Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3)

Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the grow...

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Main Authors:	Rafidah, Mohamed Ali Hanapiah, Rozaimi, Abu Samah, Nurul Atikah, Mohd Rodzri, Mior Ahmad Khushairi, Mohd Zahari, Wan Salwanis, Wan Md. Zain
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/25948/ http://umpir.ump.edu.my/id/eprint/25948/1/Effects%20of%20culture%20conditions%20on%20growth%20and%20production1.pdf

id	ump-25948
recordtype	eprints
spelling	ump-259482019-10-16T03:39:00Z http://umpir.ump.edu.my/id/eprint/25948/ Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3) Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Nurul Atikah, Mohd Rodzri Mior Ahmad Khushairi, Mohd Zahari Wan Salwanis, Wan Md. Zain TP Chemical technology Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the growth of the recombinant E. coli BL21 (DE3) and xylonic acid production were investigated. The fermentation was carried out in shake flasks for 24 h at 37 °C and 200 rpm. Hydroxamate analysis was performed to determine the concentration of xylonic acid. The recombinant E. coli BL21 (DE3) produced up to 8.69 g/L xylonic acid with yield of 0.86 g/g from 10 g/L xylose in SOB medium with 20 g/L tryptone and 5 g/L yeast extract. 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25948/1/Effects%20of%20culture%20conditions%20on%20growth%20and%20production1.pdf Rafidah, Mohamed Ali Hanapiah and Rozaimi, Abu Samah and Nurul Atikah, Mohd Rodzri and Mior Ahmad Khushairi, Mohd Zahari and Wan Salwanis, Wan Md. Zain (2018) Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3). In: International Conference on Chemical Sciences and Engineering (ICCSE 2018), 7-8 November 2018 , Melaka, Malaysia. . (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Nurul Atikah, Mohd Rodzri Mior Ahmad Khushairi, Mohd Zahari Wan Salwanis, Wan Md. Zain Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3)
description	Xylonic acid is one of the top 30 highly valuable chemicals that can be obtained by microbial conversion. One of the recently used microorganisms to produce xylonic acid from xylose is the recombinant Escherichia coli. In this study, the effect of medium and nitrogen source concentration on the growth of the recombinant E. coli BL21 (DE3) and xylonic acid production were investigated. The fermentation was carried out in shake flasks for 24 h at 37 °C and 200 rpm. Hydroxamate analysis was performed to determine the concentration of xylonic acid. The recombinant E. coli BL21 (DE3) produced up to 8.69 g/L xylonic acid with yield of 0.86 g/g from 10 g/L xylose in SOB medium with 20 g/L tryptone and 5 g/L yeast extract.
format	Conference or Workshop Item
author	Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Nurul Atikah, Mohd Rodzri Mior Ahmad Khushairi, Mohd Zahari Wan Salwanis, Wan Md. Zain
author_facet	Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Nurul Atikah, Mohd Rodzri Mior Ahmad Khushairi, Mohd Zahari Wan Salwanis, Wan Md. Zain
author_sort	Rafidah, Mohamed Ali Hanapiah
title	Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3)
title_short	Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3)
title_full	Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3)
title_fullStr	Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3)
title_full_unstemmed	Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3)
title_sort	effects of culture conditions on growth and production of xylonic acid using recombinant e. coli bl21 (de3)
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/25948/ http://umpir.ump.edu.my/id/eprint/25948/1/Effects%20of%20culture%20conditions%20on%20growth%20and%20production1.pdf
first_indexed	2023-09-18T22:40:07Z
last_indexed	2023-09-18T22:40:07Z
_version_	1777416858387873792

Effects of culture conditions on growth and production of xylonic acid using recombinant E. coli BL21 (DE3)

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