Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii

Acetone,butanol and ethanol have widely been used in many industries.butanol and ethanol have been recognize as potential biofuel.While acetone is important as fuel tracer in field of combustion and use as suspension agent in pharmaceutical preparation.In 1990s,the increasing price of sugarcane have...

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Main Author:	Ma Umaira Suhaddha, Zainal Abidin
Format:	Undergraduates Project Papers
Language:	English
Published:	2012
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/5187/ http://umpir.ump.edu.my/id/eprint/5187/ http://umpir.ump.edu.my/id/eprint/5187/1/CD6379.pdf

id	ump-5187
recordtype	eprints
spelling	ump-51872015-03-03T09:23:40Z http://umpir.ump.edu.my/id/eprint/5187/ Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii Ma Umaira Suhaddha, Zainal Abidin TP Chemical technology Acetone,butanol and ethanol have widely been used in many industries.butanol and ethanol have been recognize as potential biofuel.While acetone is important as fuel tracer in field of combustion and use as suspension agent in pharmaceutical preparation.In 1990s,the increasing price of sugarcane have increased the production cost of ABE fermentation and the only factor that causing ABE fermentation could not survive was due to the cost of raw materials of substrate By using POME as substrate for fermentation process of acetone,butanol and ethanol(ABE), production is at advantage due to its lower cost and readily available.This study is to determine the effect of concentration of substrate,modified glucose concentration on substrate and initial pH of substrate towards enhancing solvent production from palm oil mill effluent(POME) by Clostridium beijerinckii.The experiment has been carried out in a batch culture system.The study of substrate pH effect ranged from 4.7, 5.2, 5.7 to 6.2.Meanwhile, for study of substrate concentration ranged from 60%,70%,80%,90% to 100%. The study of modified substrate concentration effect were ranging from 20g/L,30g/L,40g/L,50g/L to 60g/L.The fermentation of POME which produced acetone,butanol and ethanol,was analysed using gas chromatography flame ionization detector(GC-FID)and isobutanol was used as the internal standard.The experiment was using one factor at one time (OFAT) method to determine the best range values.From the parameters stated,sedimented POME at 70% concentration with initial pH increased to 5.7 is most suitable for solvent production 70% with 1.025g/L solvent produced.While 50g/L is the optimal concentration of modified glucose that enhanced the solvent production with supported by operating temperature at 37°c and 150rpm which 1.241g/L solvent produced.Apart from that,growth cell at pH5.7 maximize the solvent production where 0.967g/L solvent produced.In addition,DNS test for glucose consumption of all parameters studies can be conclude that higher glucose consumption resulted in higher amount of total solvent produced.The results obtained from these experiments showed that POME could be a viable media for ABE fermentation. 2012-01 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5187/1/CD6379.pdf Ma Umaira Suhaddha, Zainal Abidin (2012) Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:66767&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Ma Umaira Suhaddha, Zainal Abidin Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii
description	Acetone,butanol and ethanol have widely been used in many industries.butanol and ethanol have been recognize as potential biofuel.While acetone is important as fuel tracer in field of combustion and use as suspension agent in pharmaceutical preparation.In 1990s,the increasing price of sugarcane have increased the production cost of ABE fermentation and the only factor that causing ABE fermentation could not survive was due to the cost of raw materials of substrate By using POME as substrate for fermentation process of acetone,butanol and ethanol(ABE), production is at advantage due to its lower cost and readily available.This study is to determine the effect of concentration of substrate,modified glucose concentration on substrate and initial pH of substrate towards enhancing solvent production from palm oil mill effluent(POME) by Clostridium beijerinckii.The experiment has been carried out in a batch culture system.The study of substrate pH effect ranged from 4.7, 5.2, 5.7 to 6.2.Meanwhile, for study of substrate concentration ranged from 60%,70%,80%,90% to 100%. The study of modified substrate concentration effect were ranging from 20g/L,30g/L,40g/L,50g/L to 60g/L.The fermentation of POME which produced acetone,butanol and ethanol,was analysed using gas chromatography flame ionization detector(GC-FID)and isobutanol was used as the internal standard.The experiment was using one factor at one time (OFAT) method to determine the best range values.From the parameters stated,sedimented POME at 70% concentration with initial pH increased to 5.7 is most suitable for solvent production 70% with 1.025g/L solvent produced.While 50g/L is the optimal concentration of modified glucose that enhanced the solvent production with supported by operating temperature at 37°c and 150rpm which 1.241g/L solvent produced.Apart from that,growth cell at pH5.7 maximize the solvent production where 0.967g/L solvent produced.In addition,DNS test for glucose consumption of all parameters studies can be conclude that higher glucose consumption resulted in higher amount of total solvent produced.The results obtained from these experiments showed that POME could be a viable media for ABE fermentation.
format	Undergraduates Project Papers
author	Ma Umaira Suhaddha, Zainal Abidin
author_facet	Ma Umaira Suhaddha, Zainal Abidin
author_sort	Ma Umaira Suhaddha, Zainal Abidin
title	Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii
title_short	Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii
title_full	Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii
title_fullStr	Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii
title_full_unstemmed	Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii
title_sort	solvent production from palm oil mill effluent (pome) by clostridium beijerinckii
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/5187/ http://umpir.ump.edu.my/id/eprint/5187/ http://umpir.ump.edu.my/id/eprint/5187/1/CD6379.pdf
first_indexed	2023-09-18T22:00:25Z
last_indexed	2023-09-18T22:00:25Z
_version_	1777414361283821568

Solvent production from palm oil mill effluent (POME) by clostridium beijerinckii

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