Whey protein fractionation based on sp-sepharose cation exchange chromatography

Whey protein fractionation based on sp-sepharose cation exchange chromatography

Bovine whey protein consists of different type of proteins such as βlactoglobulin (β-lag), α-lactalbumin (α-lac), immunoglobulin, bovine serum albumin (BSA), lactoferrin, lactoperoxidase and glycomacropeptide. Single protein is more valuable compare to the mixture of proteins and it can be used for...

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Main Author: Hazman, Abd Mulok
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/2499/
http://umpir.ump.edu.my/id/eprint/2499/1/CD5640_HAZMAN_BIN_ABD_MULOK.pdf
id ump-2499
recordtype eprints
spelling ump-24992015-03-03T07:56:58Z http://umpir.ump.edu.my/id/eprint/2499/ Whey protein fractionation based on sp-sepharose cation exchange chromatography Hazman, Abd Mulok TP Chemical technology Bovine whey protein consists of different type of proteins such as βlactoglobulin (β-lag), α-lactalbumin (α-lac), immunoglobulin, bovine serum albumin (BSA), lactoferrin, lactoperoxidase and glycomacropeptide. Single protein is more valuable compare to the mixture of proteins and it can be used for specific application. The main objective of this study is to fractionate whey protein components into several groups or single protein fraction using SP-sepharose TM Fast Flow (Amersham Biosciences) cation exchange chromatography column. The chromatographic process was run at different pH range from pH 4 to pH 8 using 24 ml column. All major whey proteins were recovered in the elution fraction at pH 4 and pH 4.5. No protein was bound at pH 7 and pH 8. The best whey fractionation from 2 ml whey feed was achieved at pH 4 with the yield for β-lag, BSA and α-lac are 83.33%, 66.44% and 10.51% respectively. Different protein fraction recovered during fractionation process at different pH using cation exchange chromatography process, can be used as a guideline to isolate particular protein of interest from bovine whey 2010-11 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2499/1/CD5640_HAZMAN_BIN_ABD_MULOK.pdf Hazman, Abd Mulok (2010) Whey protein fractionation based on sp-sepharose cation exchange chromatography. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang .
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Hazman, Abd Mulok
Whey protein fractionation based on sp-sepharose cation exchange chromatography
description Bovine whey protein consists of different type of proteins such as βlactoglobulin (β-lag), α-lactalbumin (α-lac), immunoglobulin, bovine serum albumin (BSA), lactoferrin, lactoperoxidase and glycomacropeptide. Single protein is more valuable compare to the mixture of proteins and it can be used for specific application. The main objective of this study is to fractionate whey protein components into several groups or single protein fraction using SP-sepharose TM Fast Flow (Amersham Biosciences) cation exchange chromatography column. The chromatographic process was run at different pH range from pH 4 to pH 8 using 24 ml column. All major whey proteins were recovered in the elution fraction at pH 4 and pH 4.5. No protein was bound at pH 7 and pH 8. The best whey fractionation from 2 ml whey feed was achieved at pH 4 with the yield for β-lag, BSA and α-lac are 83.33%, 66.44% and 10.51% respectively. Different protein fraction recovered during fractionation process at different pH using cation exchange chromatography process, can be used as a guideline to isolate particular protein of interest from bovine whey
format Undergraduates Project Papers
author Hazman, Abd Mulok
author_facet Hazman, Abd Mulok
author_sort Hazman, Abd Mulok
title Whey protein fractionation based on sp-sepharose cation exchange chromatography
title_short Whey protein fractionation based on sp-sepharose cation exchange chromatography
title_full Whey protein fractionation based on sp-sepharose cation exchange chromatography
title_fullStr Whey protein fractionation based on sp-sepharose cation exchange chromatography
title_full_unstemmed Whey protein fractionation based on sp-sepharose cation exchange chromatography
title_sort whey protein fractionation based on sp-sepharose cation exchange chromatography
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/2499/
http://umpir.ump.edu.my/id/eprint/2499/1/CD5640_HAZMAN_BIN_ABD_MULOK.pdf
first_indexed 2023-09-18T21:56:22Z
last_indexed 2023-09-18T21:56:22Z
_version_ 1777414105560252416

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