Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP

Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP

A Biochip is a platform of miniaturized microarrays arranged on a solid substrate that permits many tests to be performed at the same time in order to achieve higher throughput and speed. Single-cell identification, imaging and analysis in situ using Atomic Force Microscopy (AFM) faced a lot of diff...

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Main Authors: Fahmi, Samsuri, Alkaisi, M. M., Evans, J. J.
Format: Conference or Workshop Item
Language:English
Published: IEEE 2011
Subjects:
TK Electrical engineering. Electronics Nuclear engineering
Online Access:http://umpir.ump.edu.my/id/eprint/26228/
http://umpir.ump.edu.my/id/eprint/26228/
http://umpir.ump.edu.my/id/eprint/26228/1/Biochip%20development%20using%20Nanoimprint%20Lithography%20%28NIL%29%20and%20metallic%20.pdf
id ump-26228
recordtype eprints
spelling ump-262282020-02-12T03:01:02Z http://umpir.ump.edu.my/id/eprint/26228/ Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP Fahmi, Samsuri Alkaisi, M. M. Evans, J. J. TK Electrical engineering. Electronics Nuclear engineering A Biochip is a platform of miniaturized microarrays arranged on a solid substrate that permits many tests to be performed at the same time in order to achieve higher throughput and speed. Single-cell identification, imaging and analysis in situ using Atomic Force Microscopy (AFM) faced a lot of difficulties as immobilizing the cells for direct cellular analysis is still a major challenge. One approach to overcome this problem was the use of organized arrangements like microarrays to trap the cells and placed them in a controlled manner. This can be achieved by fabricating these microarrays on a special platform such as Biochip. To manipulate the cells' movements, an electrokinetic phenomenon known as dielectrophoresis (DEP) was used for that purpose. Non-uniform AC electric fields generated by the interdigitated microelectrode arrays provide an ideal method for manipulating and controlling particles. The cells were captured using positive and negative DEP forces in cavities placed at different locations within the electrode arrays. As some cells have no tendency to spread over substrates during culturing, the contact area between the cell and substrate is very small, often leading to cell detachment by the scanning tip. Thus by employing the cavity trapping method, not only the cells were perfectly anchored to the surface but also their heights were lowered to within a set-scan level of the AFM, enabling faster time-to-analysis. This paper reports the development of a new layout for the Biochip electrodes intended for DEP cell manipulations. IEEE 2011 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26228/1/Biochip%20development%20using%20Nanoimprint%20Lithography%20%28NIL%29%20and%20metallic%20.pdf Fahmi, Samsuri and Alkaisi, M. M. and Evans, J. J. (2011) Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP. In: International Conference on Electrical, Control and Computer Engineering 2011 (InECCE 2011)., 21-22 June 2011 , Hyatt Regency, Kuantan, Pahang, Malaysia. pp. 382-387.. ISBN 978-1-61284-229-5 https://doi.org/10.1109/INECCE.2011.5953911
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TK Electrical engineering. Electronics Nuclear engineering
spellingShingle TK Electrical engineering. Electronics Nuclear engineering
Fahmi, Samsuri
Alkaisi, M. M.
Evans, J. J.
Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP
description A Biochip is a platform of miniaturized microarrays arranged on a solid substrate that permits many tests to be performed at the same time in order to achieve higher throughput and speed. Single-cell identification, imaging and analysis in situ using Atomic Force Microscopy (AFM) faced a lot of difficulties as immobilizing the cells for direct cellular analysis is still a major challenge. One approach to overcome this problem was the use of organized arrangements like microarrays to trap the cells and placed them in a controlled manner. This can be achieved by fabricating these microarrays on a special platform such as Biochip. To manipulate the cells' movements, an electrokinetic phenomenon known as dielectrophoresis (DEP) was used for that purpose. Non-uniform AC electric fields generated by the interdigitated microelectrode arrays provide an ideal method for manipulating and controlling particles. The cells were captured using positive and negative DEP forces in cavities placed at different locations within the electrode arrays. As some cells have no tendency to spread over substrates during culturing, the contact area between the cell and substrate is very small, often leading to cell detachment by the scanning tip. Thus by employing the cavity trapping method, not only the cells were perfectly anchored to the surface but also their heights were lowered to within a set-scan level of the AFM, enabling faster time-to-analysis. This paper reports the development of a new layout for the Biochip electrodes intended for DEP cell manipulations.
format Conference or Workshop Item
author Fahmi, Samsuri
Alkaisi, M. M.
Evans, J. J.
author_facet Fahmi, Samsuri
Alkaisi, M. M.
Evans, J. J.
author_sort Fahmi, Samsuri
title Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP
title_short Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP
title_full Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP
title_fullStr Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP
title_full_unstemmed Biochip development using Nanoimprint Lithography (NIL) and metallic thermal evaporation techniques for biological cells manipulation using DEP
title_sort biochip development using nanoimprint lithography (nil) and metallic thermal evaporation techniques for biological cells manipulation using dep
publisher IEEE
publishDate 2011
url http://umpir.ump.edu.my/id/eprint/26228/
http://umpir.ump.edu.my/id/eprint/26228/
http://umpir.ump.edu.my/id/eprint/26228/1/Biochip%20development%20using%20Nanoimprint%20Lithography%20%28NIL%29%20and%20metallic%20.pdf
first_indexed 2023-09-18T22:40:43Z
last_indexed 2023-09-18T22:40:43Z
_version_ 1777416896156532736

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