Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum

Biofuels production through fermentation from renewable waste has been put more to focus in mid 20th century till now. Main focus of the research is to determine optimum fermentation time to obtain maximum concentration of butanol and ethanol via ABE fermentation. The ABE fermentation is a two phase...

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Main Author:	Muhammad Zaidi, Othman
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3248/ http://umpir.ump.edu.my/id/eprint/3248/ http://umpir.ump.edu.my/id/eprint/3248/1/CD5820_MUHAMMAD_ZAIDI.pdf

id	ump-3248
recordtype	eprints
spelling	ump-32482015-03-03T08:00:09Z http://umpir.ump.edu.my/id/eprint/3248/ Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum Muhammad Zaidi, Othman TP Chemical technology Biofuels production through fermentation from renewable waste has been put more to focus in mid 20th century till now. Main focus of the research is to determine optimum fermentation time to obtain maximum concentration of butanol and ethanol via ABE fermentation. The ABE fermentation is a two phase chemical production which is from acidogenesis into solventogenesis. The fermentation is caused by clostridia bacteria and in this case of study is Clostridium acetobutylicum pattered NCIMB 13357. The main substrate for nutrient in this study is Palm Oil Mill Effluent (POME) which freshly taken from Lepar Hilir, Pahang. Through the research, POME was found to contain majorly of pentose and carbohydrate substance such as galactose, glucose, fructose, sucrose and lactose. The research parameter is fermentation time at 72, 80, 48 and 60 hours. The experiments start from medium preparation, cultivation of bacteria, inoculation, fermentation and lastly analysis of product. All standard solution is made in order to standardize the reading of concentration of product and substance analyzed. The condition for bacteria environment is set to anaerobic and optimum pH at 5.8, temperature at 37 ⁰C and agitation speed is set at 200 rpm along ABE fermentation. Final result shown that at fermentation time of 40 hours, the maximum value of 0.07 g/L of butanol was produced, while for ethanol is 83.499 g/L at 20 hour fermentation time. POME is encouragingly valid for use as fermentation media as it contain many utilizable substrates for Clostridium acetobutylicum. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3248/1/CD5820_MUHAMMAD_ZAIDI.pdf Muhammad Zaidi, Othman (2010) Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang . http://iportal.ump.edu.my/lib/item?id=chamo:58770&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Muhammad Zaidi, Othman Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum
description	Biofuels production through fermentation from renewable waste has been put more to focus in mid 20th century till now. Main focus of the research is to determine optimum fermentation time to obtain maximum concentration of butanol and ethanol via ABE fermentation. The ABE fermentation is a two phase chemical production which is from acidogenesis into solventogenesis. The fermentation is caused by clostridia bacteria and in this case of study is Clostridium acetobutylicum pattered NCIMB 13357. The main substrate for nutrient in this study is Palm Oil Mill Effluent (POME) which freshly taken from Lepar Hilir, Pahang. Through the research, POME was found to contain majorly of pentose and carbohydrate substance such as galactose, glucose, fructose, sucrose and lactose. The research parameter is fermentation time at 72, 80, 48 and 60 hours. The experiments start from medium preparation, cultivation of bacteria, inoculation, fermentation and lastly analysis of product. All standard solution is made in order to standardize the reading of concentration of product and substance analyzed. The condition for bacteria environment is set to anaerobic and optimum pH at 5.8, temperature at 37 ⁰C and agitation speed is set at 200 rpm along ABE fermentation. Final result shown that at fermentation time of 40 hours, the maximum value of 0.07 g/L of butanol was produced, while for ethanol is 83.499 g/L at 20 hour fermentation time. POME is encouragingly valid for use as fermentation media as it contain many utilizable substrates for Clostridium acetobutylicum.
format	Undergraduates Project Papers
author	Muhammad Zaidi, Othman
author_facet	Muhammad Zaidi, Othman
author_sort	Muhammad Zaidi, Othman
title	Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum
title_short	Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum
title_full	Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum
title_fullStr	Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum
title_full_unstemmed	Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum
title_sort	effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/3248/ http://umpir.ump.edu.my/id/eprint/3248/ http://umpir.ump.edu.my/id/eprint/3248/1/CD5820_MUHAMMAD_ZAIDI.pdf
first_indexed	2023-09-18T21:57:22Z
last_indexed	2023-09-18T21:57:22Z
_version_	1777414169284313088

Effect of fermentation time on butanol and ethanol production from palm mill oil effluent by clostridium acetobutylicum

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