Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2

Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain...

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Main Authors:	Wei, Cheng Pang, Aizi Nor Mazila, Ramli, Azzmer Azzar, Abdul Hamid
Format:	Article
Language:	English
Published:	Trans Tech Publications 2020
Subjects:	QD Chemistry
Online Access:	http://umpir.ump.edu.my/id/eprint/28150/ http://umpir.ump.edu.my/id/eprint/28150/ http://umpir.ump.edu.my/id/eprint/28150/ http://umpir.ump.edu.my/id/eprint/28150/1/Gene%20Expression%20Analysis%20of%20Fruit%20Bromelain1.pdf

id	ump-28150
recordtype	eprints
spelling	ump-281502020-03-18T01:44:05Z http://umpir.ump.edu.my/id/eprint/28150/ Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2 Wei, Cheng Pang Aizi Nor Mazila, Ramli Azzmer Azzar, Abdul Hamid QD Chemistry Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain during the ripening stage of A. comosus cultivar MD 2. The gene expression of fruit bromelain was investigated via relative gene expression analysis using qPCR while the proteolytic activity of fruit bromelain was analysed via enzymatic assay using casein as a substrate. The qPCR analysis revealed that the expression of fruit bromelain was down-regulated 10-fold in ripe pineapple fruits. Besides that, the unripe pineapple fruits [1.9101 ± 0.0831 U/mL] had a higher proteolytic activity than the ripe MD 2 pineapple fruits [1.1333 ± 0.0896 U/mL]. This result showed that the function of fruit bromelain may be related to the protection of young pineapple fruits during the fruit development stage Trans Tech Publications 2020 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/28150/1/Gene%20Expression%20Analysis%20of%20Fruit%20Bromelain1.pdf Wei, Cheng Pang and Aizi Nor Mazila, Ramli and Azzmer Azzar, Abdul Hamid (2020) Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2. Materials Science Forum, 981. pp. 209-214. ISSN 0255-5476 https://doi.org/10.4028/www.scientific.net/MSF.981.209 https://doi.org/10.4028/www.scientific.net/MSF.981.209
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Wei, Cheng Pang Aizi Nor Mazila, Ramli Azzmer Azzar, Abdul Hamid Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2
description	Fruit bromelain is a proteolytic enzyme harbouring cysteine catalytic residue found abundantly in pineapple fruit. The expression of cysteine proteases is usually regulated during fruit ripening. In the present study, we aimed to study the expression and proteolytic activity level of fruit bromelain during the ripening stage of A. comosus cultivar MD 2. The gene expression of fruit bromelain was investigated via relative gene expression analysis using qPCR while the proteolytic activity of fruit bromelain was analysed via enzymatic assay using casein as a substrate. The qPCR analysis revealed that the expression of fruit bromelain was down-regulated 10-fold in ripe pineapple fruits. Besides that, the unripe pineapple fruits [1.9101 ± 0.0831 U/mL] had a higher proteolytic activity than the ripe MD 2 pineapple fruits [1.1333 ± 0.0896 U/mL]. This result showed that the function of fruit bromelain may be related to the protection of young pineapple fruits during the fruit development stage
format	Article
author	Wei, Cheng Pang Aizi Nor Mazila, Ramli Azzmer Azzar, Abdul Hamid
author_facet	Wei, Cheng Pang Aizi Nor Mazila, Ramli Azzmer Azzar, Abdul Hamid
author_sort	Wei, Cheng Pang
title	Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2
title_short	Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2
title_full	Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2
title_fullStr	Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2
title_full_unstemmed	Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2
title_sort	gene expression analysis of fruit bromelain in ripening of ananas comosus cultivar md 2
publisher	Trans Tech Publications
publishDate	2020
url	http://umpir.ump.edu.my/id/eprint/28150/ http://umpir.ump.edu.my/id/eprint/28150/ http://umpir.ump.edu.my/id/eprint/28150/ http://umpir.ump.edu.my/id/eprint/28150/1/Gene%20Expression%20Analysis%20of%20Fruit%20Bromelain1.pdf
first_indexed	2023-09-18T22:44:05Z
last_indexed	2023-09-18T22:44:05Z
_version_	1777417108020264960

Gene Expression Analysis of Fruit Bromelain in Ripening of Ananas comosus Cultivar MD 2

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