The extraction of papain enzymes from papaya leaves

Due to importance of maximizing commercial value of papaya fruits, selection of papaya leaves as raw material in extracting papain enzymes is necessary to maintain the sales value of the fruit. The aim of the present study is to find out the best pre-treatment approach in extracting papain enzymes f...

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Main Author: Masita, Md Isa
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/3422/
http://umpir.ump.edu.my/id/eprint/3422/1/CD5947_MASITA_MAT_ISA_X.pdf
id ump-3422
recordtype eprints
spelling ump-34222015-03-03T08:01:04Z http://umpir.ump.edu.my/id/eprint/3422/ The extraction of papain enzymes from papaya leaves Masita, Md Isa TP Chemical technology Due to importance of maximizing commercial value of papaya fruits, selection of papaya leaves as raw material in extracting papain enzymes is necessary to maintain the sales value of the fruit. The aim of the present study is to find out the best pre-treatment approach in extracting papain enzymes from papaya leaves as well as to determine the optimum parameter for the extraction process. The leaves were subjected to a pre-treatment approach followed by hot water extraction process and then, enzymatic analysis. Among various pre-treatment approaches, maximum amino acids concentration, 0.15 mol/liter was achieved when grinding incorporated with ultrasonication was applied. The optimum period for extraction was 4 hours while optimum temperature was at 65°C. At this optimum condition, 0.45 mol/liter amino acids was achieved after grinding incorporated with ultrasonication sample undergo extraction process while 0.41 mol/liter amino acids was achieved after enzyme treatment incorporated with ultrasonication sample undergo extraction process. Utilization of HPLC is highly recommended to get the actual yield of papain enzymes being extracted without being declared as other substances. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3422/1/CD5947_MASITA_MAT_ISA_X.pdf Masita, Md Isa (2010) The extraction of papain enzymes from papaya leaves. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Masita, Md Isa
The extraction of papain enzymes from papaya leaves
description Due to importance of maximizing commercial value of papaya fruits, selection of papaya leaves as raw material in extracting papain enzymes is necessary to maintain the sales value of the fruit. The aim of the present study is to find out the best pre-treatment approach in extracting papain enzymes from papaya leaves as well as to determine the optimum parameter for the extraction process. The leaves were subjected to a pre-treatment approach followed by hot water extraction process and then, enzymatic analysis. Among various pre-treatment approaches, maximum amino acids concentration, 0.15 mol/liter was achieved when grinding incorporated with ultrasonication was applied. The optimum period for extraction was 4 hours while optimum temperature was at 65°C. At this optimum condition, 0.45 mol/liter amino acids was achieved after grinding incorporated with ultrasonication sample undergo extraction process while 0.41 mol/liter amino acids was achieved after enzyme treatment incorporated with ultrasonication sample undergo extraction process. Utilization of HPLC is highly recommended to get the actual yield of papain enzymes being extracted without being declared as other substances.
format Undergraduates Project Papers
author Masita, Md Isa
author_facet Masita, Md Isa
author_sort Masita, Md Isa
title The extraction of papain enzymes from papaya leaves
title_short The extraction of papain enzymes from papaya leaves
title_full The extraction of papain enzymes from papaya leaves
title_fullStr The extraction of papain enzymes from papaya leaves
title_full_unstemmed The extraction of papain enzymes from papaya leaves
title_sort extraction of papain enzymes from papaya leaves
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/3422/
http://umpir.ump.edu.my/id/eprint/3422/1/CD5947_MASITA_MAT_ISA_X.pdf
first_indexed 2023-09-18T21:57:43Z
last_indexed 2023-09-18T21:57:43Z
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