Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter

Plastics have been an integral part of our life. However, disposal of these non-biodegradable (petrochemical derived) plastics poses a threat to our environment. In an effort to overcome these shortcomings, biochemical researchers and engineers have long been seeking to develop biodegradable plastic...

Full description

Bibliographic Details
Main Author:	Goh, Mei Fong
Format:	Undergraduates Project Papers
Language:	English
Published:	2008
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/519/ http://umpir.ump.edu.my/id/eprint/519/1/2709.pdf

id	ump-519
recordtype	eprints
spelling	ump-5192015-03-03T06:03:54Z http://umpir.ump.edu.my/id/eprint/519/ Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter Goh, Mei Fong TA Engineering (General). Civil engineering (General) Plastics have been an integral part of our life. However, disposal of these non-biodegradable (petrochemical derived) plastics poses a threat to our environment. In an effort to overcome these shortcomings, biochemical researchers and engineers have long been seeking to develop biodegradable plastics that are made from renewable resources. Polyhydroxybutyrates (PHB) are polymers that accumulate as carbon and energy in Cupriavidus necator and provide an alternative to petrochemical plastic because of their biodegradability properties. However, major problems in commercializing PHB is the high production cost due to expensive carbon substrates and tedious production procedures using pure cultures. Therefore, the applications of mixed cultures and cheap carbon sources have been explored. In this study, the biopolymer fermentor has to scale up from shake flask to 10L of stirred tank fermentor. This is to increase the mass production of PHB that produced by Cupriavidus necator. The biopolymer fermentor is scaled up is by fixing the “kLa”. kLa value were derived by fitting the mass transfer equation to the data of dissolved oxygen tension (DOT) versus time on computer using Matlab method with two unknowns, namely kLa and the electrode mass transfer coefficient (kLa) of oxygen. The stirred speed (rpm) and the air flow rate (A) in the 10L fermentor that produced the value of kLa found in the optimized conditions in shake flask was approximated by trail an error. Overall, scale up by using the method of constant volumetric oxygen transfer coefficient (kLa) in the 10L stirred tank fermentor will produce the same PHB production as in the shake flask. 2008-04-28 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/519/1/2709.pdf Goh, Mei Fong (2008) Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter. Faculty of Chemical Engineering and Natural Resources, Universiti Malaysia Pahang.
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Goh, Mei Fong Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter
description	Plastics have been an integral part of our life. However, disposal of these non-biodegradable (petrochemical derived) plastics poses a threat to our environment. In an effort to overcome these shortcomings, biochemical researchers and engineers have long been seeking to develop biodegradable plastics that are made from renewable resources. Polyhydroxybutyrates (PHB) are polymers that accumulate as carbon and energy in Cupriavidus necator and provide an alternative to petrochemical plastic because of their biodegradability properties. However, major problems in commercializing PHB is the high production cost due to expensive carbon substrates and tedious production procedures using pure cultures. Therefore, the applications of mixed cultures and cheap carbon sources have been explored. In this study, the biopolymer fermentor has to scale up from shake flask to 10L of stirred tank fermentor. This is to increase the mass production of PHB that produced by Cupriavidus necator. The biopolymer fermentor is scaled up is by fixing the “kLa”. kLa value were derived by fitting the mass transfer equation to the data of dissolved oxygen tension (DOT) versus time on computer using Matlab method with two unknowns, namely kLa and the electrode mass transfer coefficient (kLa) of oxygen. The stirred speed (rpm) and the air flow rate (A) in the 10L fermentor that produced the value of kLa found in the optimized conditions in shake flask was approximated by trail an error. Overall, scale up by using the method of constant volumetric oxygen transfer coefficient (kLa) in the 10L stirred tank fermentor will produce the same PHB production as in the shake flask.
format	Undergraduates Project Papers
author	Goh, Mei Fong
author_facet	Goh, Mei Fong
author_sort	Goh, Mei Fong
title	Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter
title_short	Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter
title_full	Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter
title_fullStr	Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter
title_full_unstemmed	Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter
title_sort	scale up the biopolymer (phb) fermentation from shake flasks to 10l stirred tank fermenter
publishDate	2008
url	http://umpir.ump.edu.my/id/eprint/519/ http://umpir.ump.edu.my/id/eprint/519/1/2709.pdf
first_indexed	2023-09-18T21:52:49Z
last_indexed	2023-09-18T21:52:49Z
_version_	1777413882436911104

Scale up the biopolymer (PHB) fermentation from shake flasks to 10l stirred tank fermenter

Similar Items