Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate

In this paper, there were 2 main objectives. Those were to To determine the potential of delignification rice straw by using B.cereus and to study the PHB production from B.cereus by using rice straw as substrate. Delignification was crutial due to its chemical durability makes it indigestible to or...

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Main Author: Nor Syafarah, Zakariya
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/3429/
http://umpir.ump.edu.my/id/eprint/3429/
http://umpir.ump.edu.my/id/eprint/3429/1/CD5959_NOR_SYAFARAH_BITI_ZAKARIYA.pdf
id ump-3429
recordtype eprints
spelling ump-34292015-03-03T08:01:06Z http://umpir.ump.edu.my/id/eprint/3429/ Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate Nor Syafarah, Zakariya TP Chemical technology In this paper, there were 2 main objectives. Those were to To determine the potential of delignification rice straw by using B.cereus and to study the PHB production from B.cereus by using rice straw as substrate. Delignification was crutial due to its chemical durability makes it indigestible to organisms because of its bonding to cellulose and protein material. This lignin sheet acts as a barrier towards the outside elements (Carraher, 2008). The method used for delignification process was fermentation of B.cereus with 250 mL distilled water, 0.5 g yeast extract, 2.5 g peptone, 0.25 g MgSO4.7H 0 and 10 g of rice straw in a 500 mL shake flask. Then, the bacteria were fermented in the medium at 30 ºC and 250 rpm. For the analysis of delignification of rice straw by B.cereus, Klason’s method was utilized. From the result obtained, the highest lignin content was 4.56 % and the Control was 8.18 %. The percentage of the highest lignin degradation for the three samples was 98 %. The average lignin degradation was 78.67 %. After delignification, the bacteria could reacted on the cellulose content to synthesis PHB. The bacteria was fermented in 250 mL distilled water, 0.5 g yeast extract, 2.5 g peptone, 0.25 g MgSO24.7H0 and 1 g of delignified rice straw in a 500 mL shake flask. Then, the bacteria was fermented in the medium at 30 ºC and 250 rpm. The method of analysis of PHB yield was by using UV-vi spectrophotometer at 238nm. From the results obtained, the highest PHB yield is produced in Medium B; the medium consisted of MgSO24.7HO and yeast that yield 2.62 %. By these results, the objectives were achieved. 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3429/1/CD5959_NOR_SYAFARAH_BITI_ZAKARIYA.pdf Nor Syafarah, Zakariya (2010) Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:58533&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nor Syafarah, Zakariya
Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
description In this paper, there were 2 main objectives. Those were to To determine the potential of delignification rice straw by using B.cereus and to study the PHB production from B.cereus by using rice straw as substrate. Delignification was crutial due to its chemical durability makes it indigestible to organisms because of its bonding to cellulose and protein material. This lignin sheet acts as a barrier towards the outside elements (Carraher, 2008). The method used for delignification process was fermentation of B.cereus with 250 mL distilled water, 0.5 g yeast extract, 2.5 g peptone, 0.25 g MgSO4.7H 0 and 10 g of rice straw in a 500 mL shake flask. Then, the bacteria were fermented in the medium at 30 ºC and 250 rpm. For the analysis of delignification of rice straw by B.cereus, Klason’s method was utilized. From the result obtained, the highest lignin content was 4.56 % and the Control was 8.18 %. The percentage of the highest lignin degradation for the three samples was 98 %. The average lignin degradation was 78.67 %. After delignification, the bacteria could reacted on the cellulose content to synthesis PHB. The bacteria was fermented in 250 mL distilled water, 0.5 g yeast extract, 2.5 g peptone, 0.25 g MgSO24.7H0 and 1 g of delignified rice straw in a 500 mL shake flask. Then, the bacteria was fermented in the medium at 30 ºC and 250 rpm. The method of analysis of PHB yield was by using UV-vi spectrophotometer at 238nm. From the results obtained, the highest PHB yield is produced in Medium B; the medium consisted of MgSO24.7HO and yeast that yield 2.62 %. By these results, the objectives were achieved.
format Undergraduates Project Papers
author Nor Syafarah, Zakariya
author_facet Nor Syafarah, Zakariya
author_sort Nor Syafarah, Zakariya
title Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_short Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_full Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_fullStr Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_full_unstemmed Production of polyhydroxybutyrate (PHB) from bacillus cereus by using rice straw as substrate
title_sort production of polyhydroxybutyrate (phb) from bacillus cereus by using rice straw as substrate
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/3429/
http://umpir.ump.edu.my/id/eprint/3429/
http://umpir.ump.edu.my/id/eprint/3429/1/CD5959_NOR_SYAFARAH_BITI_ZAKARIYA.pdf
first_indexed 2023-09-18T21:57:43Z
last_indexed 2023-09-18T21:57:43Z
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