Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots

Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots

In the current study, we investigated the chemical composition, in vitro antioxidant, antimicrobial and haemolytic activities of the crude (methanolic) and n-hexane, chloroform, and ethyl acetate extracts of roots of Crambe cordifolia Steven. The GC-MS analysis led to identification of erucic acid (...

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Main Authors: Rashid, Muhammad Abid, Akhtar, Muhammad Nadeem, Ashraf, Aisha, Nazir, Sadia, Ijaz, Anum, Noradila, Abdullah Omar, Noor, Sadia, Basit, Abdul, Tareen, Rasool Bakhsh
Format: Article
Language:English
Published: Romanian Society for Pharmaceutical Sciences 2018
Subjects:
QR Microbiology
Online Access:http://umpir.ump.edu.my/id/eprint/23930/
http://umpir.ump.edu.my/id/eprint/23930/
http://umpir.ump.edu.my/id/eprint/23930/1/Chemical%20composition%20and%20antioxidant.pdf
id ump-23930
recordtype eprints
spelling ump-239302019-01-23T08:02:18Z http://umpir.ump.edu.my/id/eprint/23930/ Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots Rashid, Muhammad Abid Akhtar, Muhammad Nadeem Ashraf, Aisha Nazir, Sadia Ijaz, Anum Noradila, Abdullah Omar Noor, Sadia Basit, Abdul Tareen, Rasool Bakhsh QR Microbiology In the current study, we investigated the chemical composition, in vitro antioxidant, antimicrobial and haemolytic activities of the crude (methanolic) and n-hexane, chloroform, and ethyl acetate extracts of roots of Crambe cordifolia Steven. The GC-MS analysis led to identification of erucic acid (20.8%), p-cymene (20.9%), palmitic acid (19.2%) and vitamin E (13.8%) as major components of methanol, n-hexane, chloroform and ethyl acetate extracts respectively. The results of spectrophotometric assays showed that the methanol extract had the highest total phenolic contents (210.3 ± 0.74 μg GAE/mg of dry plant material), total flavonoid contents (75.5 ± 0.32 μg QE/mg of dry plant material) and antioxidant potential. It was established by disc diffusion and minimum inhibitory concentration methods that, overall, the methanol extract exhibited the highest antimicrobial activity against most of the tested microbial strains (Escherichia coli, Bacillus subtilis, Pasteurella multocida, Staphylococcus aureus, Aspergillus niger and Fusarium solani) as compared with the nhexane, chloroform, and ethyl acetate extracts. The outcome of the haemolytic assay showed that the tested extract and different solvent fractions were safe to human erythrocyte membrane Romanian Society for Pharmaceutical Sciences 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/23930/1/Chemical%20composition%20and%20antioxidant.pdf Rashid, Muhammad Abid and Akhtar, Muhammad Nadeem and Ashraf, Aisha and Nazir, Sadia and Ijaz, Anum and Noradila, Abdullah Omar and Noor, Sadia and Basit, Abdul and Tareen, Rasool Bakhsh (2018) Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots. Farmacia, 66 (1). pp. 165-171. ISSN 2065-0019 http://www.revistafarmacia.ro/201801/art-21-Muhammad_Rashid_165-171.pdf
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QR Microbiology
spellingShingle QR Microbiology
Rashid, Muhammad Abid
Akhtar, Muhammad Nadeem
Ashraf, Aisha
Nazir, Sadia
Ijaz, Anum
Noradila, Abdullah Omar
Noor, Sadia
Basit, Abdul
Tareen, Rasool Bakhsh
Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots
description In the current study, we investigated the chemical composition, in vitro antioxidant, antimicrobial and haemolytic activities of the crude (methanolic) and n-hexane, chloroform, and ethyl acetate extracts of roots of Crambe cordifolia Steven. The GC-MS analysis led to identification of erucic acid (20.8%), p-cymene (20.9%), palmitic acid (19.2%) and vitamin E (13.8%) as major components of methanol, n-hexane, chloroform and ethyl acetate extracts respectively. The results of spectrophotometric assays showed that the methanol extract had the highest total phenolic contents (210.3 ± 0.74 μg GAE/mg of dry plant material), total flavonoid contents (75.5 ± 0.32 μg QE/mg of dry plant material) and antioxidant potential. It was established by disc diffusion and minimum inhibitory concentration methods that, overall, the methanol extract exhibited the highest antimicrobial activity against most of the tested microbial strains (Escherichia coli, Bacillus subtilis, Pasteurella multocida, Staphylococcus aureus, Aspergillus niger and Fusarium solani) as compared with the nhexane, chloroform, and ethyl acetate extracts. The outcome of the haemolytic assay showed that the tested extract and different solvent fractions were safe to human erythrocyte membrane
format Article
author Rashid, Muhammad Abid
Akhtar, Muhammad Nadeem
Ashraf, Aisha
Nazir, Sadia
Ijaz, Anum
Noradila, Abdullah Omar
Noor, Sadia
Basit, Abdul
Tareen, Rasool Bakhsh
author_facet Rashid, Muhammad Abid
Akhtar, Muhammad Nadeem
Ashraf, Aisha
Nazir, Sadia
Ijaz, Anum
Noradila, Abdullah Omar
Noor, Sadia
Basit, Abdul
Tareen, Rasool Bakhsh
author_sort Rashid, Muhammad Abid
title Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots
title_short Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots
title_full Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots
title_fullStr Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots
title_full_unstemmed Chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots
title_sort chemical composition and antioxidant, antimicrobial and haemolytic activities of crambe cordifolia roots
publisher Romanian Society for Pharmaceutical Sciences
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/23930/
http://umpir.ump.edu.my/id/eprint/23930/
http://umpir.ump.edu.my/id/eprint/23930/1/Chemical%20composition%20and%20antioxidant.pdf
first_indexed 2023-09-18T22:36:03Z
last_indexed 2023-09-18T22:36:03Z
_version_ 1777416602600341504

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