Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC)

Stevia rebaudiana leaves known as sweet leaf and it contain with non-caloric sweeteners (steviol-glycosides) whose consumption could exert beneficial effects on human health. In Malaysia, the applicable of steviol-glycosides was very limited. The sweetness of steviol-glycosides was about 300 times m...

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Main Author:	Nurlin, Abu Samah
Format:	Article
Language:	English
Published:	World Academy of Reseacrh and Publication 2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/2725/ http://umpir.ump.edu.my/id/eprint/2725/1/nurlin.doc

id	ump-2725
recordtype	eprints
spelling	ump-27252018-06-28T08:42:56Z http://umpir.ump.edu.my/id/eprint/2725/ Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC) Nurlin, Abu Samah TP Chemical technology Stevia rebaudiana leaves known as sweet leaf and it contain with non-caloric sweeteners (steviol-glycosides) whose consumption could exert beneficial effects on human health. In Malaysia, the applicable of steviol-glycosides was very limited. The sweetness of steviol-glycosides was about 300 times more than sugar produce from sugar cane. Nowadays, sugarcane has critical value due to its high consumptions in community. The objective of this study is to determine stevioside and rebaudioside A in Stevia Rebaudiana leaves qualitatively by using soxhlet extraction method analyzed via preparative HPLC. A series of standard solution for both stevioside and rebaudioside A were analyzed. Samples were sequentially extracted using methanol as the solvent and the column had been used in this study was Waters XBridge C18 column (150 mm x 4.6 mm I.D., 5µm). The mobile phase for C18 column performed in isocratic mode elution consisting of acetonitrile:water (80:20,v/v). Stevioside were the most abundant steviol-glycosides (Rt = 4.23 min) found in stevia rebaudiana leaves samples followed by rebaudioside A (Rt = 4.28 min). The objective of analysis was achieved and this leads to the suggestion of using the Capcell Pak C18 – MGII Column (250 mm x 4.60 mm ID, 5 µm) as the replacement to the C18 column that was used in this study. Also, it is a good practice if the sample was extracted using solid phase extraction method compare to the conventional practice using sohxlet extraction method. World Academy of Reseacrh and Publication 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2725/1/nurlin.doc Nurlin, Abu Samah (2012) Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC). International Journal of Chemical and Environmental Engineering. ISSN 2078-0737
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Nurlin, Abu Samah Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC)
description	Stevia rebaudiana leaves known as sweet leaf and it contain with non-caloric sweeteners (steviol-glycosides) whose consumption could exert beneficial effects on human health. In Malaysia, the applicable of steviol-glycosides was very limited. The sweetness of steviol-glycosides was about 300 times more than sugar produce from sugar cane. Nowadays, sugarcane has critical value due to its high consumptions in community. The objective of this study is to determine stevioside and rebaudioside A in Stevia Rebaudiana leaves qualitatively by using soxhlet extraction method analyzed via preparative HPLC. A series of standard solution for both stevioside and rebaudioside A were analyzed. Samples were sequentially extracted using methanol as the solvent and the column had been used in this study was Waters XBridge C18 column (150 mm x 4.6 mm I.D., 5µm). The mobile phase for C18 column performed in isocratic mode elution consisting of acetonitrile:water (80:20,v/v). Stevioside were the most abundant steviol-glycosides (Rt = 4.23 min) found in stevia rebaudiana leaves samples followed by rebaudioside A (Rt = 4.28 min). The objective of analysis was achieved and this leads to the suggestion of using the Capcell Pak C18 – MGII Column (250 mm x 4.60 mm ID, 5 µm) as the replacement to the C18 column that was used in this study. Also, it is a good practice if the sample was extracted using solid phase extraction method compare to the conventional practice using sohxlet extraction method.
format	Article
author	Nurlin, Abu Samah
author_facet	Nurlin, Abu Samah
author_sort	Nurlin, Abu Samah
title	Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC)
title_short	Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC)
title_full	Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC)
title_fullStr	Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC)
title_full_unstemmed	Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC)
title_sort	determination of stevioside and rebaudioside a in stevia rebaudiana leaves via preparative high performance liquid chromatography (prep-hplc)
publisher	World Academy of Reseacrh and Publication
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/2725/ http://umpir.ump.edu.my/id/eprint/2725/1/nurlin.doc
first_indexed	2023-09-18T21:56:47Z
last_indexed	2023-09-18T21:56:47Z
_version_	1777414132575764480

Determination of Stevioside and Rebaudioside A in Stevia Rebaudiana Leaves via preparative High Performance Liquid Chromatography (prep-HPLC)

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