Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component

The uses of petroleum-based plastics are harmful because it cannot be degraded and is not environmental friendly. To overcome these problems, researchers have introduced a naturally occurring biopolymer which is polyhydroxybutyrate (PHB) as a substitute to the existing synthetic plastics. Polyhydrox...

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Main Author:	Kamaluddin Sarif, Sukardi
Format:	Undergraduates Project Papers
Language:	English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3599/ http://umpir.ump.edu.my/id/eprint/3599/1/CD6404_HAZWANI_ZULKIPLY.pdf

id	ump-3599
recordtype	eprints
spelling	ump-35992015-03-03T08:02:05Z http://umpir.ump.edu.my/id/eprint/3599/ Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component Kamaluddin Sarif, Sukardi TP Chemical technology The uses of petroleum-based plastics are harmful because it cannot be degraded and is not environmental friendly. To overcome these problems, researchers have introduced a naturally occurring biopolymer which is polyhydroxybutyrate (PHB) as a substitute to the existing synthetic plastics. Polyhydroxybutyrate is an intracellular product of bacteria fermentation at physiological stress condition. PHB is being studied because of its unique properties and because it is biodegradable. Production of bioplastic using PHB in large scale involved higher production costs compared to the production cost of existing petroleum-based plastics. Optimization of fermentation for PHB biopolymer was studied in shake flask by using Cupriavidus necator in oil palm trunk sap as the medium component. Three parameters were studied which were the fermentation temperature, agitation speed and percent volume of oil palm trunk sap. The optimum levels of parameters were determined by using mathematical model. Inocula were done in shake flask to cultivate the bacteria in growth medium solution. Fermentations were done in 500 mL shake flasks containing mineral salt, ultrapure water, inoculum and oil palm trunk sap. The flasks were incubated in incubator shaker for 24 hours at different levels of parameter values. The products were analysed for cell dry weight and PHB contents. Based on the experimental results obtained by using the Method of the Path of Steepest Ascent for the determination of the parameter values, the optimum condition of fermentation for PHB biopolymer was at temperature of 31.3°C, 123 rpm for agitation speed and 38.5 % for percent volume of oil palm trunk sap. The concentration of PHB obtained at the optimum condition was 0.825 g/L and the biomass content was 3.55 g/L. 2012-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3599/1/CD6404_HAZWANI_ZULKIPLY.pdf Kamaluddin Sarif, Sukardi (2012) Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component. Faculty of Chemical Engineering and Natural Resources, Universiti Malaysia Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Kamaluddin Sarif, Sukardi Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component
description	The uses of petroleum-based plastics are harmful because it cannot be degraded and is not environmental friendly. To overcome these problems, researchers have introduced a naturally occurring biopolymer which is polyhydroxybutyrate (PHB) as a substitute to the existing synthetic plastics. Polyhydroxybutyrate is an intracellular product of bacteria fermentation at physiological stress condition. PHB is being studied because of its unique properties and because it is biodegradable. Production of bioplastic using PHB in large scale involved higher production costs compared to the production cost of existing petroleum-based plastics. Optimization of fermentation for PHB biopolymer was studied in shake flask by using Cupriavidus necator in oil palm trunk sap as the medium component. Three parameters were studied which were the fermentation temperature, agitation speed and percent volume of oil palm trunk sap. The optimum levels of parameters were determined by using mathematical model. Inocula were done in shake flask to cultivate the bacteria in growth medium solution. Fermentations were done in 500 mL shake flasks containing mineral salt, ultrapure water, inoculum and oil palm trunk sap. The flasks were incubated in incubator shaker for 24 hours at different levels of parameter values. The products were analysed for cell dry weight and PHB contents. Based on the experimental results obtained by using the Method of the Path of Steepest Ascent for the determination of the parameter values, the optimum condition of fermentation for PHB biopolymer was at temperature of 31.3°C, 123 rpm for agitation speed and 38.5 % for percent volume of oil palm trunk sap. The concentration of PHB obtained at the optimum condition was 0.825 g/L and the biomass content was 3.55 g/L.
format	Undergraduates Project Papers
author	Kamaluddin Sarif, Sukardi
author_facet	Kamaluddin Sarif, Sukardi
author_sort	Kamaluddin Sarif, Sukardi
title	Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component
title_short	Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component
title_full	Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component
title_fullStr	Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component
title_full_unstemmed	Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component
title_sort	optimization of fermentation for phb biopolymer using oil palm trunk sap as medium component
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/3599/ http://umpir.ump.edu.my/id/eprint/3599/1/CD6404_HAZWANI_ZULKIPLY.pdf
first_indexed	2023-09-18T21:57:59Z
last_indexed	2023-09-18T21:57:59Z
_version_	1777414207381176320

Optimization of fermentation for phb biopolymer using oil palm trunk SAP as medium component

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