Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool

Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool

Fenugreek is a multiregional plant mostly found in Asia, the Middle East, and some European countries. Thus, this study aimed to optimize the microwave-assisted extraction (MAE) process parameters such as irradiation time (2–4 min), ethanol concentration (40–80%), microwave power (500–700 W), solid-...

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Main Authors: Akbari, Sweeta, Nour, A. H., R. M., Yunus
Format: Article
Language:English
Published: Elsevier Masson SAS 2019
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/26647/
http://umpir.ump.edu.my/id/eprint/26647/
http://umpir.ump.edu.my/id/eprint/26647/
http://umpir.ump.edu.my/id/eprint/26647/1/Optimization%20of%20saponins1.pdf
id ump-26647
recordtype eprints
spelling ump-266472019-11-28T04:43:06Z http://umpir.ump.edu.my/id/eprint/26647/ Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool Akbari, Sweeta Nour, A. H. R. M., Yunus TP Chemical technology Fenugreek is a multiregional plant mostly found in Asia, the Middle East, and some European countries. Thus, this study aimed to optimize the microwave-assisted extraction (MAE) process parameters such as irradiation time (2–4 min), ethanol concentration (40–80%), microwave power (500–700 W), solid-to-liquid ratio (1:8–1:12 g/mL), and a fixed extraction temperature (70 °C) for fenugreek seed powder. Response surface methodology (RSM) was applied to optimize MAE process parameters to achieve optimal total saponin content (TSC), total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant capacities of fenugreek seed extract. The levels of independent variables were first evaluated using one-factor-at-one-time method, after which the suitable levels (−1, 0, and +1) were selected for the optimization process. The optimal conditions of MAE parameters were found to be 2.84 min, 572.50 W, 63.68%, and 0.09 g/mL. Based on the suggested optimum conditions, the obtained responses were 195.89 ± 1.07 (mg diosgenin equivalent/g d.w.), 81.85 ± 0.61 (mg gallic acid equivalent/g d.w.), 92.86 ± 0.56%, and 95.85 ± 0.81%. Analysis of variance revealed that all the responses including TSC, TPC, DPPH, and ABTS antioxidant capacities were significantly influenced (P < 0.05) by irradiation time, solvent concentration, microwave oven power, and feed-to-solvent ratio. Hence, the results obtained from liquid chromatography–quadrupole–time-of-flight mass spectrometry indicated the presence of steroid saponins, triterpenoid saponins, phenolics, and other natural antioxidants. Elsevier Masson SAS 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26647/1/Optimization%20of%20saponins1.pdf Akbari, Sweeta and Nour, A. H. and R. M., Yunus (2019) Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool. Comptes Rendus Chimie. pp. 1-14. ISSN 1631-0748 (In Press) https://doi.org/10.1016/j.crci.2019.07.007 https://doi.org/10.1016/j.crci.2019.07.007
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Akbari, Sweeta
Nour, A. H.
R. M., Yunus
Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool
description Fenugreek is a multiregional plant mostly found in Asia, the Middle East, and some European countries. Thus, this study aimed to optimize the microwave-assisted extraction (MAE) process parameters such as irradiation time (2–4 min), ethanol concentration (40–80%), microwave power (500–700 W), solid-to-liquid ratio (1:8–1:12 g/mL), and a fixed extraction temperature (70 °C) for fenugreek seed powder. Response surface methodology (RSM) was applied to optimize MAE process parameters to achieve optimal total saponin content (TSC), total phenolic content (TPC), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) antioxidant capacities of fenugreek seed extract. The levels of independent variables were first evaluated using one-factor-at-one-time method, after which the suitable levels (−1, 0, and +1) were selected for the optimization process. The optimal conditions of MAE parameters were found to be 2.84 min, 572.50 W, 63.68%, and 0.09 g/mL. Based on the suggested optimum conditions, the obtained responses were 195.89 ± 1.07 (mg diosgenin equivalent/g d.w.), 81.85 ± 0.61 (mg gallic acid equivalent/g d.w.), 92.86 ± 0.56%, and 95.85 ± 0.81%. Analysis of variance revealed that all the responses including TSC, TPC, DPPH, and ABTS antioxidant capacities were significantly influenced (P < 0.05) by irradiation time, solvent concentration, microwave oven power, and feed-to-solvent ratio. Hence, the results obtained from liquid chromatography–quadrupole–time-of-flight mass spectrometry indicated the presence of steroid saponins, triterpenoid saponins, phenolics, and other natural antioxidants.
format Article
author Akbari, Sweeta
Nour, A. H.
R. M., Yunus
author_facet Akbari, Sweeta
Nour, A. H.
R. M., Yunus
author_sort Akbari, Sweeta
title Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool
title_short Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool
title_full Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool
title_fullStr Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool
title_full_unstemmed Optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool
title_sort optimization of saponins, phenolics, and antioxidants extracted from fenugreek seeds using microwave-assisted extraction and response surface methodology as an optimizing tool
publisher Elsevier Masson SAS
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/26647/
http://umpir.ump.edu.my/id/eprint/26647/
http://umpir.ump.edu.my/id/eprint/26647/
http://umpir.ump.edu.my/id/eprint/26647/1/Optimization%20of%20saponins1.pdf
first_indexed 2023-09-18T22:41:36Z
last_indexed 2023-09-18T22:41:36Z
_version_ 1777416952313020416

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