Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase

Biocatalysis is a powerful tool for organic synthesis, especially for the synthesis of high value products such as chiral molecules and intermediates. Chiral amines can be synthesized by kinetic resolution of racemic amines or by the asymmetric synthesis from prochiral ketones. Kinetic parameter est...

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Main Authors:	S. D., Vijaya Kumar, Zainatul Bahiyah, Handani, Rohana, Abu
Format:	Conference or Workshop Item
Language:	English English
Published:	2019
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/26906/ http://umpir.ump.edu.my/id/eprint/26906/1/88.%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf http://umpir.ump.edu.my/id/eprint/26906/2/88.1%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf

id	ump-26906
recordtype	eprints
spelling	ump-269062020-03-18T02:29:02Z http://umpir.ump.edu.my/id/eprint/26906/ Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase S. D., Vijaya Kumar Zainatul Bahiyah, Handani Rohana, Abu TP Chemical technology Biocatalysis is a powerful tool for organic synthesis, especially for the synthesis of high value products such as chiral molecules and intermediates. Chiral amines can be synthesized by kinetic resolution of racemic amines or by the asymmetric synthesis from prochiral ketones. Kinetic parameter estimation of such biocatalytic reaction is useful to evaluate process and technology options. In this research, the Michaelis–Menten kinetic parameters from the asymmetric synthesis of (R)-1-phenylethylamine from acetophenone and alanine with immobilized ω-transaminase were estimated. The immobilized ω-transaminase was prepared by entrapment with diaion beads. The kinetic parameters such as, Michaelis-Menten constant (Km) and maximum rate of reaction (Vmax) were measured using initial rate experiments by varying the substrate acetophenone concentrations (2 mM to 10 mM) at 100 mM of amino donor. The kinetic parameters were then estimated by Lineweaver-burk analysis. The Vmax and Km was estimated at 6.33 mM/min and 0.382 mM, respectively. 2019 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/26906/1/88.%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf pdf en http://umpir.ump.edu.my/id/eprint/26906/2/88.1%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf S. D., Vijaya Kumar and Zainatul Bahiyah, Handani and Rohana, Abu (2019) Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase. In: Energy Security and Chemical Engineering Congress (ESCHE2019), 17-19 July 2019 , Parkroyal Resort Penang, Malaysia. pp. 1-4.. (Unpublished)
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TP Chemical technology
spellingShingle	TP Chemical technology S. D., Vijaya Kumar Zainatul Bahiyah, Handani Rohana, Abu Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase
description	Biocatalysis is a powerful tool for organic synthesis, especially for the synthesis of high value products such as chiral molecules and intermediates. Chiral amines can be synthesized by kinetic resolution of racemic amines or by the asymmetric synthesis from prochiral ketones. Kinetic parameter estimation of such biocatalytic reaction is useful to evaluate process and technology options. In this research, the Michaelis–Menten kinetic parameters from the asymmetric synthesis of (R)-1-phenylethylamine from acetophenone and alanine with immobilized ω-transaminase were estimated. The immobilized ω-transaminase was prepared by entrapment with diaion beads. The kinetic parameters such as, Michaelis-Menten constant (Km) and maximum rate of reaction (Vmax) were measured using initial rate experiments by varying the substrate acetophenone concentrations (2 mM to 10 mM) at 100 mM of amino donor. The kinetic parameters were then estimated by Lineweaver-burk analysis. The Vmax and Km was estimated at 6.33 mM/min and 0.382 mM, respectively.
format	Conference or Workshop Item
author	S. D., Vijaya Kumar Zainatul Bahiyah, Handani Rohana, Abu
author_facet	S. D., Vijaya Kumar Zainatul Bahiyah, Handani Rohana, Abu
author_sort	S. D., Vijaya Kumar
title	Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase
title_short	Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase
title_full	Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase
title_fullStr	Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase
title_full_unstemmed	Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase
title_sort	kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/26906/ http://umpir.ump.edu.my/id/eprint/26906/1/88.%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf http://umpir.ump.edu.my/id/eprint/26906/2/88.1%20Kinetic%20study%20of%20asymmetric%20synthesis%20of%20chiral.pdf
first_indexed	2023-09-18T22:42:11Z
last_indexed	2023-09-18T22:42:11Z
_version_	1777416988812902400

Kinetic study of asymmetric synthesis of chiral amine with immobilized transaminase

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