Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition

Anaerobic fermentation is a feasible option and a low-cost process for production of acetic acid. However, if the material used for production is hardly degradable such as lignocelluloses waste, the process does need the bacteria that are versatile in order to produce a high amount of yield and degr...

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Main Authors:	Mohd Faizan, Jamaluddin, N., Zainol
Format:	Article
Language:	English
Published:	World Academy of Reseacrh and Publication 2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/2888/ http://umpir.ump.edu.my/id/eprint/2888/ http://umpir.ump.edu.my/id/eprint/2888/1/Isolation_and_identification_of_acetic_acid_producer_from_mixed_culture_of_soil_and_banana_stem_waste_in_anaerobic_condition._%29.pdf

id	ump-2888
recordtype	eprints
spelling	ump-28882018-01-15T02:27:48Z http://umpir.ump.edu.my/id/eprint/2888/ Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition Mohd Faizan, Jamaluddin N., Zainol TP Chemical technology Anaerobic fermentation is a feasible option and a low-cost process for production of acetic acid. However, if the material used for production is hardly degradable such as lignocelluloses waste, the process does need the bacteria that are versatile in order to produce a high amount of yield and degrade combination of substrate. Banana stem waste is lignocellulosic waste, and it contained high amount of cellulose that can be used as a substrate for acetic acid production. Meanwhile, mixed culture from soil did contain high amount and variety species of bacteria. In order to increase the yield and find the suitable bacteria for the process, the pure strain needs to be obtained. Therefore, isolation and identification of bacteria need to be done. Isolation and identification of acetic acid producer from mixed culture of soil and banana stem waste was done. Isolation was done by aseptically serial diluted. Culture from each dilution will be cultured anaerobically towards selective medium that consists of glucose. 15 strains from 57 strains isolated able to utilize glucose to produce acetic acid. From 15 strains, one strain able to produce acetic acid up to 1.8 g/l and can degrade lignin. This strain was chosen to be identified using gram stain, biochemical test using BIOLOG kit. From the identification results, the strain was known as Enterobacter Soli. This strain can degrade a variety of substrate, including lignin, cellulose and almost all types of re- ducing sugar (C5-C6) and produce a high amount of acetic acid by using complex raw material as lignocellulosic waste. World Academy of Reseacrh and Publication 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2888/1/Isolation_and_identification_of_acetic_acid_producer_from_mixed_culture_of_soil_and_banana_stem_waste_in_anaerobic_condition._%29.pdf Mohd Faizan, Jamaluddin and N., Zainol (2012) Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition. International Journal of Chemical and Environmental Engineering, 3 (2). pp. 15-18. ISSN 2078-0737 http://warponline.org/uploads/contents/12-content-Isolation-and-identification-of-acetic-acid-producer-from-mixed-culture-of-soil-and-banana-stem-waste-in-anaerobic-condition..pdf
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mohd Faizan, Jamaluddin N., Zainol Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition
description	Anaerobic fermentation is a feasible option and a low-cost process for production of acetic acid. However, if the material used for production is hardly degradable such as lignocelluloses waste, the process does need the bacteria that are versatile in order to produce a high amount of yield and degrade combination of substrate. Banana stem waste is lignocellulosic waste, and it contained high amount of cellulose that can be used as a substrate for acetic acid production. Meanwhile, mixed culture from soil did contain high amount and variety species of bacteria. In order to increase the yield and find the suitable bacteria for the process, the pure strain needs to be obtained. Therefore, isolation and identification of bacteria need to be done. Isolation and identification of acetic acid producer from mixed culture of soil and banana stem waste was done. Isolation was done by aseptically serial diluted. Culture from each dilution will be cultured anaerobically towards selective medium that consists of glucose. 15 strains from 57 strains isolated able to utilize glucose to produce acetic acid. From 15 strains, one strain able to produce acetic acid up to 1.8 g/l and can degrade lignin. This strain was chosen to be identified using gram stain, biochemical test using BIOLOG kit. From the identification results, the strain was known as Enterobacter Soli. This strain can degrade a variety of substrate, including lignin, cellulose and almost all types of re- ducing sugar (C5-C6) and produce a high amount of acetic acid by using complex raw material as lignocellulosic waste.
format	Article
author	Mohd Faizan, Jamaluddin N., Zainol
author_facet	Mohd Faizan, Jamaluddin N., Zainol
author_sort	Mohd Faizan, Jamaluddin
title	Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition
title_short	Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition
title_full	Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition
title_fullStr	Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition
title_full_unstemmed	Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition
title_sort	isolation and identification of acetic acid producer from mixed culture of soil and banana stem waste in anaerobic condition
publisher	World Academy of Reseacrh and Publication
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/2888/ http://umpir.ump.edu.my/id/eprint/2888/ http://umpir.ump.edu.my/id/eprint/2888/1/Isolation_and_identification_of_acetic_acid_producer_from_mixed_culture_of_soil_and_banana_stem_waste_in_anaerobic_condition._%29.pdf
first_indexed	2023-09-18T21:56:52Z
last_indexed	2023-09-18T21:56:52Z
_version_	1777414137214664704

Isolation and Identification of Acetic Acid Producer from Mixed Culture of Soil and Banana Stem Waste in Anaerobic Condition

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