Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles

Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles

This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH...

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Main Authors: Sharifah Fathiyah, Sy Mohamad, Farhan, Mohd Said, Mimi Sakinah, A. M., Shahril, Mohamad
Format: Article
Language:English
Published: Department of Chemistry, Universitas Gadjah Mada Yogyakarta 2019
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/25736/
http://umpir.ump.edu.my/id/eprint/25736/
http://umpir.ump.edu.my/id/eprint/25736/
http://umpir.ump.edu.my/id/eprint/25736/1/Effect%20of%20key%20parameters%20on%20jacalin%20extraction.pdf
id ump-25736
recordtype eprints
spelling ump-257362019-11-22T04:09:05Z http://umpir.ump.edu.my/id/eprint/25736/ Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles Sharifah Fathiyah, Sy Mohamad Farhan, Mohd Said Mimi Sakinah, A. M. Shahril, Mohamad TP Chemical technology This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH 4 – 10), AOT concentration (5 – 150 mM), contact time (5 – 30 min) and phase volume ratio (0.5-5) on the transfer efficiency of jacalin was evaluated by changing one factor at a time (OFAT) while keeping the other parameters constant. A maximum of 83% of protein transfer was achieved after equal volume of organic and aqueous phase was stirred for 20 min using 20 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture. Department of Chemistry, Universitas Gadjah Mada Yogyakarta 2019-08 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/25736/1/Effect%20of%20key%20parameters%20on%20jacalin%20extraction.pdf Sharifah Fathiyah, Sy Mohamad and Farhan, Mohd Said and Mimi Sakinah, A. M. and Shahril, Mohamad (2019) Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles. Indonesian Journal of Chemistry, 19 (3). pp. 737-746. ISSN 1411-9420 (Print); 2460-1578 (Online) https://doi.org/10.22146/ijc.39705 https://doi.org/10.22146/ijc.39705
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Sharifah Fathiyah, Sy Mohamad
Farhan, Mohd Said
Mimi Sakinah, A. M.
Shahril, Mohamad
Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles
description This study demonstrates the extraction of jacalin from crude extract of jackfruit seeds using anionic surfactant based reverse micellar system, sodium bis(2-ethylhexyl) sulfosuccinate (AOT) in isooctane. Effects of various parameters, such as, NaCl concentration (0.05 – 1 M), pH of aqueous phase (pH 4 – 10), AOT concentration (5 – 150 mM), contact time (5 – 30 min) and phase volume ratio (0.5-5) on the transfer efficiency of jacalin was evaluated by changing one factor at a time (OFAT) while keeping the other parameters constant. A maximum of 83% of protein transfer was achieved after equal volume of organic and aqueous phase was stirred for 20 min using 20 mM AOT at pH 5 aqueous phase containing 0.1 M NaCl. The findings demonstrated AOT reverse micellar system as a promising and effective method to extract and purify jacalin from crude protein mixture.
format Article
author Sharifah Fathiyah, Sy Mohamad
Farhan, Mohd Said
Mimi Sakinah, A. M.
Shahril, Mohamad
author_facet Sharifah Fathiyah, Sy Mohamad
Farhan, Mohd Said
Mimi Sakinah, A. M.
Shahril, Mohamad
author_sort Sharifah Fathiyah, Sy Mohamad
title Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles
title_short Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles
title_full Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles
title_fullStr Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles
title_full_unstemmed Effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles
title_sort effect of key parameters on jacalin extraction from aqueous phase into anionic reverse micelles
publisher Department of Chemistry, Universitas Gadjah Mada Yogyakarta
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/25736/
http://umpir.ump.edu.my/id/eprint/25736/
http://umpir.ump.edu.my/id/eprint/25736/
http://umpir.ump.edu.my/id/eprint/25736/1/Effect%20of%20key%20parameters%20on%20jacalin%20extraction.pdf
first_indexed 2023-09-18T22:39:42Z
last_indexed 2023-09-18T22:39:42Z
_version_ 1777416832192348160

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