Isolation and identification of denitryifing from indegenous mircroorganism

The objective of this study was to isolate and identify novel potential denitrifying bacteria from two different Kuantan areas which are Jubli Perak Agricultural Park, Kuantan (3º50'49.6"N103º18'06.1"E) and Felda Lepar Hilir, Kuantan (3º40'41.4"N 103º03'24.7"E...

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Main Authors:	Maizatul Farhain, Ismail, Nurul 'Azyyati, Sabri, Saiful Nizam, Tajuddin, Lee, Chin Mei, Siti Hatijah, Mortan, Ab. Rahim, Mohd-Hairul
Format:	Article
Language:	English
Published:	The Official Publication of The Malaysian Society for Biochemistry & Molecular Biology (MSBMB) 2019
Subjects:	T Technology (General) TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/24871/ http://umpir.ump.edu.my/id/eprint/24871/ http://umpir.ump.edu.my/id/eprint/24871/1/Maizatul%20Farhain%20Ismail%20et%20al%20galley%20proof.pdf

id	ump-24871
recordtype	eprints
spelling	ump-248712019-05-08T07:06:30Z http://umpir.ump.edu.my/id/eprint/24871/ Isolation and identification of denitryifing from indegenous mircroorganism Maizatul Farhain, Ismail Nurul 'Azyyati, Sabri Saiful Nizam, Tajuddin Lee, Chin Mei Siti Hatijah, Mortan Ab. Rahim, Mohd-Hairul T Technology (General) TP Chemical technology The objective of this study was to isolate and identify novel potential denitrifying bacteria from two different Kuantan areas which are Jubli Perak Agricultural Park, Kuantan (3º50'49.6"N103º18'06.1"E) and Felda Lepar Hilir, Kuantan (3º40'41.4"N 103º03'24.7"E). Indigenous Microorganisms (IMOs) available in the locations were cultivated by fermentation of steamed rice in the location together with brown sugar. Serial dilutions of the fermented medium were spread on Jensen’s agar and incubated at 30 ºC for eight days. A total of six colonies were subjected to various biochemical analysis including Gram staining, catalase, methyl red, carbohydrate fermentation and nitrate reduction tests. All bacterial isolates were subjected to genomic DNA extraction and PCR amplification of 16S rRNA genes using 27F and 1942R primers. All the amplified product of 16S rRNA genes from the bacterial isolates were purified and sent for sequencing. BLASTn and phylogenetic analysis based on 16S rRNA gene sequences shown all the isolates belong to Bacillus spp. and clustered into two main clusters. The Official Publication of The Malaysian Society for Biochemistry & Molecular Biology (MSBMB) 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24871/1/Maizatul%20Farhain%20Ismail%20et%20al%20galley%20proof.pdf Maizatul Farhain, Ismail and Nurul 'Azyyati, Sabri and Saiful Nizam, Tajuddin and Lee, Chin Mei and Siti Hatijah, Mortan and Ab. Rahim, Mohd-Hairul (2019) Isolation and identification of denitryifing from indegenous mircroorganism. Malaysian Journal Of Biochemistry & Molecular Biology (1). pp. 17-21. ISSN ISSN: 1511-2616, E-ISSN 2600-9005, http://mjbmb.org
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	T Technology (General) TP Chemical technology
spellingShingle	T Technology (General) TP Chemical technology Maizatul Farhain, Ismail Nurul 'Azyyati, Sabri Saiful Nizam, Tajuddin Lee, Chin Mei Siti Hatijah, Mortan Ab. Rahim, Mohd-Hairul Isolation and identification of denitryifing from indegenous mircroorganism
description	The objective of this study was to isolate and identify novel potential denitrifying bacteria from two different Kuantan areas which are Jubli Perak Agricultural Park, Kuantan (3º50'49.6"N103º18'06.1"E) and Felda Lepar Hilir, Kuantan (3º40'41.4"N 103º03'24.7"E). Indigenous Microorganisms (IMOs) available in the locations were cultivated by fermentation of steamed rice in the location together with brown sugar. Serial dilutions of the fermented medium were spread on Jensen’s agar and incubated at 30 ºC for eight days. A total of six colonies were subjected to various biochemical analysis including Gram staining, catalase, methyl red, carbohydrate fermentation and nitrate reduction tests. All bacterial isolates were subjected to genomic DNA extraction and PCR amplification of 16S rRNA genes using 27F and 1942R primers. All the amplified product of 16S rRNA genes from the bacterial isolates were purified and sent for sequencing. BLASTn and phylogenetic analysis based on 16S rRNA gene sequences shown all the isolates belong to Bacillus spp. and clustered into two main clusters.
format	Article
author	Maizatul Farhain, Ismail Nurul 'Azyyati, Sabri Saiful Nizam, Tajuddin Lee, Chin Mei Siti Hatijah, Mortan Ab. Rahim, Mohd-Hairul
author_facet	Maizatul Farhain, Ismail Nurul 'Azyyati, Sabri Saiful Nizam, Tajuddin Lee, Chin Mei Siti Hatijah, Mortan Ab. Rahim, Mohd-Hairul
author_sort	Maizatul Farhain, Ismail
title	Isolation and identification of denitryifing from indegenous mircroorganism
title_short	Isolation and identification of denitryifing from indegenous mircroorganism
title_full	Isolation and identification of denitryifing from indegenous mircroorganism
title_fullStr	Isolation and identification of denitryifing from indegenous mircroorganism
title_full_unstemmed	Isolation and identification of denitryifing from indegenous mircroorganism
title_sort	isolation and identification of denitryifing from indegenous mircroorganism
publisher	The Official Publication of The Malaysian Society for Biochemistry & Molecular Biology (MSBMB)
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/24871/ http://umpir.ump.edu.my/id/eprint/24871/ http://umpir.ump.edu.my/id/eprint/24871/1/Maizatul%20Farhain%20Ismail%20et%20al%20galley%20proof.pdf
first_indexed	2023-09-18T22:37:53Z
last_indexed	2023-09-18T22:37:53Z
_version_	1777416717938458624

Isolation and identification of denitryifing from indegenous mircroorganism

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