Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa

Different accessions of basil introduced to Malaysia as seeds and cultivated at University Malaysia Pahang (UMP) farm and their essential oils extracted by steam distillation. Three chemotypes of these essential oils with estragole, linalool and methyl cinnamate as dominant compounds were evaluated...

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Main Authors:	Zuriah, Ismail, Azhari, H. Nour, Elhussein, Salah A. A.
Format:	Article
Language:	English English
Published:	Universiti Teknologi Malaysia 2012
Subjects:	Q Science (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/6266/ http://umpir.ump.edu.my/id/eprint/6266/ http://umpir.ump.edu.my/id/eprint/6266/1/Qualitative_and_quantitative_biological_studies_of_three_chemotypes_of_basil_essential_oils_grown_in_Malaysia_against_E.coli%2C_S.aureus_and_P._aeruginosa.pdf http://umpir.ump.edu.my/id/eprint/6266/3/fist-2012-zuriah-qualitative%20and%20quantitative.pdf

id	ump-6266
recordtype	eprints
spelling	ump-62662018-03-08T07:35:01Z http://umpir.ump.edu.my/id/eprint/6266/ Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa Zuriah, Ismail Azhari, H. Nour Elhussein, Salah A. A. Q Science (General) Different accessions of basil introduced to Malaysia as seeds and cultivated at University Malaysia Pahang (UMP) farm and their essential oils extracted by steam distillation. Three chemotypes of these essential oils with estragole, linalool and methyl cinnamate as dominant compounds were evaluated against three facultative anaerobic bacteria obtained from the National Pharmaceutical Control Bureau, Ministry of Health Malaysia: namely, Escherichia coli, Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) by qualitative and quantitative method. Different concentration ranged from 10 µL/mL to 1000 µL/mL of basil essential oil solutions prepared and tested against bacterial strain using agar well diffusion and quantitative methods. Minimum inhibitory concentration (MIC) was performed using broth microdilution plate. Eight different concentrations of serial two-fold dilutions ranged between 250 µL/mL and 1.95 µL/mL performed using Magellan software of Tecan Infinite Series M200 Pro microplate reader. In the result of agar well diffusion test, the zone of inhibition increased as the concentration of essential oil increased. The MIC was 7.81 µL/mL for all chemotype of the oils against E.coli and S.aureus while 15.63 µL/mL for P.aeruginosa of linalool-rich chemotype and 31.25 µL/mL for estragole and methyl cinnamate-rich chemotypes. The results obtained in this study were considered encouraging the potential of basil essential oil for medicinal uses as antibiotics and hygienic purposes as antibacterial products. Universiti Teknologi Malaysia 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6266/1/Qualitative_and_quantitative_biological_studies_of_three_chemotypes_of_basil_essential_oils_grown_in_Malaysia_against_E.coli%2C_S.aureus_and_P._aeruginosa.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/6266/3/fist-2012-zuriah-qualitative%20and%20quantitative.pdf Zuriah, Ismail and Azhari, H. Nour and Elhussein, Salah A. A. (2012) Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa. Malaysian Journal of Fundamental & Applied Sciences , 8 (2). pp. 101-106. ISSN 2289-5981 http://www.mjfas.utm.my/index.php/mjfas/article/view/131
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	Q Science (General)
spellingShingle	Q Science (General) Zuriah, Ismail Azhari, H. Nour Elhussein, Salah A. A. Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa
description	Different accessions of basil introduced to Malaysia as seeds and cultivated at University Malaysia Pahang (UMP) farm and their essential oils extracted by steam distillation. Three chemotypes of these essential oils with estragole, linalool and methyl cinnamate as dominant compounds were evaluated against three facultative anaerobic bacteria obtained from the National Pharmaceutical Control Bureau, Ministry of Health Malaysia: namely, Escherichia coli, Pseudomonas aeruginosa (Gram-negative) and Staphylococcus aureus (Gram-positive) by qualitative and quantitative method. Different concentration ranged from 10 µL/mL to 1000 µL/mL of basil essential oil solutions prepared and tested against bacterial strain using agar well diffusion and quantitative methods. Minimum inhibitory concentration (MIC) was performed using broth microdilution plate. Eight different concentrations of serial two-fold dilutions ranged between 250 µL/mL and 1.95 µL/mL performed using Magellan software of Tecan Infinite Series M200 Pro microplate reader. In the result of agar well diffusion test, the zone of inhibition increased as the concentration of essential oil increased. The MIC was 7.81 µL/mL for all chemotype of the oils against E.coli and S.aureus while 15.63 µL/mL for P.aeruginosa of linalool-rich chemotype and 31.25 µL/mL for estragole and methyl cinnamate-rich chemotypes. The results obtained in this study were considered encouraging the potential of basil essential oil for medicinal uses as antibiotics and hygienic purposes as antibacterial products.
format	Article
author	Zuriah, Ismail Azhari, H. Nour Elhussein, Salah A. A.
author_facet	Zuriah, Ismail Azhari, H. Nour Elhussein, Salah A. A.
author_sort	Zuriah, Ismail
title	Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa
title_short	Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa
title_full	Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa
title_fullStr	Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa
title_full_unstemmed	Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa
title_sort	qualitative and quantitative biological studies of three chemotypes of basil essential oils grown in malaysia against e.coli, s.aureus and p.aeruginosa
publisher	Universiti Teknologi Malaysia
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/6266/ http://umpir.ump.edu.my/id/eprint/6266/ http://umpir.ump.edu.my/id/eprint/6266/1/Qualitative_and_quantitative_biological_studies_of_three_chemotypes_of_basil_essential_oils_grown_in_Malaysia_against_E.coli%2C_S.aureus_and_P._aeruginosa.pdf http://umpir.ump.edu.my/id/eprint/6266/3/fist-2012-zuriah-qualitative%20and%20quantitative.pdf
first_indexed	2023-09-18T22:01:52Z
last_indexed	2023-09-18T22:01:52Z
_version_	1777414452368375808

Qualitative and Quantitative Biological Studies of Three Chemotypes of Basil Essential Oils Grown in Malaysia Against E.coli, S.aureus and P.aeruginosa

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