Application of Adsorption Chromatography in the Papain Processing

A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow v...

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Main Authors:	He, Yuhai, Syed Mohd Saufi, Tuan Chik, Chong, Fui Chin
Format:	Article
Language:	English
Published:	Global Science and Technology Forum 2014
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf

id	ump-6260
recordtype	eprints
spelling	ump-62602018-03-26T07:33:42Z http://umpir.ump.edu.my/id/eprint/6260/ Application of Adsorption Chromatography in the Papain Processing He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin TP Chemical technology A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice. Global Science and Technology Forum 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf He, Yuhai and Syed Mohd Saufi, Tuan Chik and Chong, Fui Chin (2014) Application of Adsorption Chromatography in the Papain Processing. GSTF Journal of Chemical Sciences (JChem), 1 (2). pp. 9-15. ISSN 2339-5079 (Online) https://www.globalsciencejournals.com/article/10.7603%2Fs40837-014-0002-8 doi: 10.7603/s40837-014-0002-8
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin Application of Adsorption Chromatography in the Papain Processing
description	A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice.
format	Article
author	He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin
author_facet	He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin
author_sort	He, Yuhai
title	Application of Adsorption Chromatography in the Papain Processing
title_short	Application of Adsorption Chromatography in the Papain Processing
title_full	Application of Adsorption Chromatography in the Papain Processing
title_fullStr	Application of Adsorption Chromatography in the Papain Processing
title_full_unstemmed	Application of Adsorption Chromatography in the Papain Processing
title_sort	application of adsorption chromatography in the papain processing
publisher	Global Science and Technology Forum
publishDate	2014
url	http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf
first_indexed	2023-09-18T22:01:52Z
last_indexed	2023-09-18T22:01:52Z
_version_	1777414451631226880

Application of Adsorption Chromatography in the Papain Processing

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