Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors

The medium of mouse-mouse hybridoma, 192 producing IgG against 17-OHP was supplemented with seven different inducing agents at different range namely lysozyme, lipopolysaccharides (LPS), potassium phosphate, aldolase, essential amino acid, non-essential amino acid and sodium butyrate. This study aim...

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Main Author:	Muhd Abd Mursyid, W. Mohd. Saberi
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/2509/ http://umpir.ump.edu.my/id/eprint/2509/ http://umpir.ump.edu.my/id/eprint/2509/1/CD5672_MUHD_ABD_MURSYID_B_W_MOHD.SABERI.pdf

id	ump-2509
recordtype	eprints
spelling	ump-25092015-03-03T07:57:01Z http://umpir.ump.edu.my/id/eprint/2509/ Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors Muhd Abd Mursyid, W. Mohd. Saberi TP Chemical technology The medium of mouse-mouse hybridoma, 192 producing IgG against 17-OHP was supplemented with seven different inducing agents at different range namely lysozyme, lipopolysaccharides (LPS), potassium phosphate, aldolase, essential amino acid, non-essential amino acid and sodium butyrate. This study aims to compare the statistical design of experiments (DOE) and one-factor-at-a-time (OFAT) method in screening immunoglobulin production stimulating factors. The culture medium supplemented with the inducer agents were screened using OFAT method and Plakett- Burman design method to determine inducer agent that gave positive effect. The effect of inducer concentration towards the antibody production was studied using enzyme-linked immunosorbent assay (ELISA) technique. The optimal concentrations for all the inducing agent were failed to be found in OFAT method. The standard culture medium without addition of inducer yield 0.234μg/ml of MAb. The culture supplemented with the inducer agent that yields MAb close to the standard was non-essential amino acid. Plakett-Burman design gave 12 runs with only the high level (+) and the low level (-) of the inducer were used. Potassium phosphate, LPS and sodium butyrate was found to be the most influential variable using Plakett-Burman design. Plakett-Burman design however indicates that all the point was insignificant and the interaction between inducer was not described. Plakett-Burman under DOE method gave the best time-wise compared to OFAT method in screening monoclonal antibody stimulating factors. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2509/1/CD5672_MUHD_ABD_MURSYID_B_W_MOHD.SABERI.pdf Muhd Abd Mursyid, W. Mohd. Saberi (2010) Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang . http://iportal.ump.edu.my/lib/item?id=chamo:57496&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Muhd Abd Mursyid, W. Mohd. Saberi Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors
description	The medium of mouse-mouse hybridoma, 192 producing IgG against 17-OHP was supplemented with seven different inducing agents at different range namely lysozyme, lipopolysaccharides (LPS), potassium phosphate, aldolase, essential amino acid, non-essential amino acid and sodium butyrate. This study aims to compare the statistical design of experiments (DOE) and one-factor-at-a-time (OFAT) method in screening immunoglobulin production stimulating factors. The culture medium supplemented with the inducer agents were screened using OFAT method and Plakett- Burman design method to determine inducer agent that gave positive effect. The effect of inducer concentration towards the antibody production was studied using enzyme-linked immunosorbent assay (ELISA) technique. The optimal concentrations for all the inducing agent were failed to be found in OFAT method. The standard culture medium without addition of inducer yield 0.234μg/ml of MAb. The culture supplemented with the inducer agent that yields MAb close to the standard was non-essential amino acid. Plakett-Burman design gave 12 runs with only the high level (+) and the low level (-) of the inducer were used. Potassium phosphate, LPS and sodium butyrate was found to be the most influential variable using Plakett-Burman design. Plakett-Burman design however indicates that all the point was insignificant and the interaction between inducer was not described. Plakett-Burman under DOE method gave the best time-wise compared to OFAT method in screening monoclonal antibody stimulating factors.
format	Undergraduates Project Papers
author	Muhd Abd Mursyid, W. Mohd. Saberi
author_facet	Muhd Abd Mursyid, W. Mohd. Saberi
author_sort	Muhd Abd Mursyid, W. Mohd. Saberi
title	Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors
title_short	Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors
title_full	Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors
title_fullStr	Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors
title_full_unstemmed	Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors
title_sort	comparison between mehod of one-factor-at-a-time (ofat) & design of experiment (doe) in screening of immunoglobulin production stimulatesing factors
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/2509/ http://umpir.ump.edu.my/id/eprint/2509/ http://umpir.ump.edu.my/id/eprint/2509/1/CD5672_MUHD_ABD_MURSYID_B_W_MOHD.SABERI.pdf
first_indexed	2023-09-18T21:56:23Z
last_indexed	2023-09-18T21:56:23Z
_version_	1777414106870972416

Comparison between mehod of one-factor-at-a-time (OFAT) & design of experiment (DOE) in screening of immunoglobulin production stimulatesing factors

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