Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia

The multidrug resistant Escherichia coli strains causes multiple clinical infections and has become a rising problem globally. The metallo-β-lactamases encoding genes are very severe in gram-negative bacteria especially E. coli. This study was aimed to evaluate the prevalence of MBLs among the clini...

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Main Authors:	M. K. K., Fazlul, S., Deepthi, Y., Farzana, A., Najnin, M. A., Rashid, B., Munira, C., Srikumar, M. H. M., Nazmul
Format:	Article
Language:	English
Published:	2019
Subjects:	QR Microbiology RM Therapeutics. Pharmacology
Online Access:	http://umpir.ump.edu.my/id/eprint/24791/ http://umpir.ump.edu.my/id/eprint/24791/ http://umpir.ump.edu.my/id/eprint/24791/ http://umpir.ump.edu.my/id/eprint/24791/1/Detection%20of%20Metallo-B-Lactamases-Encoding1.pdf

id	ump-24791
recordtype	eprints
spelling	ump-247912019-04-22T08:35:13Z http://umpir.ump.edu.my/id/eprint/24791/ Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia M. K. K., Fazlul S., Deepthi Y., Farzana A., Najnin M. A., Rashid B., Munira C., Srikumar M. H. M., Nazmul QR Microbiology RM Therapeutics. Pharmacology The multidrug resistant Escherichia coli strains causes multiple clinical infections and has become a rising problem globally. The metallo-β-lactamases encoding genes are very severe in gram-negative bacteria especially E. coli. This study was aimed to evaluate the prevalence of MBLs among the clinical isolates of E. coli. A total of 65 E. coli isolates were collected from various clinical samples of Malaysian patients with bacterial infections. The conventional microbiological test was performed for isolation and identification of E. coli producing MBLs strains in this vicinity. Multidrug Resistance (MDR) of E. coli isolates were assessed using disk diffusion test. Phenotypic methods, as well as genotypic- PCR methods, were performed to detect the presence of metallo-β-lactamase resistance genes (blaIMP, blaVIM) in imipenem resistant strains. Out of 65 E. coli isolates, 42 isolates (57.3%) were MDR. The isolates from urine (19) produced significantly more MDR (10) isolates than other sources. Additionally, 19 (29.2%) imipenem- resistant E. coli isolates contained 10 MBLs gene, 7(36.8%) isolates contained blaIMP and 3(15.7%) isolates contained blaVIM genes. This study revealed the significant occurrence of MBL producing E. coli isolates in clinical specimens and its association with health risk factors indicating an alarming situation in Malaysia. It demands an appropriate concern to avoid failure of treatments and infection control management 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24791/1/Detection%20of%20Metallo-B-Lactamases-Encoding1.pdf M. K. K., Fazlul and S., Deepthi and Y., Farzana and A., Najnin and M. A., Rashid and B., Munira and C., Srikumar and M. H. M., Nazmul (2019) Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia. International Journal of Pharmaceutical Research, 11 (1). pp. 290-298. ISSN 0975-2366 http://www.ijpronline.com/ViewArticleDetail.aspx?ID=7406 https://doi.org/10.31838/ijpr/2019.11.01.031 0975-2366
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QR Microbiology RM Therapeutics. Pharmacology
spellingShingle	QR Microbiology RM Therapeutics. Pharmacology M. K. K., Fazlul S., Deepthi Y., Farzana A., Najnin M. A., Rashid B., Munira C., Srikumar M. H. M., Nazmul Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia
description	The multidrug resistant Escherichia coli strains causes multiple clinical infections and has become a rising problem globally. The metallo-β-lactamases encoding genes are very severe in gram-negative bacteria especially E. coli. This study was aimed to evaluate the prevalence of MBLs among the clinical isolates of E. coli. A total of 65 E. coli isolates were collected from various clinical samples of Malaysian patients with bacterial infections. The conventional microbiological test was performed for isolation and identification of E. coli producing MBLs strains in this vicinity. Multidrug Resistance (MDR) of E. coli isolates were assessed using disk diffusion test. Phenotypic methods, as well as genotypic- PCR methods, were performed to detect the presence of metallo-β-lactamase resistance genes (blaIMP, blaVIM) in imipenem resistant strains. Out of 65 E. coli isolates, 42 isolates (57.3%) were MDR. The isolates from urine (19) produced significantly more MDR (10) isolates than other sources. Additionally, 19 (29.2%) imipenem- resistant E. coli isolates contained 10 MBLs gene, 7(36.8%) isolates contained blaIMP and 3(15.7%) isolates contained blaVIM genes. This study revealed the significant occurrence of MBL producing E. coli isolates in clinical specimens and its association with health risk factors indicating an alarming situation in Malaysia. It demands an appropriate concern to avoid failure of treatments and infection control management
format	Article
author	M. K. K., Fazlul S., Deepthi Y., Farzana A., Najnin M. A., Rashid B., Munira C., Srikumar M. H. M., Nazmul
author_facet	M. K. K., Fazlul S., Deepthi Y., Farzana A., Najnin M. A., Rashid B., Munira C., Srikumar M. H. M., Nazmul
author_sort	M. K. K., Fazlul
title	Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia
title_short	Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia
title_full	Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia
title_fullStr	Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia
title_full_unstemmed	Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia
title_sort	detection of metallo-b-lactamases-encoding genes among clinical isolates of escherichia coli in a tertiary care hospital malaysia
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/24791/ http://umpir.ump.edu.my/id/eprint/24791/ http://umpir.ump.edu.my/id/eprint/24791/ http://umpir.ump.edu.my/id/eprint/24791/1/Detection%20of%20Metallo-B-Lactamases-Encoding1.pdf
first_indexed	2023-09-18T22:37:44Z
last_indexed	2023-09-18T22:37:44Z
_version_	1777416708147904512

Detection of Metallo-B-Lactamases-Encoding genes among clinical isolates of Escherichia Coli in a tertiary care hospital Malaysia

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