Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae

Abstract. Dengue, yellow fever and dengue haemorrhagic fever are health problems in many countries. The Aedesaegypti mosquito is the major vector for these diseases. The development of insect resistance and side effects associated with synthetic pesticides make essential oils the focus of intense r...

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Main Authors:	Azhari, H. Nour, Nour, A. H., M. M., Yusoff, Jessinta, D/O Sandanasamy
Format:	Conference or Workshop Item
Language:	English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3083/ http://umpir.ump.edu.my/id/eprint/3083/1/ICBEC2012-Bioactive_compounds_from_essential_oil.pdf

id	ump-3083
recordtype	eprints
spelling	ump-30832018-03-08T06:11:31Z http://umpir.ump.edu.my/id/eprint/3083/ Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae Azhari, H. Nour Nour, A. H. M. M., Yusoff Jessinta, D/O Sandanasamy TP Chemical technology Abstract. Dengue, yellow fever and dengue haemorrhagic fever are health problems in many countries. The Aedesaegypti mosquito is the major vector for these diseases. The development of insect resistance and side effects associated with synthetic pesticides make essential oils the focus of intense research efforts because of their biological activities and long, safe use as natural products. In this study, the essential oils from two basil (O. basilicum) accessions were tested in a laboratory bioassay for larvicidal activity against third instar A. aegypti larvae. The essential oils were extracted by steam distillation, and their chemical compositions were determined by GC-MS. Approximately 13 compounds (>1%) were detected; most compounds were oxygenated monoterpenes. Methyl chavicol and geranial were the predominant compounds in the two observed accessions. For the bioassay, six concentrations (50, 100, 200, 300, 400 and 500 µg/ml) of essential oil solutions were prepared from the two basil accessions. For each concentration of oil solution, 25 third instar larvae were inserted. Controls, 1% DMSO or untreated larvae in tap water were also performed for comparison. The active ingredients in the essential oils were separated and identified by TLC and GC-MS. The larval assays provided LC50 and LC90 values for the methyl chavicol accession (MCV) were 160 and 262µg/ml, respectively; the values for the geranial-geraniol accession (GGV) were 174 and 356µg/ml, respectively. Linalool, geraniol, geranial, methyl chavicol and eugenol were active components against A. aegypti larvae. 2012 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3083/1/ICBEC2012-Bioactive_compounds_from_essential_oil.pdf Azhari, H. Nour and Nour, A. H. and M. M., Yusoff and Jessinta, D/O Sandanasamy (2012) Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae. In: 3rd International Conference on Biology, Environment and Chemistry, 24-25 November 2012 , Bangkok, Thailand. .
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Azhari, H. Nour Nour, A. H. M. M., Yusoff Jessinta, D/O Sandanasamy Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae
description	Abstract. Dengue, yellow fever and dengue haemorrhagic fever are health problems in many countries. The Aedesaegypti mosquito is the major vector for these diseases. The development of insect resistance and side effects associated with synthetic pesticides make essential oils the focus of intense research efforts because of their biological activities and long, safe use as natural products. In this study, the essential oils from two basil (O. basilicum) accessions were tested in a laboratory bioassay for larvicidal activity against third instar A. aegypti larvae. The essential oils were extracted by steam distillation, and their chemical compositions were determined by GC-MS. Approximately 13 compounds (>1%) were detected; most compounds were oxygenated monoterpenes. Methyl chavicol and geranial were the predominant compounds in the two observed accessions. For the bioassay, six concentrations (50, 100, 200, 300, 400 and 500 µg/ml) of essential oil solutions were prepared from the two basil accessions. For each concentration of oil solution, 25 third instar larvae were inserted. Controls, 1% DMSO or untreated larvae in tap water were also performed for comparison. The active ingredients in the essential oils were separated and identified by TLC and GC-MS. The larval assays provided LC50 and LC90 values for the methyl chavicol accession (MCV) were 160 and 262µg/ml, respectively; the values for the geranial-geraniol accession (GGV) were 174 and 356µg/ml, respectively. Linalool, geraniol, geranial, methyl chavicol and eugenol were active components against A. aegypti larvae.
format	Conference or Workshop Item
author	Azhari, H. Nour Nour, A. H. M. M., Yusoff Jessinta, D/O Sandanasamy
author_facet	Azhari, H. Nour Nour, A. H. M. M., Yusoff Jessinta, D/O Sandanasamy
author_sort	Azhari, H. Nour
title	Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae
title_short	Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae
title_full	Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae
title_fullStr	Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae
title_full_unstemmed	Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae
title_sort	bioactive compounds from basil (ocimumbasilicum) essential oils with larvicidal activity against aedesaegypti larvae
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/3083/ http://umpir.ump.edu.my/id/eprint/3083/1/ICBEC2012-Bioactive_compounds_from_essential_oil.pdf
first_indexed	2023-09-18T21:57:07Z
last_indexed	2023-09-18T21:57:07Z
_version_	1777414152806989824

Bioactive Compounds from Basil (Ocimumbasilicum) Essential Oils with Larvicidal Activity against Aedesaegypti Larvae

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