ http://umpir.ump.edu.my/id/eprint/23075/ http://umpir.ump.edu.my/id/eprint/23075/1/Electrochemical%20biosensor%20for%20nitrite%20based%20on%20polyacrylic-graphene%20composite%20film%20with%20covalently%20immobilized%20hemoglobin.pdf http://umpir.ump.edu.my/id/eprint/23075/7/Electrochemical%20Biosensor%20for%20Nitrite%20Based%20on%20Polyacrylic-Graphene%20Composite%20Film%20with%20Covalently%20Immobilized%20Hemoglobin.pdf
first_indexed	2023-09-18T22:34:26Z
last_indexed	2023-09-18T22:34:26Z
_version_	1777416500810874880

Electrochemical biosensor for nitrite based on polyacrylic-graphene composite film with covalently immobilized hemoglobin

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