An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres

An electrochemical microbiosensor for DNA has been fabricated based on new acrylic microspheres modified with reactive N-acryloxysuccinimide (NAS) functional groups. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesized in an emulsion form with a simple one-step photo...

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Main Authors:	Ulianas, Alizar, Heng, Lee Yook, Sharina, Abu Hanifah, Tan, Ling Ling
Format:	Article
Language:	English
Published:	MDPI 2012
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/25315/ http://umpir.ump.edu.my/id/eprint/25315/ http://umpir.ump.edu.my/id/eprint/25315/ http://umpir.ump.edu.my/id/eprint/25315/1/An%20electrochemical%20DNA%20microbiosensor%20based%20on%20succinimide-modified%20acrylic%20microspheres.pdf

id	ump-25315
recordtype	eprints
spelling	ump-253152019-12-09T04:40:09Z http://umpir.ump.edu.my/id/eprint/25315/ An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres Ulianas, Alizar Heng, Lee Yook Sharina, Abu Hanifah Tan, Ling Ling Q Science (General) T Technology (General) An electrochemical microbiosensor for DNA has been fabricated based on new acrylic microspheres modified with reactive N-acryloxysuccinimide (NAS) functional groups. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesized in an emulsion form with a simple one-step photopolymerization technique. Aminated DNA probe was attached to the succinimde functional group of the acrylic microspheres via covalent bonding. The hybridization of the immobilized DNA probe with the complementary DNA was studied by differential pulse voltametry using anthraquninone-2-sulfonic acid monohydrate sodium salt (AQMS) as the electroactive hybridization label. The influences of many factors such as duration of DNA probe immobilization and hybridization, pH, type of ions, buffer concentrations, ionic strength, operational temperature and non-complementary DNA on the biosensor performance were evaluated. Under optimized conditions, the DNA microbiosensor demonstrated a linear response range to target DNA over a wide concentration range of 1.0 × 10−16 and 1.0 ´ 10−8 M with a lower limit of detection (LOD) of 9.46 ´ 10−17 M (R2 = 0.97). This DNA microbiosensor showed good reproducibility with 2.84% RSD (relative standard deviation) (n = 3). Application of the NAS-modified acrylic microspheres in the construction of DNA microbiosensor had improved the overall analytical performance of the resultant DNA microbiosensor when compared with other reported DNA biosensors using other nano-materials for membranes and microspheres as DNA immobilization matrices. MDPI 2012 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25315/1/An%20electrochemical%20DNA%20microbiosensor%20based%20on%20succinimide-modified%20acrylic%20microspheres.pdf Ulianas, Alizar and Heng, Lee Yook and Sharina, Abu Hanifah and Tan, Ling Ling (2012) An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres. Sensors, 12 (5). pp. 5445-5460. ISSN 1424-8220 https://doi.org/10.3390/s120505445 https://doi.org/10.3390/s120505445
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) Ulianas, Alizar Heng, Lee Yook Sharina, Abu Hanifah Tan, Ling Ling An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres
description	An electrochemical microbiosensor for DNA has been fabricated based on new acrylic microspheres modified with reactive N-acryloxysuccinimide (NAS) functional groups. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesized in an emulsion form with a simple one-step photopolymerization technique. Aminated DNA probe was attached to the succinimde functional group of the acrylic microspheres via covalent bonding. The hybridization of the immobilized DNA probe with the complementary DNA was studied by differential pulse voltametry using anthraquninone-2-sulfonic acid monohydrate sodium salt (AQMS) as the electroactive hybridization label. The influences of many factors such as duration of DNA probe immobilization and hybridization, pH, type of ions, buffer concentrations, ionic strength, operational temperature and non-complementary DNA on the biosensor performance were evaluated. Under optimized conditions, the DNA microbiosensor demonstrated a linear response range to target DNA over a wide concentration range of 1.0 × 10−16 and 1.0 ´ 10−8 M with a lower limit of detection (LOD) of 9.46 ´ 10−17 M (R2 = 0.97). This DNA microbiosensor showed good reproducibility with 2.84% RSD (relative standard deviation) (n = 3). Application of the NAS-modified acrylic microspheres in the construction of DNA microbiosensor had improved the overall analytical performance of the resultant DNA microbiosensor when compared with other reported DNA biosensors using other nano-materials for membranes and microspheres as DNA immobilization matrices.
format	Article
author	Ulianas, Alizar Heng, Lee Yook Sharina, Abu Hanifah Tan, Ling Ling
author_facet	Ulianas, Alizar Heng, Lee Yook Sharina, Abu Hanifah Tan, Ling Ling
author_sort	Ulianas, Alizar
title	An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres
title_short	An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres
title_full	An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres
title_fullStr	An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres
title_full_unstemmed	An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres
title_sort	electrochemical dna microbiosensor based on succinimide-modified acrylic microspheres
publisher	MDPI
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/25315/ http://umpir.ump.edu.my/id/eprint/25315/ http://umpir.ump.edu.my/id/eprint/25315/ http://umpir.ump.edu.my/id/eprint/25315/1/An%20electrochemical%20DNA%20microbiosensor%20based%20on%20succinimide-modified%20acrylic%20microspheres.pdf
first_indexed	2023-09-18T22:38:49Z
last_indexed	2023-09-18T22:38:49Z
_version_	1777416776271790080

An electrochemical DNA microbiosensor based on succinimide-modified acrylic microspheres

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