Application of Adsorption Chromatography in the Papain Processing
A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow v...
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Global Science and Technology Forum
2014
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ump-62602018-03-26T07:33:42Z http://umpir.ump.edu.my/id/eprint/6260/ Application of Adsorption Chromatography in the Papain Processing He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin TP Chemical technology A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice. Global Science and Technology Forum 2014 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf He, Yuhai and Syed Mohd Saufi, Tuan Chik and Chong, Fui Chin (2014) Application of Adsorption Chromatography in the Papain Processing. GSTF Journal of Chemical Sciences (JChem), 1 (2). pp. 9-15. ISSN 2339-5079 (Online) https://www.globalsciencejournals.com/article/10.7603%2Fs40837-014-0002-8 doi: 10.7603/s40837-014-0002-8 |
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TP Chemical technology He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin Application of Adsorption Chromatography in the Papain Processing |
description |
A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa· s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice. |
format |
Article |
author |
He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin |
author_facet |
He, Yuhai Syed Mohd Saufi, Tuan Chik Chong, Fui Chin |
author_sort |
He, Yuhai |
title |
Application of Adsorption Chromatography in the Papain Processing |
title_short |
Application of Adsorption Chromatography in the Papain Processing |
title_full |
Application of Adsorption Chromatography in the Papain Processing |
title_fullStr |
Application of Adsorption Chromatography in the Papain Processing |
title_full_unstemmed |
Application of Adsorption Chromatography in the Papain Processing |
title_sort |
application of adsorption chromatography in the papain processing |
publisher |
Global Science and Technology Forum |
publishDate |
2014 |
url |
http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/ http://umpir.ump.edu.my/id/eprint/6260/1/Application_of_Adsorption_Chromatography_in_the_Papain_Processing.pdf |
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2023-09-18T22:01:52Z |
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2023-09-18T22:01:52Z |
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