Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob

L-Asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an important enzyme which has anti-tumor properties. This paper describes the production and optimization of L-asparaginase by Bacillus sp. KK2S4 utilization of ground corn cob waste as substrate to reduce and manage the waste in our envi...

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Main Authors:	Makky, Essam A., Jee, Jian Ong, Karim, Md. Rezaul, Lee, Chin Mei
Format:	Article
Language:	English
Published:	African Journal of Biotechnology 2013
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/3780/ http://umpir.ump.edu.my/id/eprint/3780/ http://umpir.ump.edu.my/id/eprint/3780/1/Makky_fist.pdf

id	ump-3780
recordtype	eprints
spelling	ump-37802017-08-14T04:15:44Z http://umpir.ump.edu.my/id/eprint/3780/ Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob Makky, Essam A. Jee, Jian Ong Karim, Md. Rezaul Lee, Chin Mei TP Chemical technology L-Asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an important enzyme which has anti-tumor properties. This paper describes the production and optimization of L-asparaginase by Bacillus sp. KK2S4 utilization of ground corn cob waste as substrate to reduce and manage the waste in our environment. Thirty-nine (39) bacterial isolates were screened for their L-asparaginase productivity and protein content. Only Bacillus sp KK2S4 was identified before and selected as the most potent isolate. Modified M9 (MM9) minimal complete medium (glucose free) was used as production media for enzyme production. The highest enzyme production was detected at pH 5.0 in presence of lactose and NaNO3 as the best carbon and nitrogen sources with high activity increasing percentage of 1058.9, 377.3 and 566.7%, respectively. African Journal of Biotechnology 2013-05-08 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/3780/1/Makky_fist.pdf Makky, Essam A. and Jee, Jian Ong and Karim, Md. Rezaul and Lee, Chin Mei (2013) Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob. African Journal of Biotechnology, 12 (19). pp. 2654-2658. ISSN 1684-5315 http://www.academicjournals.org/ajb/abstracts/abs2013/8May/Makky%20et%20al.htm
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Makky, Essam A. Jee, Jian Ong Karim, Md. Rezaul Lee, Chin Mei Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob
description	L-Asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is an important enzyme which has anti-tumor properties. This paper describes the production and optimization of L-asparaginase by Bacillus sp. KK2S4 utilization of ground corn cob waste as substrate to reduce and manage the waste in our environment. Thirty-nine (39) bacterial isolates were screened for their L-asparaginase productivity and protein content. Only Bacillus sp KK2S4 was identified before and selected as the most potent isolate. Modified M9 (MM9) minimal complete medium (glucose free) was used as production media for enzyme production. The highest enzyme production was detected at pH 5.0 in presence of lactose and NaNO3 as the best carbon and nitrogen sources with high activity increasing percentage of 1058.9, 377.3 and 566.7%, respectively.
format	Article
author	Makky, Essam A. Jee, Jian Ong Karim, Md. Rezaul Lee, Chin Mei
author_facet	Makky, Essam A. Jee, Jian Ong Karim, Md. Rezaul Lee, Chin Mei
author_sort	Makky, Essam A.
title	Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob
title_short	Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob
title_full	Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob
title_fullStr	Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob
title_full_unstemmed	Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob
title_sort	production and optimization of l-asparaginase by bacillus sp. kk2s4 from corn cob
publisher	African Journal of Biotechnology
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/3780/ http://umpir.ump.edu.my/id/eprint/3780/ http://umpir.ump.edu.my/id/eprint/3780/1/Makky_fist.pdf
first_indexed	2023-09-18T21:58:17Z
last_indexed	2023-09-18T21:58:17Z
_version_	1777414226526076928

Production and Optimization of L-asparaginase by Bacillus sp. KK2S4 From Corn Cob

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