Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor

A novel polyethylene glycol (PEG) gel was fabricated and used as a carrier to immobilize Clostridium sp. LS2 for continuous hydrogen production in an upflow anaerobic sludge blanket (UASB) reactor. Palm oil mill effluent (POME) was used as the substrate carbon source. The optimal amount of PEG-immob...

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Main Authors:	Singh, Lakhveer, Muhammad Faisal, Siddiqui, Anwar, Ahmad, Mohd Hasbi, Ab. Rahim, Mimi Sakinah, A. M., Zularisam, A. W.
Format:	Article
Language:	English
Published:	Elsevier Ltd 2013
Subjects:	Q Science (General) TP Chemical technology TA Engineering (General). Civil engineering (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/6243/ http://umpir.ump.edu.my/id/eprint/6243/ http://umpir.ump.edu.my/id/eprint/6243/ http://umpir.ump.edu.my/id/eprint/6243/1/fist-lakhveer-Application_of_polyethylene_abs_only.pdf

id	ump-6243
recordtype	eprints
spelling	ump-62432018-11-22T06:55:23Z http://umpir.ump.edu.my/id/eprint/6243/ Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor Singh, Lakhveer Muhammad Faisal, Siddiqui Anwar, Ahmad Mohd Hasbi, Ab. Rahim Mimi Sakinah, A. M. Zularisam, A. W. Q Science (General) TP Chemical technology TA Engineering (General). Civil engineering (General) A novel polyethylene glycol (PEG) gel was fabricated and used as a carrier to immobilize Clostridium sp. LS2 for continuous hydrogen production in an upflow anaerobic sludge blanket (UASB) reactor. Palm oil mill effluent (POME) was used as the substrate carbon source. The optimal amount of PEG-immobilized cells for anaerobic hydrogen production was 12% (w/v) in the UASB reactor. The UASB reactor containing immobilized cells was operated at varying hydraulic retention times (HRT) that ranged from 24 to 6 h at 3.3 g chemical oxygen demand (COD)/L/h organic loading rate (OLR), or at OLRs that ranged from 1.6 to 6.6 at 12 h HRT. The best volumetric hydrogen production rate of 336 mL H2/L/h (or 15.0 mmol/L/h) with a hydrogen yield of 0.35 L H2/g CODremoved was obtained at a HRT of 12 h and an OLR of 5.0 g COD/L/h. The average hydrogen content of biogas and COD reduction were 52% and 62%, respectively. The major soluble metabolites during hydrogen fermentation were butyric acid followed by acetic acid. It is concluded that the PEG-immobilized cell system developed in this work has great potential for continuous hydrogen production from real wastewater (POME) using the UASB reactor. Elsevier Ltd 2013 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6243/1/fist-lakhveer-Application_of_polyethylene_abs_only.pdf Singh, Lakhveer and Muhammad Faisal, Siddiqui and Anwar, Ahmad and Mohd Hasbi, Ab. Rahim and Mimi Sakinah, A. M. and Zularisam, A. W. (2013) Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor. Biochemical Engineering Journal, 70. pp. 158-165. ISSN 1369-703X http://dx.doi.org/10.1016/j.bej.2012.10.010 DOI:10.1016/j.bej.2012.10.010
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	Q Science (General) TP Chemical technology TA Engineering (General). Civil engineering (General)
spellingShingle	Q Science (General) TP Chemical technology TA Engineering (General). Civil engineering (General) Singh, Lakhveer Muhammad Faisal, Siddiqui Anwar, Ahmad Mohd Hasbi, Ab. Rahim Mimi Sakinah, A. M. Zularisam, A. W. Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor
description	A novel polyethylene glycol (PEG) gel was fabricated and used as a carrier to immobilize Clostridium sp. LS2 for continuous hydrogen production in an upflow anaerobic sludge blanket (UASB) reactor. Palm oil mill effluent (POME) was used as the substrate carbon source. The optimal amount of PEG-immobilized cells for anaerobic hydrogen production was 12% (w/v) in the UASB reactor. The UASB reactor containing immobilized cells was operated at varying hydraulic retention times (HRT) that ranged from 24 to 6 h at 3.3 g chemical oxygen demand (COD)/L/h organic loading rate (OLR), or at OLRs that ranged from 1.6 to 6.6 at 12 h HRT. The best volumetric hydrogen production rate of 336 mL H2/L/h (or 15.0 mmol/L/h) with a hydrogen yield of 0.35 L H2/g CODremoved was obtained at a HRT of 12 h and an OLR of 5.0 g COD/L/h. The average hydrogen content of biogas and COD reduction were 52% and 62%, respectively. The major soluble metabolites during hydrogen fermentation were butyric acid followed by acetic acid. It is concluded that the PEG-immobilized cell system developed in this work has great potential for continuous hydrogen production from real wastewater (POME) using the UASB reactor.
format	Article
author	Singh, Lakhveer Muhammad Faisal, Siddiqui Anwar, Ahmad Mohd Hasbi, Ab. Rahim Mimi Sakinah, A. M. Zularisam, A. W.
author_facet	Singh, Lakhveer Muhammad Faisal, Siddiqui Anwar, Ahmad Mohd Hasbi, Ab. Rahim Mimi Sakinah, A. M. Zularisam, A. W.
author_sort	Singh, Lakhveer
title	Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor
title_short	Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor
title_full	Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor
title_fullStr	Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor
title_full_unstemmed	Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor
title_sort	application of polyethylene glycol immobilized clostridium sp. ls2 for continuous hydrogen production from palm oil mill effluent in upflow anaerobic sludge blanket reactor
publisher	Elsevier Ltd
publishDate	2013
url	http://umpir.ump.edu.my/id/eprint/6243/ http://umpir.ump.edu.my/id/eprint/6243/ http://umpir.ump.edu.my/id/eprint/6243/ http://umpir.ump.edu.my/id/eprint/6243/1/fist-lakhveer-Application_of_polyethylene_abs_only.pdf
first_indexed	2023-09-18T22:01:50Z
last_indexed	2023-09-18T22:01:50Z
_version_	1777414449699749888

Application of Polyethylene Glycol Immobilized Clostridium sp. LS2 for Continuous Hydrogen Production From Palm Oil Mill Effluent in Upflow Anaerobic Sludge Blanket Reactor

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