Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer

Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membr...

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Main Authors:	Muhammad Faisal, Siddiqui, Mimi Sakinah, A. M., Singh, Lakhveer, Zularisam, A. W.
Format:	Article
Language:	English English
Published:	Elsevier B.V. 2012
Subjects:	TA Engineering (General). Civil engineering (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/1/Targeting_N-acyl-homoserine_lactones_to_mitigate_membrane_biofouling_based_on_quorum_sensing_using_a_biofouling_reducer.pdf http://umpir.ump.edu.my/id/eprint/6817/4/Targeting%20N.pdf

id	ump-6817
recordtype	eprints
spelling	ump-68172018-01-17T01:05:07Z http://umpir.ump.edu.my/id/eprint/6817/ Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer Muhammad Faisal, Siddiqui Mimi Sakinah, A. M. Singh, Lakhveer Zularisam, A. W. TA Engineering (General). Civil engineering (General) Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membrane biofouling. This study investigated the role of Piper betle extract (PBE) as an anti-QS agent to mitigate membrane biofouling. Results demonstrated the occurrence of the N-acyl-homoserine-lactone (AHL) autoinducers (AIs), correlate QS activity and membrane biofouling mitigation. The AIs production in bioreactor was confirmed using an indicator strain Agrobacterium tumefaciens (NTL4) harboring plasmid pZLR4. Moreover, three different AHLs were found in biocake using thin layer chromatographic analysis. An increase in extracellular polymeric substances (EPS) and transmembrane pressure (TMP) was observed with AHL activity of the biocake during continuous MBR operation, which shows that membrane biofouling was in close relationship with QS activity. PBE was verified to mitigate membrane biofouling via inhibiting AIs production. SEM analysis further confirmed the effect of PBE on EPS and biofilm formation. These results exhibited that PBE could be a novel agent to target AIs for mitigation of membrane biofouling. Further work can be carried out to purify the active compound of Piper betle extract to target the QS to mitigate membrane biofouling. Elsevier B.V. 2012 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/6817/1/Targeting_N-acyl-homoserine_lactones_to_mitigate_membrane_biofouling_based_on_quorum_sensing_using_a_biofouling_reducer.pdf application/pdf en http://umpir.ump.edu.my/id/eprint/6817/4/Targeting%20N.pdf Muhammad Faisal, Siddiqui and Mimi Sakinah, A. M. and Singh, Lakhveer and Zularisam, A. W. (2012) Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer. Journal of Biotechnology, 161. pp. 190-197. ISSN 0168-1656 http://dx.doi.org/10.1016/j.jbiotec.2012.06.029 doi: 10.1016/j.jbiotec.2012.06.029
repository_type	Digital Repository
institution_category	Local University
institution	Universiti Malaysia Pahang
building	UMP Institutional Repository
collection	Online Access
language	English English
topic	TA Engineering (General). Civil engineering (General)
spellingShingle	TA Engineering (General). Civil engineering (General) Muhammad Faisal, Siddiqui Mimi Sakinah, A. M. Singh, Lakhveer Zularisam, A. W. Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer
description	Exploring novel biological anti-quorum sensing (QS) agents to control membrane biofouling is of great worth in order to allow sustainable performance of membrane bioreactors (MBRs) for wastewater treatment. In recent studies, QS inhibitors have provided evidence of alternative route to control membrane biofouling. This study investigated the role of Piper betle extract (PBE) as an anti-QS agent to mitigate membrane biofouling. Results demonstrated the occurrence of the N-acyl-homoserine-lactone (AHL) autoinducers (AIs), correlate QS activity and membrane biofouling mitigation. The AIs production in bioreactor was confirmed using an indicator strain Agrobacterium tumefaciens (NTL4) harboring plasmid pZLR4. Moreover, three different AHLs were found in biocake using thin layer chromatographic analysis. An increase in extracellular polymeric substances (EPS) and transmembrane pressure (TMP) was observed with AHL activity of the biocake during continuous MBR operation, which shows that membrane biofouling was in close relationship with QS activity. PBE was verified to mitigate membrane biofouling via inhibiting AIs production. SEM analysis further confirmed the effect of PBE on EPS and biofilm formation. These results exhibited that PBE could be a novel agent to target AIs for mitigation of membrane biofouling. Further work can be carried out to purify the active compound of Piper betle extract to target the QS to mitigate membrane biofouling.
format	Article
author	Muhammad Faisal, Siddiqui Mimi Sakinah, A. M. Singh, Lakhveer Zularisam, A. W.
author_facet	Muhammad Faisal, Siddiqui Mimi Sakinah, A. M. Singh, Lakhveer Zularisam, A. W.
author_sort	Muhammad Faisal, Siddiqui
title	Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer
title_short	Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer
title_full	Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer
title_fullStr	Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer
title_full_unstemmed	Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer
title_sort	targeting n-acyl-homoserine-lactones to mitigate membrane biofouling based on quorum sensing using a biofouling reducer
publisher	Elsevier B.V.
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/ http://umpir.ump.edu.my/id/eprint/6817/1/Targeting_N-acyl-homoserine_lactones_to_mitigate_membrane_biofouling_based_on_quorum_sensing_using_a_biofouling_reducer.pdf http://umpir.ump.edu.my/id/eprint/6817/4/Targeting%20N.pdf
first_indexed	2023-09-18T22:02:56Z
last_indexed	2023-09-18T22:02:56Z
_version_	1777414518690807808

Targeting N-acyl-homoserine-lactones to Mitigate Membrane Biofouling based on Quorum Sensing using a Biofouling Reducer

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