A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide

An electrochemical DNA biosensor for DNA determination of genetically modified (GM) soybean (CaMV 35S target genes) was developed utilizing a new detection concept based on the adsoption of anthraquinone‐2‐sulphonic acid (AQMS) on the reduced graphene oxide nano‐particles (rGO) during DNA hybridizat...

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Main Authors:	Raja Zaidatul Akhmar, Raja Jamaluddin, Lee, Yook Heng, Ling, Ling Tan, K. F., Chong
Format:	Article
Language:	English
Published:	WILEY-VCH Verlag 2018
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/23789/ http://umpir.ump.edu.my/id/eprint/23789/ http://umpir.ump.edu.my/id/eprint/23789/ http://umpir.ump.edu.my/id/eprint/23789/1/A%20Biosensor%20for%20Genetic%20Modified%20Soybean%20DNA%20Determination%20via%20Adsorption%20of%20Anthraquinone%E2%80%902%E2%80%90sulphonic%20Acid%20in%20Reduced%20Graphene%20Oxide1.pdf

id	ump-23789
recordtype	eprints
spelling	ump-237892019-01-15T04:42:10Z http://umpir.ump.edu.my/id/eprint/23789/ A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide Raja Zaidatul Akhmar, Raja Jamaluddin Lee, Yook Heng Ling, Ling Tan K. F., Chong QD Chemistry An electrochemical DNA biosensor for DNA determination of genetically modified (GM) soybean (CaMV 35S target genes) was developed utilizing a new detection concept based on the adsoption of anthraquinone‐2‐sulphonic acid (AQMS) on the reduced graphene oxide nano‐particles (rGO) during DNA hybridization events. The aminated DNA probe for CaMV 35S was immobilized onto poly(n‐butyl acrylate) film modified with succinimide functional groups [poly(nBA‐NAS)] via peptide covalent bond. Nanosheets of rGO were entrapped in the poly(nBA‐NAS) film to form a conducting [poly(nBA‐NAS)‐rGO] film of the DNA biosensor. Besides facilitating the electron transfer reactions, the rGO also functioned as an adsorbent for AQMS. The sensing mechanism of the proposed DNA biosensor involved measuring the oxidation current of the AQMS adsorbed on the electrode surface at −0.50 V using differential pulse voltammetry (DPV) before and after a DNA hybridization event. Under optimum conditions, the DNA biosensor demonstrated a linear proportionality between AQMS oxidation signal and logarithm cDNA concentration from 1.0×10−15 M to 1.0×10−8 M target DNA with a detection limit of 6.3×10−16 M. The electrochemical DNA biosensor possessed good selectivity and a shelf life of about 40 days with relative standard deviation of reproducibility obtained in the range of 3.7–4.6% (n=5). Evaluation of the DNA biosensor using GM soybean DNA extracts showed excellent recovery percentages of 97.2–104.0. WILEY-VCH Verlag 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23789/1/A%20Biosensor%20for%20Genetic%20Modified%20Soybean%20DNA%20Determination%20via%20Adsorption%20of%20Anthraquinone%E2%80%902%E2%80%90sulphonic%20Acid%20in%20Reduced%20Graphene%20Oxide1.pdf Raja Zaidatul Akhmar, Raja Jamaluddin and Lee, Yook Heng and Ling, Ling Tan and K. F., Chong (2018) A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide. Electroanalysis, 30 (2). pp. 250-258. ISSN 1521-4109 https://doi.org/10.1002/elan.201700612 https://doi.org/10.1002/elan.201700612
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Raja Zaidatul Akhmar, Raja Jamaluddin Lee, Yook Heng Ling, Ling Tan K. F., Chong A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide
description	An electrochemical DNA biosensor for DNA determination of genetically modified (GM) soybean (CaMV 35S target genes) was developed utilizing a new detection concept based on the adsoption of anthraquinone‐2‐sulphonic acid (AQMS) on the reduced graphene oxide nano‐particles (rGO) during DNA hybridization events. The aminated DNA probe for CaMV 35S was immobilized onto poly(n‐butyl acrylate) film modified with succinimide functional groups [poly(nBA‐NAS)] via peptide covalent bond. Nanosheets of rGO were entrapped in the poly(nBA‐NAS) film to form a conducting [poly(nBA‐NAS)‐rGO] film of the DNA biosensor. Besides facilitating the electron transfer reactions, the rGO also functioned as an adsorbent for AQMS. The sensing mechanism of the proposed DNA biosensor involved measuring the oxidation current of the AQMS adsorbed on the electrode surface at −0.50 V using differential pulse voltammetry (DPV) before and after a DNA hybridization event. Under optimum conditions, the DNA biosensor demonstrated a linear proportionality between AQMS oxidation signal and logarithm cDNA concentration from 1.0×10−15 M to 1.0×10−8 M target DNA with a detection limit of 6.3×10−16 M. The electrochemical DNA biosensor possessed good selectivity and a shelf life of about 40 days with relative standard deviation of reproducibility obtained in the range of 3.7–4.6% (n=5). Evaluation of the DNA biosensor using GM soybean DNA extracts showed excellent recovery percentages of 97.2–104.0.
format	Article
author	Raja Zaidatul Akhmar, Raja Jamaluddin Lee, Yook Heng Ling, Ling Tan K. F., Chong
author_facet	Raja Zaidatul Akhmar, Raja Jamaluddin Lee, Yook Heng Ling, Ling Tan K. F., Chong
author_sort	Raja Zaidatul Akhmar, Raja Jamaluddin
title	A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide
title_short	A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide
title_full	A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide
title_fullStr	A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide
title_full_unstemmed	A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide
title_sort	biosensor for genetic modified soybean dna determination via adsorption of anthraquinone‐2‐sulphonic acid in reduced graphene oxide
publisher	WILEY-VCH Verlag
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/23789/ http://umpir.ump.edu.my/id/eprint/23789/ http://umpir.ump.edu.my/id/eprint/23789/ http://umpir.ump.edu.my/id/eprint/23789/1/A%20Biosensor%20for%20Genetic%20Modified%20Soybean%20DNA%20Determination%20via%20Adsorption%20of%20Anthraquinone%E2%80%902%E2%80%90sulphonic%20Acid%20in%20Reduced%20Graphene%20Oxide1.pdf
first_indexed	2023-09-18T22:35:47Z
last_indexed	2023-09-18T22:35:47Z
_version_	1777416586265624576

A Biosensor for Genetic Modified Soybean DNA Determination via Adsorption of Anthraquinone‐2‐sulphonic Acid in Reduced Graphene Oxide

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