Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique

The aim for this research is to produces protein separation tool by a new technique to separate protein molecule from protein solution.The technique used is the molecularly imprinted polymer,MIP.In this technique the target molecule that we want to separate will be the template and it mixed with sel...

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Main Author:	Mohd.Raflie , Maslih
Format:	Undergraduates Project Papers
Language:	English
Published:	2010
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/5358/ http://umpir.ump.edu.my/id/eprint/5358/ http://umpir.ump.edu.my/id/eprint/5358/1/CD5874.pdf

id	ump-5358
recordtype	eprints
spelling	ump-53582015-03-03T09:24:25Z http://umpir.ump.edu.my/id/eprint/5358/ Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique Mohd.Raflie , Maslih TP Chemical technology The aim for this research is to produces protein separation tool by a new technique to separate protein molecule from protein solution.The technique used is the molecularly imprinted polymer,MIP.In this technique the target molecule that we want to separate will be the template and it mixed with selected monomers.Through polymerization process the template molecule will trapped between the polymer matrix that become solid and stable in term of heat and chemical. To make it has high selectivity on protein molecule the solid polymer is pounded to fine particles and through hydrolysis process the template is extracted and will leave complementary sequences that have exact configuration like the template shape.And further,two type of study were carried out, the first was analysis of particles structure in characterization of particles by using FT-IR spectrum and the second was study on the performances of this technique by conducting three experiments.The first experiment was the adsorption of BSA protein in MIP or synthesized polymer particles,the second experiment was the effect of protein concentration on separation and third experiment was to observe the effect of particles mass on separation. Polymerization and hydrolysis was successfully occur and it shown by the FT-IR spectrum.Experiment show that adsorption of protein was high for MIP particle that was 52.5 % while adsorption for NIP particle was 33.0 %.For the effect of concentration and mass of particle, it shows that the availability particle is the main factor that influence the amount of substrate separated 2010-04 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5358/1/CD5874.pdf Mohd.Raflie , Maslih (2010) Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique. Faculty of Chemical & Natural Resource Engineering, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:58861&theme=UMP2
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Mohd.Raflie , Maslih Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique
description	The aim for this research is to produces protein separation tool by a new technique to separate protein molecule from protein solution.The technique used is the molecularly imprinted polymer,MIP.In this technique the target molecule that we want to separate will be the template and it mixed with selected monomers.Through polymerization process the template molecule will trapped between the polymer matrix that become solid and stable in term of heat and chemical. To make it has high selectivity on protein molecule the solid polymer is pounded to fine particles and through hydrolysis process the template is extracted and will leave complementary sequences that have exact configuration like the template shape.And further,two type of study were carried out, the first was analysis of particles structure in characterization of particles by using FT-IR spectrum and the second was study on the performances of this technique by conducting three experiments.The first experiment was the adsorption of BSA protein in MIP or synthesized polymer particles,the second experiment was the effect of protein concentration on separation and third experiment was to observe the effect of particles mass on separation. Polymerization and hydrolysis was successfully occur and it shown by the FT-IR spectrum.Experiment show that adsorption of protein was high for MIP particle that was 52.5 % while adsorption for NIP particle was 33.0 %.For the effect of concentration and mass of particle, it shows that the availability particle is the main factor that influence the amount of substrate separated
format	Undergraduates Project Papers
author	Mohd.Raflie , Maslih
author_facet	Mohd.Raflie , Maslih
author_sort	Mohd.Raflie , Maslih
title	Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique
title_short	Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique
title_full	Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique
title_fullStr	Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique
title_full_unstemmed	Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique
title_sort	selective recognition of bovine serum albumin (bsa) using molecular imprinting polymer technique
publishDate	2010
url	http://umpir.ump.edu.my/id/eprint/5358/ http://umpir.ump.edu.my/id/eprint/5358/ http://umpir.ump.edu.my/id/eprint/5358/1/CD5874.pdf
first_indexed	2023-09-18T22:00:40Z
last_indexed	2023-09-18T22:00:40Z
_version_	1777414376591982592

Selective recognition of bovine serum albumin (BSA) using molecular imprinting polymer technique

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