Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium

Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium

Bacterial cellulose is a type of biopolymer that produced by Acetobacter xylinum in high purity,high water holding capacity,good mechanical strength, elasticity and high crystallinity.In this research,pineapple residue was used as the carbon sources to replace the pure carbon sources as the substrat...

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Main Author: Muhammad Azlan, Nazeri
Format: Undergraduates Project Papers
Language:English
Published: 2012
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/5088/
http://umpir.ump.edu.my/id/eprint/5088/
http://umpir.ump.edu.my/id/eprint/5088/1/CD6496.pdf
id ump-5088
recordtype eprints
spelling ump-50882015-03-03T09:23:02Z http://umpir.ump.edu.my/id/eprint/5088/ Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium Muhammad Azlan, Nazeri TP Chemical technology Bacterial cellulose is a type of biopolymer that produced by Acetobacter xylinum in high purity,high water holding capacity,good mechanical strength, elasticity and high crystallinity.In this research,pineapple residue was used as the carbon sources to replace the pure carbon sources as the substrate for the synthesis of bacterial cellulose.The objective of this study was to investigate the effect of temperature,pH and concentration in the production of bacterial cellulose by Acetobacter xylinum.The important part in this research,including of preparation HS-Medium and agar plate as a medium for breeding the stock culture that was taken from Malaysia Agricultural Research and Development Institute (MARDI),Serdang,Selangor.Ideal condition in this research that investigated was varied from 40% to 100% for the concentration while the temperature was between 28°C to 32°C and pH were 4.5 to 8.5.Besides,this is study also aims to optimize the production of bacterial cellulose from pineapple residue using response surface methodology (RSM) based on the central composite design (CCD).Before RSM is used,the known value of the parameters was estimated based on one factor at that time (OFAT).The results obtained from the OFAT showed the optimum condition at pH 5.50,temperature 30°C and concentration of pineapple residue was 80 % where the amount of dry weight bacterial cellulose produced was 3.3948 g.According to the RSM result, the optimal set cultural conditions for bacterial cellulose were pH 5.15,temperature 30.51°C and concentration of pineapple residue was 83.32%.Bacterial cellulose production of 3.4368 g was achieved by using these optimal conditions. The existence of bacterial cellulose was proven by Fourier Transform Infrared (FT-IR) Spectroscopy analysis based on the appearance of absorbance peak for the C-C bonding, C-O bonding,C-OH bonding and C-O-C bonding.In addition,Scanning Electron Microscopy (SEM) was used to observe surface and cross section of the bacterial cellulose film.In short,the data presented in this paper showed that pineapple residue has a great potential as the carbon source in production of bacterial cellulose. 2012-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5088/1/CD6496.pdf Muhammad Azlan, Nazeri (2012) Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:67902&theme=UMP2
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Muhammad Azlan, Nazeri
Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium
description Bacterial cellulose is a type of biopolymer that produced by Acetobacter xylinum in high purity,high water holding capacity,good mechanical strength, elasticity and high crystallinity.In this research,pineapple residue was used as the carbon sources to replace the pure carbon sources as the substrate for the synthesis of bacterial cellulose.The objective of this study was to investigate the effect of temperature,pH and concentration in the production of bacterial cellulose by Acetobacter xylinum.The important part in this research,including of preparation HS-Medium and agar plate as a medium for breeding the stock culture that was taken from Malaysia Agricultural Research and Development Institute (MARDI),Serdang,Selangor.Ideal condition in this research that investigated was varied from 40% to 100% for the concentration while the temperature was between 28°C to 32°C and pH were 4.5 to 8.5.Besides,this is study also aims to optimize the production of bacterial cellulose from pineapple residue using response surface methodology (RSM) based on the central composite design (CCD).Before RSM is used,the known value of the parameters was estimated based on one factor at that time (OFAT).The results obtained from the OFAT showed the optimum condition at pH 5.50,temperature 30°C and concentration of pineapple residue was 80 % where the amount of dry weight bacterial cellulose produced was 3.3948 g.According to the RSM result, the optimal set cultural conditions for bacterial cellulose were pH 5.15,temperature 30.51°C and concentration of pineapple residue was 83.32%.Bacterial cellulose production of 3.4368 g was achieved by using these optimal conditions. The existence of bacterial cellulose was proven by Fourier Transform Infrared (FT-IR) Spectroscopy analysis based on the appearance of absorbance peak for the C-C bonding, C-O bonding,C-OH bonding and C-O-C bonding.In addition,Scanning Electron Microscopy (SEM) was used to observe surface and cross section of the bacterial cellulose film.In short,the data presented in this paper showed that pineapple residue has a great potential as the carbon source in production of bacterial cellulose.
format Undergraduates Project Papers
author Muhammad Azlan, Nazeri
author_facet Muhammad Azlan, Nazeri
author_sort Muhammad Azlan, Nazeri
title Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium
title_short Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium
title_full Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium
title_fullStr Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium
title_full_unstemmed Optimization of bacterial cellulose production by using response surface methodology(RSM)effect of PH,temperature and concentration of fermentation medium
title_sort optimization of bacterial cellulose production by using response surface methodology(rsm)effect of ph,temperature and concentration of fermentation medium
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/5088/
http://umpir.ump.edu.my/id/eprint/5088/
http://umpir.ump.edu.my/id/eprint/5088/1/CD6496.pdf
first_indexed 2023-09-18T22:00:14Z
last_indexed 2023-09-18T22:00:14Z
_version_ 1777414349286014976

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