Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls

The aim of this study was to evaluate the effect of two semen collection methods (electro-ejaculation [EE] and transrectal massage [RM]) on in vitro sperm functionality and protein composition of seminal plasma in Bali bulls. Ten untrained Bali bulls were selected for semen collection by EE and RM....

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Main Authors:	Sarsaifi, Kazhal, Jaya Vejayan, Palliah, Abd Wahid, Haron, Rosnina, Yusoff, Homayoun, Hani, Mina, Rasoli, Mohamed Ariff, Omar, Abas Mazni, Othman
Format:	Article
Language:	English
Published:	Elsevier Ltd 2015
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/6196/ http://umpir.ump.edu.my/id/eprint/6196/ http://umpir.ump.edu.my/id/eprint/6196/ http://umpir.ump.edu.my/id/eprint/6196/1/Protein%20profile%20and%20functionality%20of%20spermatozoa%20from%20two%20semen%20collection%20methods%20in%20Bali%20bulls.pdf

id	ump-6196
recordtype	eprints
spelling	ump-61962018-02-21T07:00:21Z http://umpir.ump.edu.my/id/eprint/6196/ Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls Sarsaifi, Kazhal Jaya Vejayan, Palliah Abd Wahid, Haron Rosnina, Yusoff Homayoun, Hani Mina, Rasoli Mohamed Ariff, Omar Abas Mazni, Othman Q Science (General) The aim of this study was to evaluate the effect of two semen collection methods (electro-ejaculation [EE] and transrectal massage [RM]) on in vitro sperm functionality and protein composition of seminal plasma in Bali bulls. Ten untrained Bali bulls were selected for semen collection by EE and RM. Parameters analysed were acrosome and plasma membrane integrity, sperm motility (by CASA), normal morphology, functionality (sperm penetration assay), acrosome reaction, total protein content and protein profiles (by 2D-PAGE). Bulls collected by RM had a higher (p<0.05) percentage of spermatozoa with intact acrosome and plasma membrane, functionality and individual motility, and a lower proportion of seminal plasma, total protein content and lower ratio of low molecular weight proteins than those collected by EE. Analysis of 2D-PAGE gel detected about 116 spots in the range of 10–250 kDa and isoelectric points (pI) ranging from 3 to 10. Approximately 52% of seminal plasma protein spots were represented by four major protein fractions with molecular weights around 37–45 kDa (15.66%), 25–30 kDa (12.46%), 14–16 kDa (11.73%) and 12–15 kDa (11.52%). Ten of the seminal plasma proteins identified by mass spectrometry belonged to major bovine seminal plasma proteins. A very significant finding in this study was related to the two proteins identified, PGK and PLA2, with MW of approximately 37–40 and 50–55 kDa and pI of 8.5–8.8 and 5.2–6.0, respectively. These two protein spots can only be detected in the seminal plasma of ejaculates obtained through RM. In conclusion, semen quality as examined by in vitro sperm functionality was found to be better in RM than EE samples after treatment with heparin and calcium ionophore A-23187. In addition some low molecular weight proteins were up-regulated in the seminal plasma obtained from the EE method. Elsevier Ltd 2015 Article PeerReviewed application/pdf en cc_by_nc_nd http://umpir.ump.edu.my/id/eprint/6196/1/Protein%20profile%20and%20functionality%20of%20spermatozoa%20from%20two%20semen%20collection%20methods%20in%20Bali%20bulls.pdf Sarsaifi, Kazhal and Jaya Vejayan, Palliah and Abd Wahid, Haron and Rosnina, Yusoff and Homayoun, Hani and Mina, Rasoli and Mohamed Ariff, Omar and Abas Mazni, Othman (2015) Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls. Livestock Science, 172. pp. 96-105. ISSN 1871-1413 http://dx.doi.org/10.1016/j.livsci.2014.12.004 doi: 10.1016/j.livsci.2014.12.004
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Sarsaifi, Kazhal Jaya Vejayan, Palliah Abd Wahid, Haron Rosnina, Yusoff Homayoun, Hani Mina, Rasoli Mohamed Ariff, Omar Abas Mazni, Othman Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls
description	The aim of this study was to evaluate the effect of two semen collection methods (electro-ejaculation [EE] and transrectal massage [RM]) on in vitro sperm functionality and protein composition of seminal plasma in Bali bulls. Ten untrained Bali bulls were selected for semen collection by EE and RM. Parameters analysed were acrosome and plasma membrane integrity, sperm motility (by CASA), normal morphology, functionality (sperm penetration assay), acrosome reaction, total protein content and protein profiles (by 2D-PAGE). Bulls collected by RM had a higher (p<0.05) percentage of spermatozoa with intact acrosome and plasma membrane, functionality and individual motility, and a lower proportion of seminal plasma, total protein content and lower ratio of low molecular weight proteins than those collected by EE. Analysis of 2D-PAGE gel detected about 116 spots in the range of 10–250 kDa and isoelectric points (pI) ranging from 3 to 10. Approximately 52% of seminal plasma protein spots were represented by four major protein fractions with molecular weights around 37–45 kDa (15.66%), 25–30 kDa (12.46%), 14–16 kDa (11.73%) and 12–15 kDa (11.52%). Ten of the seminal plasma proteins identified by mass spectrometry belonged to major bovine seminal plasma proteins. A very significant finding in this study was related to the two proteins identified, PGK and PLA2, with MW of approximately 37–40 and 50–55 kDa and pI of 8.5–8.8 and 5.2–6.0, respectively. These two protein spots can only be detected in the seminal plasma of ejaculates obtained through RM. In conclusion, semen quality as examined by in vitro sperm functionality was found to be better in RM than EE samples after treatment with heparin and calcium ionophore A-23187. In addition some low molecular weight proteins were up-regulated in the seminal plasma obtained from the EE method.
format	Article
author	Sarsaifi, Kazhal Jaya Vejayan, Palliah Abd Wahid, Haron Rosnina, Yusoff Homayoun, Hani Mina, Rasoli Mohamed Ariff, Omar Abas Mazni, Othman
author_facet	Sarsaifi, Kazhal Jaya Vejayan, Palliah Abd Wahid, Haron Rosnina, Yusoff Homayoun, Hani Mina, Rasoli Mohamed Ariff, Omar Abas Mazni, Othman
author_sort	Sarsaifi, Kazhal
title	Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls
title_short	Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls
title_full	Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls
title_fullStr	Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls
title_full_unstemmed	Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls
title_sort	protein profile and functionality of spermatozoa from two semen collection methods in bali bulls
publisher	Elsevier Ltd
publishDate	2015
url	http://umpir.ump.edu.my/id/eprint/6196/ http://umpir.ump.edu.my/id/eprint/6196/ http://umpir.ump.edu.my/id/eprint/6196/ http://umpir.ump.edu.my/id/eprint/6196/1/Protein%20profile%20and%20functionality%20of%20spermatozoa%20from%20two%20semen%20collection%20methods%20in%20Bali%20bulls.pdf
first_indexed	2023-09-18T22:01:46Z
last_indexed	2023-09-18T22:01:46Z
_version_	1777414446025539584

Protein Profile and Functionality of Spermatozoa from Two Semen Collection Methods in Bali Bulls

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