Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast

Bio-Ethanol is a renewable; eco-friendly energy source can be produced from bio-mass (hemicelluloses). Pteris (fern) is grown very fast and has not major economic importance but it is a reliable source of hemicelluloses can be converted to bio-Ethanol. In the hydrolysis of hemicelluloses the con...

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Main Authors: Khan, Maksudur R., Saha, Pradip, Fakhrul Alam, Md., Baishnab, Ajit Chandra, Islam, M. A.
Format: Article
Language:English
Published: Science and Education Publishing 2014
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Online Access:http://umpir.ump.edu.my/id/eprint/6129/
http://umpir.ump.edu.my/id/eprint/6129/
http://umpir.ump.edu.my/id/eprint/6129/
http://umpir.ump.edu.my/id/eprint/6129/1/Production_of_Bio-fuel_%28Bio-Ethanol%29_from_Biomass_%28Pteris%29_by_Fermentation_Process_with_Yeast.pdf
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spelling ump-61292018-01-16T01:20:23Z http://umpir.ump.edu.my/id/eprint/6129/ Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast Khan, Maksudur R. Saha, Pradip Fakhrul Alam, Md. Baishnab, Ajit Chandra Islam, M. A. TP Chemical technology Bio-Ethanol is a renewable; eco-friendly energy source can be produced from bio-mass (hemicelluloses). Pteris (fern) is grown very fast and has not major economic importance but it is a reliable source of hemicelluloses can be converted to bio-Ethanol. In the hydrolysis of hemicelluloses the concentration of glucose and the reaction rate was observed with respect to the different parameters, like pH, temperature, substrate diameter, substrate loading. In this study we found optimal parameters, NH4OH treatment as the best treatment among H2SO4, NH4OH, and NaOH treatment. In addition, pH 7, temperature 35°C, substrate diameter 45μm-63μm, and substrate loading 0.25gm in 100ml working volume was found as optimum operation condition for hydrolysis reaction, which was carried out by pseudomonas sp., isolated from cow dung. In this experiment sugar concentration was measured with UV spectrophotometer using DNS reagent finding the equilibrium time is 72hours for hydrolysis process and the maximum sugar concentration of 1.7625mg/l was achieved. Subsequently we study the fermentation process using yeast to produce Bio-Ethanol from reducing sugar solution obtained from the hydrolysis process on optimum reaction condition and yield the Ethanol concentration 0.333 mg/L, measured with UV spectrophotometer. Furthermore, it was also observed that the activity of yeast was sustain, in the reaction condition (pH = 7, temperature = 25°C), only for 50 hours. In sum, it could be conclude that 0.1332 mg of ethanol can be produce from 1gm of pteris where the conversion of reducing sugar to ethanol is 20% approximately in the optimum reaction condition. Science and Education Publishing 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6129/1/Production_of_Bio-fuel_%28Bio-Ethanol%29_from_Biomass_%28Pteris%29_by_Fermentation_Process_with_Yeast.pdf Khan, Maksudur R. and Saha, Pradip and Fakhrul Alam, Md. and Baishnab, Ajit Chandra and Islam, M. A. (2014) Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast. Sustainable Energy , 2 (1). pp. 25-28. ISSN 2164-9219 (print); 2164-9065 (online) http://pubs.sciepub.com/rse/2/1/5/ DOI: 10.12691/rse-2-1-5
repository_type Digital Repository
institution_category Local University
institution Universiti Malaysia Pahang
building UMP Institutional Repository
collection Online Access
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Khan, Maksudur R.
Saha, Pradip
Fakhrul Alam, Md.
Baishnab, Ajit Chandra
Islam, M. A.
Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast
description Bio-Ethanol is a renewable; eco-friendly energy source can be produced from bio-mass (hemicelluloses). Pteris (fern) is grown very fast and has not major economic importance but it is a reliable source of hemicelluloses can be converted to bio-Ethanol. In the hydrolysis of hemicelluloses the concentration of glucose and the reaction rate was observed with respect to the different parameters, like pH, temperature, substrate diameter, substrate loading. In this study we found optimal parameters, NH4OH treatment as the best treatment among H2SO4, NH4OH, and NaOH treatment. In addition, pH 7, temperature 35°C, substrate diameter 45μm-63μm, and substrate loading 0.25gm in 100ml working volume was found as optimum operation condition for hydrolysis reaction, which was carried out by pseudomonas sp., isolated from cow dung. In this experiment sugar concentration was measured with UV spectrophotometer using DNS reagent finding the equilibrium time is 72hours for hydrolysis process and the maximum sugar concentration of 1.7625mg/l was achieved. Subsequently we study the fermentation process using yeast to produce Bio-Ethanol from reducing sugar solution obtained from the hydrolysis process on optimum reaction condition and yield the Ethanol concentration 0.333 mg/L, measured with UV spectrophotometer. Furthermore, it was also observed that the activity of yeast was sustain, in the reaction condition (pH = 7, temperature = 25°C), only for 50 hours. In sum, it could be conclude that 0.1332 mg of ethanol can be produce from 1gm of pteris where the conversion of reducing sugar to ethanol is 20% approximately in the optimum reaction condition.
format Article
author Khan, Maksudur R.
Saha, Pradip
Fakhrul Alam, Md.
Baishnab, Ajit Chandra
Islam, M. A.
author_facet Khan, Maksudur R.
Saha, Pradip
Fakhrul Alam, Md.
Baishnab, Ajit Chandra
Islam, M. A.
author_sort Khan, Maksudur R.
title Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast
title_short Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast
title_full Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast
title_fullStr Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast
title_full_unstemmed Production of Bio-fuel (Bio-Ethanol) from Biomass (Pteris) by Fermentation Process with Yeast
title_sort production of bio-fuel (bio-ethanol) from biomass (pteris) by fermentation process with yeast
publisher Science and Education Publishing
publishDate 2014
url http://umpir.ump.edu.my/id/eprint/6129/
http://umpir.ump.edu.my/id/eprint/6129/
http://umpir.ump.edu.my/id/eprint/6129/
http://umpir.ump.edu.my/id/eprint/6129/1/Production_of_Bio-fuel_%28Bio-Ethanol%29_from_Biomass_%28Pteris%29_by_Fermentation_Process_with_Yeast.pdf
first_indexed 2023-09-18T22:01:38Z
last_indexed 2023-09-18T22:01:38Z
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