Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)

ᴅ-xylonic acid is a five-carbon organic acid that has gain increasing interest due to its broadly flexible application in fields such as foods, pharmaceutical and agriculture. Microbial production of ᴅ-xylonic acid can be done by using many species of Pseudomonas, Acetobacter, Gluconobacter and the...

Full description

Bibliographic Details
Main Authors:	Nurul Atikah, Mohd Rodzri, Wan Salwanis, Wan Md. Zain, Rafidah, Mohamed Ali Hanapiah, Rozaimi, Abu Samah
Format:	Conference or Workshop Item
Language:	English
Published:	2018
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/25949/ http://umpir.ump.edu.my/id/eprint/25949/1/effect%20of%20pyhsical%20parameters.pdf

id	ump-25949
recordtype	eprints
spelling	ump-259492019-10-01T04:27:28Z http://umpir.ump.edu.my/id/eprint/25949/ Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3) Nurul Atikah, Mohd Rodzri Wan Salwanis, Wan Md. Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah TP Chemical technology ᴅ-xylonic acid is a five-carbon organic acid that has gain increasing interest due to its broadly flexible application in fields such as foods, pharmaceutical and agriculture. Microbial production of ᴅ-xylonic acid can be done by using many species of Pseudomonas, Acetobacter, Gluconobacter and the latest finding was by engineered microorganism. In this study, recombinant E. coli BL21 (DE3) is used to produce ᴅ-xylonic acid from ᴅ-xylose and the effect of temperature, initial pH of medium and agitation rate on ᴅ-xylonic acid production is determined. Fermentation was carried out using super optimal broth medium in shake flasks for 24 hours. Results indicate that ᴅ-xylonic acid concentration was as high as 9.95 g L-1 with yield of 0.91 g/g ᴅ-xylose from 10 g/L when governed under temperature of 37°C, initial pH of medium pH 7 and agitation rate 200 rpm. 2018 Conference or Workshop Item NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25949/1/effect%20of%20pyhsical%20parameters.pdf Nurul Atikah, Mohd Rodzri and Wan Salwanis, Wan Md. Zain and Rafidah, Mohamed Ali Hanapiah and Rozaimi, Abu Samah (2018) Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3). In: International Conference on Chemical Sciences and Engineering (ICCSE 2018), 7-8 November 2018 , Melaka, Malaysia. . (Submitted)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nurul Atikah, Mohd Rodzri Wan Salwanis, Wan Md. Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)
description	ᴅ-xylonic acid is a five-carbon organic acid that has gain increasing interest due to its broadly flexible application in fields such as foods, pharmaceutical and agriculture. Microbial production of ᴅ-xylonic acid can be done by using many species of Pseudomonas, Acetobacter, Gluconobacter and the latest finding was by engineered microorganism. In this study, recombinant E. coli BL21 (DE3) is used to produce ᴅ-xylonic acid from ᴅ-xylose and the effect of temperature, initial pH of medium and agitation rate on ᴅ-xylonic acid production is determined. Fermentation was carried out using super optimal broth medium in shake flasks for 24 hours. Results indicate that ᴅ-xylonic acid concentration was as high as 9.95 g L-1 with yield of 0.91 g/g ᴅ-xylose from 10 g/L when governed under temperature of 37°C, initial pH of medium pH 7 and agitation rate 200 rpm.
format	Conference or Workshop Item
author	Nurul Atikah, Mohd Rodzri Wan Salwanis, Wan Md. Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah
author_facet	Nurul Atikah, Mohd Rodzri Wan Salwanis, Wan Md. Zain Rafidah, Mohamed Ali Hanapiah Rozaimi, Abu Samah
author_sort	Nurul Atikah, Mohd Rodzri
title	Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)
title_short	Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)
title_full	Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)
title_fullStr	Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)
title_full_unstemmed	Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)
title_sort	effect of physical parameters on ᴅ-xylonic acid production using recombinant e. coli bl21 (de3)
publishDate	2018
url	http://umpir.ump.edu.my/id/eprint/25949/ http://umpir.ump.edu.my/id/eprint/25949/1/effect%20of%20pyhsical%20parameters.pdf
first_indexed	2023-09-18T22:40:07Z
last_indexed	2023-09-18T22:40:07Z
_version_	1777416858540965888

Effect of physical parameters on ᴅ-xylonic acid production using recombinant E. coli BL21 (DE3)

Similar Items