Whey protein fraction based on Q-sepharose anion exchange chromatography

Whey proteinsarewell known for their nutritional value and had versatile functional properties.The mains proteins present in whey are α-lactalbumin, β-lactoglobulin, immunoglobulin andbovin eserum albumin. In this study, whey protein component is separate into several fractions using Q-Sepharose ani...

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Main Author: Zianor Azrina, Z. A.
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/3283/
http://umpir.ump.edu.my/id/eprint/3283/
http://umpir.ump.edu.my/id/eprint/3283/1/CD5505_ZIANOR_AZLINA.pdf
id ump-3283
recordtype eprints
spelling ump-32832018-10-17T08:17:43Z http://umpir.ump.edu.my/id/eprint/3283/ Whey protein fraction based on Q-sepharose anion exchange chromatography Zianor Azrina, Z. A. TP Chemical technology Whey proteinsarewell known for their nutritional value and had versatile functional properties.The mains proteins present in whey are α-lactalbumin, β-lactoglobulin, immunoglobulin andbovin eserum albumin. In this study, whey protein component is separate into several fractions using Q-Sepharose anion exchange chromatography column. The chromatographic process was run at different pH range from pH5 to pH8. The best separation was achieved at pH6 in which the major protein component can be separated into their individual protein fraction especially contains α- lactalbumin and β-lactoglobulin. The yield of α-lactalbumin and β-lactoglobulin recovered from 2 ml whey at pH 6 was 46% and 98.4% respectively. Fractionation of single and pure protein component from whey can add the value of the wheybased product and these proteins can be used for specific application. 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3283/1/CD5505_ZIANOR_AZLINA.pdf Zianor Azrina, Z. A. (2010) Whey protein fraction based on Q-sepharose anion exchange chromatography. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:57155&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Zianor Azrina, Z. A.
Whey protein fraction based on Q-sepharose anion exchange chromatography
description Whey proteinsarewell known for their nutritional value and had versatile functional properties.The mains proteins present in whey are α-lactalbumin, β-lactoglobulin, immunoglobulin andbovin eserum albumin. In this study, whey protein component is separate into several fractions using Q-Sepharose anion exchange chromatography column. The chromatographic process was run at different pH range from pH5 to pH8. The best separation was achieved at pH6 in which the major protein component can be separated into their individual protein fraction especially contains α- lactalbumin and β-lactoglobulin. The yield of α-lactalbumin and β-lactoglobulin recovered from 2 ml whey at pH 6 was 46% and 98.4% respectively. Fractionation of single and pure protein component from whey can add the value of the wheybased product and these proteins can be used for specific application.
format Undergraduates Project Papers
author Zianor Azrina, Z. A.
author_facet Zianor Azrina, Z. A.
author_sort Zianor Azrina, Z. A.
title Whey protein fraction based on Q-sepharose anion exchange chromatography
title_short Whey protein fraction based on Q-sepharose anion exchange chromatography
title_full Whey protein fraction based on Q-sepharose anion exchange chromatography
title_fullStr Whey protein fraction based on Q-sepharose anion exchange chromatography
title_full_unstemmed Whey protein fraction based on Q-sepharose anion exchange chromatography
title_sort whey protein fraction based on q-sepharose anion exchange chromatography
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/3283/
http://umpir.ump.edu.my/id/eprint/3283/
http://umpir.ump.edu.my/id/eprint/3283/1/CD5505_ZIANOR_AZLINA.pdf
first_indexed 2023-09-18T21:57:27Z
last_indexed 2023-09-18T21:57:27Z
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