Optimization of pullulanase activity through design of experiment (DOE)

Enzyme has been used widely in industries today due to its capability in catalyzing various types of biochemical reactions. However, the high cost of enzyme gives problems to the enzyme industries. A research was done to optimize the pullulanase activity from Bacillus Acidopullulyticus using experim...

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Main Author:	Norsalita, Abd Talib
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3423/ http://umpir.ump.edu.my/id/eprint/3423/ http://umpir.ump.edu.my/id/eprint/3423/1/CD5948_NORSALITA_ABD_TALIB_X.pdf

id	ump-3423
recordtype	eprints
spelling	ump-34232015-03-03T08:01:04Z http://umpir.ump.edu.my/id/eprint/3423/ Optimization of pullulanase activity through design of experiment (DOE) Norsalita, Abd Talib TP Chemical technology Enzyme has been used widely in industries today due to its capability in catalyzing various types of biochemical reactions. However, the high cost of enzyme gives problems to the enzyme industries. A research was done to optimize the pullulanase activity from Bacillus Acidopullulyticus using experimental design. Two level Full Factorial design was applied in the screening process of four parameters for pullulanase activity and only three parameters were recognized as significant and effective factors for pullulanase activity. The three significant parameters were pH, enzyme and substrate concentration. Thus the three parameters were selected for further optimization studies using response surface methodology (RSM) in Design Expert version 7.1.6 software with Central Composite Design (CCD) mode. Thirty four sets of experiments with different parameter values were suggested. The predicted optimum values with R2 of 0.8729 for pH, enzyme concentration, substrate concentration and pullulanase activity were 6.60, 11.11 U/ml, 4.44% (w/v) and 3.17 U/ml respectively. One validation experiment was run using the optimized parameter and resulted 3.05 U/ml pullulanase activities as compared to the suggested values of 3.17 U/ml. As a conclusion, this research was successful to increase the pullulanase activity with a minimum numbers of experiments. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3423/1/CD5948_NORSALITA_ABD_TALIB_X.pdf Norsalita, Abd Talib (2010) Optimization of pullulanase activity through design of experiment (DOE). Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:58721&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Norsalita, Abd Talib Optimization of pullulanase activity through design of experiment (DOE)
description	Enzyme has been used widely in industries today due to its capability in catalyzing various types of biochemical reactions. However, the high cost of enzyme gives problems to the enzyme industries. A research was done to optimize the pullulanase activity from Bacillus Acidopullulyticus using experimental design. Two level Full Factorial design was applied in the screening process of four parameters for pullulanase activity and only three parameters were recognized as significant and effective factors for pullulanase activity. The three significant parameters were pH, enzyme and substrate concentration. Thus the three parameters were selected for further optimization studies using response surface methodology (RSM) in Design Expert version 7.1.6 software with Central Composite Design (CCD) mode. Thirty four sets of experiments with different parameter values were suggested. The predicted optimum values with R2 of 0.8729 for pH, enzyme concentration, substrate concentration and pullulanase activity were 6.60, 11.11 U/ml, 4.44% (w/v) and 3.17 U/ml respectively. One validation experiment was run using the optimized parameter and resulted 3.05 U/ml pullulanase activities as compared to the suggested values of 3.17 U/ml. As a conclusion, this research was successful to increase the pullulanase activity with a minimum numbers of experiments.
format	Undergraduates Project Papers
author	Norsalita, Abd Talib
author_facet	Norsalita, Abd Talib
author_sort	Norsalita, Abd Talib
title	Optimization of pullulanase activity through design of experiment (DOE)
title_short	Optimization of pullulanase activity through design of experiment (DOE)
title_full	Optimization of pullulanase activity through design of experiment (DOE)
title_fullStr	Optimization of pullulanase activity through design of experiment (DOE)
title_full_unstemmed	Optimization of pullulanase activity through design of experiment (DOE)
title_sort	optimization of pullulanase activity through design of experiment (doe)
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/3423/ http://umpir.ump.edu.my/id/eprint/3423/ http://umpir.ump.edu.my/id/eprint/3423/1/CD5948_NORSALITA_ABD_TALIB_X.pdf
first_indexed	2023-09-18T21:57:43Z
last_indexed	2023-09-18T21:57:43Z
_version_	1777414190706720768

Optimization of pullulanase activity through design of experiment (DOE)

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