Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipita...

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Main Authors:	Siti Hatijah, Mortan, Wan Ruhayu, Wan Ramli
Format:	Article
Language:	English
Published:	International Academy Publishing (IAP) 2013
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/5549/ http://umpir.ump.edu.my/id/eprint/5549/ http://umpir.ump.edu.my/id/eprint/5549/ http://umpir.ump.edu.my/id/eprint/5549/1/267-S30006.pdf

id	ump-5549
recordtype	eprints
spelling	ump-55492018-05-15T06:45:48Z http://umpir.ump.edu.my/id/eprint/5549/ Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus Siti Hatijah, Mortan Wan Ruhayu, Wan Ramli TP Chemical technology Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipitation of uric acid can leading to gout symptom. The main purpose of this research is to optimize the culture condition for maximum uricase production by Aspergillus flavus. The parameters studied were pH (ranged pH4 to pH8),the sucrose concentration (10g/l to 50 g/l) and the agitation rate(100 to 300 rpm). The Aspergillus flavus were inoculated in yeast extract with sucrose and incubated for 24 hours at 200 rpm and 30oC. The maximum enzyme activity obtained from the experiment is 0.03974 U/ml at pH 6, 200 rpm and 30 g/l sucrose concentration. International Academy Publishing (IAP) 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/5549/1/267-S30006.pdf Siti Hatijah, Mortan and Wan Ruhayu, Wan Ramli (2013) Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus. International Journal of Bioscience, Biochemistry and Bioinformatics, 3 (5). pp. 513-515. ISSN 2010-3638 http://dx.doi.org/10.7763/IJBBB.2013.V3.267 DOI: 10.7763/IJBBB.2013.V3.267
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Siti Hatijah, Mortan Wan Ruhayu, Wan Ramli Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
description	Urate oxidase or uricase is an enzyme that catalyses the oxidation of uric acid to allantoin and plays important rule in purine metabolism. The first important application discovered for uricase was in clinical biochemistry as diagnosis reagent for measurement of uric acid in blood. The precipitation of uric acid can leading to gout symptom. The main purpose of this research is to optimize the culture condition for maximum uricase production by Aspergillus flavus. The parameters studied were pH (ranged pH4 to pH8),the sucrose concentration (10g/l to 50 g/l) and the agitation rate(100 to 300 rpm). The Aspergillus flavus were inoculated in yeast extract with sucrose and incubated for 24 hours at 200 rpm and 30oC. The maximum enzyme activity obtained from the experiment is 0.03974 U/ml at pH 6, 200 rpm and 30 g/l sucrose concentration.
format	Article
author	Siti Hatijah, Mortan Wan Ruhayu, Wan Ramli
author_facet	Siti Hatijah, Mortan Wan Ruhayu, Wan Ramli
author_sort	Siti Hatijah, Mortan
title	Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_short	Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_full	Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_fullStr	Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_full_unstemmed	Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus
title_sort	preliminary study on factors that enhanced the production of uricase by aspergillus flavus
publisher	International Academy Publishing (IAP)
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/5549/ http://umpir.ump.edu.my/id/eprint/5549/ http://umpir.ump.edu.my/id/eprint/5549/ http://umpir.ump.edu.my/id/eprint/5549/1/267-S30006.pdf
first_indexed	2023-09-18T22:00:53Z
last_indexed	2023-09-18T22:00:53Z
_version_	1777414390057795584

Preliminary Study on Factors that Enhanced the Production of Uricase by Aspergillus Flavus

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