Screening of lignin degrader from soil

Since many years ago many researches have been done in order to find the latest, cheaper and more effective ways of getting out of lignin so that they can get to the cellulose fibers. Lignin is the main problems in the pulps and paper industries. The presence of lignin makes the newsprint turn yello...

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Main Author: Noor Zanida, Zamani
Format: Undergraduates Project Papers
Language:English
Published: 2008
Subjects:
Online Access:http://umpir.ump.edu.my/id/eprint/480/
http://umpir.ump.edu.my/id/eprint/480/
http://umpir.ump.edu.my/id/eprint/480/1/2717.pdf
id ump-480
recordtype eprints
spelling ump-4802015-03-03T06:03:40Z http://umpir.ump.edu.my/id/eprint/480/ Screening of lignin degrader from soil Noor Zanida, Zamani QK Botany Since many years ago many researches have been done in order to find the latest, cheaper and more effective ways of getting out of lignin so that they can get to the cellulose fibers. Lignin is the main problems in the pulps and paper industries. The presence of lignin makes the newsprint turn yellow. Besides that, the presence of lignin also contributed to problems such as acid gives off as the woods deteriorates. So that, in order to produce fine papers it is needed to removes the lignin. Biodegradation of lignin by microorganisms is the latest technology being found to replace the conventional method which using chemicals to remove lignin. Using microorganisms to removes lignin contributed in reducing cost and besides that, this method is really friendly to the environment. The main objectives of this study are to screen, to characterized microorganisms from soil that produce the lignin degrading enzymes and to analyze the enzyme activity. This process includes of two phases: screening process and the characterization process. Screening process was done using selective agar containing alkaline lignin. The microbe was characterized using simple staining, gram staining, and spore staining. The lignin degrading microbe found was tested; the result was a gram positive and, having endospore. The optimum pH was found to be in pH 7.0 while the optimum temperature discovered was 50°C with enzyme activity at 588.3 µmol/ml/min. 2008-05 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/480/1/2717.pdf Noor Zanida, Zamani (2008) Screening of lignin degrader from soil. Faculty of Chemical Engineering and Natural Resources, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:30880&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic QK Botany
spellingShingle QK Botany
Noor Zanida, Zamani
Screening of lignin degrader from soil
description Since many years ago many researches have been done in order to find the latest, cheaper and more effective ways of getting out of lignin so that they can get to the cellulose fibers. Lignin is the main problems in the pulps and paper industries. The presence of lignin makes the newsprint turn yellow. Besides that, the presence of lignin also contributed to problems such as acid gives off as the woods deteriorates. So that, in order to produce fine papers it is needed to removes the lignin. Biodegradation of lignin by microorganisms is the latest technology being found to replace the conventional method which using chemicals to remove lignin. Using microorganisms to removes lignin contributed in reducing cost and besides that, this method is really friendly to the environment. The main objectives of this study are to screen, to characterized microorganisms from soil that produce the lignin degrading enzymes and to analyze the enzyme activity. This process includes of two phases: screening process and the characterization process. Screening process was done using selective agar containing alkaline lignin. The microbe was characterized using simple staining, gram staining, and spore staining. The lignin degrading microbe found was tested; the result was a gram positive and, having endospore. The optimum pH was found to be in pH 7.0 while the optimum temperature discovered was 50°C with enzyme activity at 588.3 µmol/ml/min.
format Undergraduates Project Papers
author Noor Zanida, Zamani
author_facet Noor Zanida, Zamani
author_sort Noor Zanida, Zamani
title Screening of lignin degrader from soil
title_short Screening of lignin degrader from soil
title_full Screening of lignin degrader from soil
title_fullStr Screening of lignin degrader from soil
title_full_unstemmed Screening of lignin degrader from soil
title_sort screening of lignin degrader from soil
publishDate 2008
url http://umpir.ump.edu.my/id/eprint/480/
http://umpir.ump.edu.my/id/eprint/480/
http://umpir.ump.edu.my/id/eprint/480/1/2717.pdf
first_indexed 2023-09-18T21:52:44Z
last_indexed 2023-09-18T21:52:44Z
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