Oleate induces apoptosis in 3T3-L1 adipocytes

Oleic acid (C18:1) play an important role in proliferation of fat cells. In this study, the effect of oleate on cells viability in 3T3-L1 cells (fat cells) was investigated. The 3T3-L1 cells were treated with various concentrations of oleate in the presence of 23 mM glucose. Oleate was added to adip...

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Main Authors:	Rohana, Abu, Fadzilah Adibah, A. M., Muhammad Roji, M. S.
Format:	Article
Language:	English
Published:	World Academy of Science, Engineering and Technology 2011
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/26173/ http://umpir.ump.edu.my/id/eprint/26173/ http://umpir.ump.edu.my/id/eprint/26173/7/Oleate%20Induces%20Apoptosis%20in%203%20T%203L%201%20Adipocytes.pdf

id	ump-26173
recordtype	eprints
spelling	ump-261732020-02-25T07:38:55Z http://umpir.ump.edu.my/id/eprint/26173/ Oleate induces apoptosis in 3T3-L1 adipocytes Rohana, Abu Fadzilah Adibah, A. M. Muhammad Roji, M. S. TP Chemical technology Oleic acid (C18:1) play an important role in proliferation of fat cells. In this study, the effect of oleate on cells viability in 3T3-L1 cells (fat cells) was investigated. The 3T3-L1 cells were treated with various concentrations of oleate in the presence of 23 mM glucose. Oleate was added to adipogenic media (day 0) to investigate the influence of oleate on proliferation of postconfluent preadipocytes after 24 h induction. 0.1 mM oleate promoted cell division by increasing 33.9% number of cells from basal control in postconfluent preadipocytes. However, there were no significantly different in cells viability with control cells when oleate concentrations were increased up to 0.5 mM. When added to differentiated adipocytes (day 12) for 48 h, the number of cells decreased as oleate concentrations increased. 92.7% of cells lost demonstrated apoptosis and necrosis after 48 h with 0.5 mM oleate. The fluorochrome staining was examined under fluorescence microscopy using acridine orange and ethidium bromide double staining. Furthermore, the presence of high lactate (60.6% increased from basal control) released into plasma has shown the direct cytotoxicity of 0.5 mM oleate on adipocytes. Keywords—adipocytes, apoptosis, oleate, postconfluent preadipocytes. World Academy of Science, Engineering and Technology 2011 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/26173/7/Oleate%20Induces%20Apoptosis%20in%203%20T%203L%201%20Adipocytes.pdf Rohana, Abu and Fadzilah Adibah, A. M. and Muhammad Roji, M. S. (2011) Oleate induces apoptosis in 3T3-L1 adipocytes. International Journal of Biotechnology and Bioengineering, 5 (9). pp. 504-507. ISSN 2475-3432 https://publications.waset.org/search?q=Oleate+induces+apoptosis+in+3T3-L1+adipocytes
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rohana, Abu Fadzilah Adibah, A. M. Muhammad Roji, M. S. Oleate induces apoptosis in 3T3-L1 adipocytes
description	Oleic acid (C18:1) play an important role in proliferation of fat cells. In this study, the effect of oleate on cells viability in 3T3-L1 cells (fat cells) was investigated. The 3T3-L1 cells were treated with various concentrations of oleate in the presence of 23 mM glucose. Oleate was added to adipogenic media (day 0) to investigate the influence of oleate on proliferation of postconfluent preadipocytes after 24 h induction. 0.1 mM oleate promoted cell division by increasing 33.9% number of cells from basal control in postconfluent preadipocytes. However, there were no significantly different in cells viability with control cells when oleate concentrations were increased up to 0.5 mM. When added to differentiated adipocytes (day 12) for 48 h, the number of cells decreased as oleate concentrations increased. 92.7% of cells lost demonstrated apoptosis and necrosis after 48 h with 0.5 mM oleate. The fluorochrome staining was examined under fluorescence microscopy using acridine orange and ethidium bromide double staining. Furthermore, the presence of high lactate (60.6% increased from basal control) released into plasma has shown the direct cytotoxicity of 0.5 mM oleate on adipocytes. Keywords—adipocytes, apoptosis, oleate, postconfluent preadipocytes.
format	Article
author	Rohana, Abu Fadzilah Adibah, A. M. Muhammad Roji, M. S.
author_facet	Rohana, Abu Fadzilah Adibah, A. M. Muhammad Roji, M. S.
author_sort	Rohana, Abu
title	Oleate induces apoptosis in 3T3-L1 adipocytes
title_short	Oleate induces apoptosis in 3T3-L1 adipocytes
title_full	Oleate induces apoptosis in 3T3-L1 adipocytes
title_fullStr	Oleate induces apoptosis in 3T3-L1 adipocytes
title_full_unstemmed	Oleate induces apoptosis in 3T3-L1 adipocytes
title_sort	oleate induces apoptosis in 3t3-l1 adipocytes
publisher	World Academy of Science, Engineering and Technology
publishDate	2011
url	http://umpir.ump.edu.my/id/eprint/26173/ http://umpir.ump.edu.my/id/eprint/26173/ http://umpir.ump.edu.my/id/eprint/26173/7/Oleate%20Induces%20Apoptosis%20in%203%20T%203L%201%20Adipocytes.pdf
first_indexed	2023-09-18T22:40:35Z
last_indexed	2023-09-18T22:40:35Z
_version_	1777416888356175872

Oleate induces apoptosis in 3T3-L1 adipocytes

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